nach oben

Lasers in Medical Science

Erschienen in:

01.03.2009 | Original article

Low-level laser therapy modulates cyclo-oxygenase-2 expression during bone repair in rats

verfasst von: Mariza A. Matsumoto, Rafael V. Ferino, Giovanni F. Monteleone, Daniel A. Ribeiro

Erschienen in: Lasers in Medical Science | Ausgabe 2/2009

Einloggen, um Zugang zu erhalten

Abstract

The goal of this study was to analyze the role of cyclo-oxygenase-2 following bone repair in rats submitted to low-level laser therapy. A total of 48 rats underwent surgery to inflict bone defects in their tibias having been randomly distributed into two groups: negative control and laser exposed group, i.e., the animals were treated with low-level laser therapy by means of gallium arsenide laser at 16 J/cm². The animals were killed after 48 h, 7 days, 14 days, or 21 days. The tibias were removed for morphological, morphometric, and immunohistochemistry analysis for cyclo-oxygenase-2. Statistical significant differences (P < 0.05) were observed in the quality of bone repair and quantity of formed bone between groups 14 days after surgery in the laser exposed group. In the same way, cyclo-oxygenase-2 immunoreactivity was more intense in bone cells for intermediate periods evaluated in this group. Taken together, such results suggest that low-level laser therapy is able to improve bone repair in the tibia of rats after 14 days of surgery as a result of an up-regulation for cyclo-oxygenase-2 expression in bone cells.

Hammond A (2004) Rehabilitation in rheumatoid arthritis: a critical review. Musculoskelet Care 2:135–151CrossRef

Cetiner S, Kahraman SA, Yucetas S (2006) Evaluation of low-level laser therapy in the treatment of temporomandibular disorders. Photomed Laser Surg 24:637–641PubMedCrossRef

Faria Amorim JC, Sousa GR, Silveira Lde B, Prates RA, Pinotti M, Ribeiro MS (2006) Clinical study of the gingiva healing after gingivectomy and low-level laser therapy. Photomed Laser Surg 24:588–594CrossRef

Landthaler M, Hohenleutner U (2006) Laser therapy of vascular lesions. Photodermatol Photoimmunol Photomed 22:324–332PubMedCrossRef

Turner J, Jode L (1999) Low level laser therapy. Prima Books, Stockholm

Kipshidge N, Nikolaychik V, Keelan MH (2001) Low-power helium:neon laser irradiation enhances production of vascular endothelial growth factor and promotes growth of endothelial cells in vitro. Lasers Surg Med 28:355–364CrossRef

Chen WR, Liu H, Ritchey JW, Bartels KE, Lucroy MD, Nordquist RE (2002) Effect of different components of laser immunotherapy in treatment of metastatic tumors in rats. Cancer Res 62:4295–4299PubMed

Dube A, Bansal H, Gupta PK (2003) Modulation of macrophage structure and function by low level He-Ne laser irradiation. Photochem Photobiol Sci 2:851–855PubMedCrossRef

Lan CC, Wu CS, Chiou MH, Hsieh PC, Yu HS (2006) Low-energy helium-neon laser induces locomotion of the immature melanoblasts and promotes melanogenesis of the more differentiated melanoblasts: recapitulation of vitiligo repigmentation in vitro. J Invest Dermatol 126:2119–2126PubMedCrossRef

10.

Bayat M, Vasheghani MM, Razavi N, Taheri S, Rakhshan M (2005) Effect of low-level laser therapy on the healing of second-degree burns in rats: a histological and microbiological study. J Photochem Photobiol B 78:171–177PubMedCrossRef

11.

Nissan J, Assif D, Gross MD, Yaffe A, Binderman I (2006) Effect of low intensity laser irradiation on surgically created bony defects in rats. J Oral Rehabil 33:619–924PubMedCrossRef

12.

da Silva RV, Camilli JA (2006) Repair of bone defects treated with autogenous bone graft and low-power laser. J Craniofac Surg 17:297–301PubMedCrossRef

13.

Heckman JD, Sarasohn-Kahn J (1997) The economics of treating tibia fractures. The cost of delayed unions. Bull Hosp Joint Dis 56:63–72

14.

Nicola RA, Jorgetti V, Rigau J, Pacheco MT, dos Reis LM, Zângaro RA (2003) Effect of low-power GaAlAs laser (660 nm) on bone structure and cell activity: an experimental animal study. Lasers Med Sci 18:89–94PubMedCrossRef

15.

Lirani-Galvao AP, Jorgetti V, da Silva OL (2006) Comparative study of how low-level laser therapy and low-intensity pulsed ultrasound affect bone repair in rats. Photomed Laser Surg 24:735–740PubMedCrossRef

16.

Kargman S, Charleson S, Cartwright M, Frank J, Riendeau D, Mancini J, Evans J, O’Neill G (1996) Characterization of prostaglandin G/H synthase 1 and 2 in rat, dog, monkey, and human gastrointestinal tracts. Gastroenterology 111:445–454PubMedCrossRef

17.

Dempke W, Rie C, Grothey A, Schmoll HJ (2001) Cyclooxygenase-2: a novel target for cancer chemotherapy? J Cancer Res Clin Oncol 127:411–417PubMedCrossRef

18.

Miranda SR, Filho HN, Marques Padovan LE, Ribeiro DA, Nicolielo D, Matsumoto MA (2006) Use of platelet-rich plasma under autogenous onlay bone grafts. Clin Oral Implant Res 17:694–699CrossRef

19.

Gerbi ME, Pinheiro AL, Marzola C, Limeira Júnior Fde A, Ramalho LM, Ponzi EA, Soares AO, Carvalho LC, Lima HV, Gonçalves TO (2005) Assessment of bone repair associated with the use of organic bovine bone and membrane irradiated at 830 nm. Photomed Laser Surg 23:382–388PubMedCrossRef

20.

Khadra M, Kasem N, Haanaes HR, Ellingsen JE, Lyngstadaas SP (2004) Enhancement of bone formation in rat calvarial bone defects using low-level laser therapy. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 97:693–700PubMedCrossRef

21.

Coombe AR, Ho CT, Darendeliler MA, Hunter N, Philips JR, Chapple CC, Yum LW (2001) The effects of low level laser irradiation on osteoblastic cells. Clin Orthod Res 4:3–14PubMedCrossRef

22.

Khadra M (2005) The effect of low level laser irradiation on implant-tissue interaction. In vivo and in vitro studies. Swed Dent J Suppl 172:1–63PubMed

23.

Ozawa Y, Shimizu N, Kariya G, Abiko Y (1998) Low-energy laser irradiation stimulates bone nodule formation at early stages of cell culture in rat calvarial cells. Bone 22:347–354PubMedCrossRef

24.

Shibata M, Kodani I, Osaki M, Araki K, Adachi H, Ryoke K, Ito H (2005) Cyclo-oxygenase-1 and -2 expression in human oral mucosa, dysplasias and squamous cell carcinomas and their pathological significance. Oral Oncol 41:304–312CrossRef

25.

Tsujii M, Kawano S, Tsuji S, Sawaoka H, Hori M, DuBois RN (1998) Cyclooxygenase regulates angiogenesis induced by colon cancer cells. Cell 93:705–716PubMedCrossRef

26.

Wang W, Bergh A, Damber JE (2007) Increased expression of CCAAT/enhancer-binding protein beta in proliferative inflammatory atrophy of the prostate: relation with the expression of COX-2, the androgen receptor, and presence of focal chronic inflammation. Prostate 67:1238–1246PubMedCrossRef

27.

Sato Y, Arai N, Negishi A, Ohya K (1997) Expression of cyclooxygenase genes and involvement of endogenous prostaglandin during osteogenesis in the rat tibial bone marrow cavity. J Med Dent Sci 44:81–92PubMed

28.

Zhang X, Schwarz EM, Young DA, Puzas E, Rosier RN, O’Keefe RJ (2002) Cyclooxygenase-2 regulates mesenchymal cell differentiation into the osteoblast lineage and is critically involved in bone repair. J Clin Invest 109:1405–1415PubMed

29.

Li J, Burr DB, Turner CH (2002) Suppression of prostaglandin synthesis with NS-398 has different effects on endocortical and periosteal bone formation induced by mechanical loading. Calcif Tissue Int 70:320–329PubMedCrossRef

30.

Forwood MR (1996) Inducible cyclo-oxygenase (COX-2) mediates the induction of bone formation by mechanical loading in vivo. J Bone Miner Res 11:1688–1693PubMedCrossRef

Titel: Low-level laser therapy modulates cyclo-oxygenase-2 expression during bone repair in rats
verfasst von: Mariza A. Matsumoto
Rafael V. Ferino
Giovanni F. Monteleone
Daniel A. Ribeiro
Publikationsdatum: 01.03.2009
Verlag: Springer-Verlag
Erschienen in: Lasers in Medical Science / Ausgabe 2/2009
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-008-0544-4

Springer Medizin

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 2/2009

Symptoms treatment for allergic rhinitis using diode laser: results after 6-year follow-up

Laser phototherapy effect on protein metabolism parameters of rat salivary glands

Review of methodological developments in laser Doppler flowmetry

Can carbon dioxide laser substitute moxibustion?

Adverse effects reported in pulsed dye laser treatment for port wine stains

Results of fractional ablative facial skin resurfacing with the erbium:yttrium-aluminium-garnet laser 1 week and 2 months after one single treatment in 30 patients