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Lasers in Medical Science

Erschienen in:

01.07.2009 | Original Article

Effect of diode laser on enzymatic activity of parotid glands of diabetic rats

verfasst von: Alyne Simões, Emily Ganzerla, Paula Mochidome Yamaguti, Carlos de Paula Eduardo, José Nicolau

Erschienen in: Lasers in Medical Science | Ausgabe 4/2009

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Abstract

The aim of this study was to evaluate the effect of laser irradiation (LI) on enzymatic activities of amylase, catalase and peroxidase in the parotid glands (PG) of diabetic and non-diabetic rats. Ninety-six female rats were divided into eight groups: D0; D5; D10; D20 and C0; C5; C10; C20, respectively. Diabetes was induced by administration of streptozotocin and confirmed later by the glycemia results. Twenty-nine (29) days after the induction, the PGs of groups D5 and C5; D10 and C10; D20 and C20, were irradiated with 5 J/cm², 10 J/cm² and 20 J/cm² of laser diode (660 nm/100 mW) respectively. On the following day, the rats were euthanized and the enzymatic activity in the PGs was measured. Diabetic rats that had not been irradiated (group D0) showed higher catalase activity (P < 0.05) than those in group C0 (0.14 ± 0.02 U/mg protein and 0.10 ± 0.03 U/mg protein, respectively). However, laser irradiation of 5 J/cm² and 20 J/cm² decreased the catalase activity of the diabetic groups (D5 and D20) to non-diabetic values (P > 0.05). Based on the results of this study, LI decreased catalase activity in the PGs of diabetic rats.

No authors listed (2002) Report of the Expert Committee on the Diagnosis and Classification of Diabetes Mellitus. Diabetes Care 25:S5–S20. doi:10.2337/diacare.25.2007.S5

Bridges RB (1981) Salivary glands and saliva. In: Roth GI, Calmes R (eds) Oral biology, Mosby, St Louis

Pedersen AM, Bardow A, Jensen SB, Nauntofte B (2002) Saliva and gastrointestinal functions of taste, mastication, swallowing and digestion. Oral Dis 8:117–129. doi:10.1034/j.1601–0825.2002.02851.x PubMedCrossRef

von Bultzingslowen I, Sollecito TP, Fox PC, Daniels T, Jonsson R, Lockhart PB, Wray D, Brennan MT, Carrozzo M, Gandera B, Fujibayashi T, Navazesh M, Rhodus NL, Schiodt M (2007) Salivary dysfunction associated with systemic diseases: systematic review and clinical management recommendations. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 103 [Suppl]:S57 e1–15

Moore PA, Guggenheimer J, Etzel KR, Weyant RJ, Orchard T (2001) Type 1 diabetes mellitus, xerostomia, and salivary flow rates. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 92:281–291. doi:10.1067/moe.2001.117815 PubMedCrossRef

Anderson LC (1991) Effects of diabetes and dietary manipulation on rat parotid gland secretory response to sympathetic nerve stimulation. Comp Biochem Physiol A 98:363–366. doi:10.1016/0300–9629(91)90548-Q PubMedCrossRef

Nogueira FN, Carvalho AM, Yamaguti PM, Nicolau J (2005) Antioxidant parameters and lipid peroxidation in salivary glands of streptozotocin-induced diabetic rats. Clin Chim Acta 353:133–139. doi:10.1016/j.cccn.2004.11.004 PubMedCrossRef

Manfredi M, McCullough MJ, Vescovi P, Al-Kaarawi ZM, Porter SR (2004) Update on diabetes mellitus and related oral diseases. Oral Dis 10:187–200. doi:10.1111/j.1601–0825.2004.01019.x PubMedCrossRef

Nicolau J, Matos JA, Souza DN, Neves LB, Lopes AC (2005) Altered glycogen metabolism in the submandibular and parotid salivary glands of rats with streptozotocin-induced diabetes. J Oral Sci 47:111–116. doi:10.2334/josnusd.47.111 PubMedCrossRef

10.

Gavish L, Asher Y, Becker Y, Kleinman Y (2004) Low level laser irradiation stimulates mitochondrial membrane potential and disperses subnuclear promyelocytic leukemia protein. Lasers Surg Med 35:369–376. doi:10.1002/lsm.20108 PubMedCrossRef

11.

Cowen D, Tardieu C, Schubert M, Peterson D, Resbeut M, Faucher C et al (1997) Low energy helium-neon laser in the prevention of oral mucositis in patients undergoing bone marrow transplant: results of a double blind randomized trial. Int J Radiat Oncol Biol Phys 38:697–703. doi:10.1016/S0360–3016(97)00076-X PubMedCrossRef

12.

Simoes A, Nicolau J, de Souza DN, Ferreira LS, de Paula Eduardo C, Apel C et al (2008) Effect of defocused infrared diode laser on salivary flow rate and some salivary parameters of rats. Clin Oral Investig 12:25–30. doi:10.1007/s00784–007–0135-y PubMedCrossRef

13.

Simoes A, Siqueira WL, Lamers ML, Santos MF, Eduardo CP, Nicolau J (2008) Laser phototherapy effect on protein metabolism parameters of rat salivary glands. Lasers Med Sci. doi:10.1007/s10103–008–0548–0

14.

Fisher EH, Stein E (1961) a-Amylase from human saliva. Biochem Prep 8:27–33

15.

Aebi H (1984) Catalase in vitro. In: Methods Enzymol. Verlag Chemie, Winheim/Bergstr., pp.121–126

16.

Pinkstaff CA (1998) Salivary gland sexual dimorphism: a brief review. Eur J Morphol 36 [Suppl]:31–34PubMed

17.

Greenspan FS, Gardner DG (2004) Basic and clinical endocrinology, McGraw-Hill, New York

18.

Szczepanski A, Mednieks MI, Hand AR (1998) Expression and distribution of parotid secretory proteins in experimental diabetes. Eur J Morphol 36 [Suppl]:240–246PubMed

19.

Oberley LW (1988) Free radicals and diabetes. Free Radic Biol Med 5:113–124. doi:10.1016/0891–5849(88)90036–6 PubMedCrossRef

20.

Kakkar R, Kalra J, Mantha SV, Prasad K (1995) Lipid peroxidation and activity of antioxidant enzymes in diabetic rats. Mol Cell Biochem 151:113–119. doi:10.1007/BF01322333 PubMedCrossRef

21.

Anderson LC (1983) Effects of alloxan diabetes and insulin in vivo on rat parotid gland. Am J Physiol 245:G431–G437PubMed

22.

Gumieniczek A, Hopkala H, Wojtowicz Z, Nieradko M (2001) Differences in antioxidant status in skeletal muscle tissue in experimental diabetes. Clin Chim Acta 314:39–45. doi:10.1016/S0009–8981(01)00680–5 PubMedCrossRef

23.

Fridovich I (1995) Superoxide radical and superoxide dismutases. Annu Rev Biochem 64:97–112. doi:10.1146/annurev.bi.64.070195.000525 PubMedCrossRef

24.

Karageuzyan KG, Sekoyan ES, Karagyan AT, Pogosyan NR, Manucharyan GG, Sekoyan AE et al (1998) Phospholipid pool, lipid peroxidation, and superoxide dismutase activity under various types of oxidative stress of the brain and the effect of low-energy infrared laser irradiation. Biochemistry (Mosc) 63:1226–1232

25.

Kirkman HN, Gaetani GF (2007) Mammalian catalase: a venerable enzyme with new mysteries. Trends Biochem Sci 32:44–50. doi:10.1016/j.tibs.2006.11.003 PubMedCrossRef

26.

Ihalin R, Loimaranta V, Tenovuo J (2006) Origin, structure, and biological activities of peroxidases in human saliva. Arch Biochem Biophys 445:261–268. doi:10.1016/j.abb.2005.07.004 PubMedCrossRef

27.

Dobrosielski-Vergona K (1993) Biology of salivary glands, CRC Press, Boca Raton, FL

28.

Makinen K (1989) Salivary enzymes in human saliva: clinical chemistry and microbiology, CRC Press, Boca Raton, Fla

Titel: Effect of diode laser on enzymatic activity of parotid glands of diabetic rats
verfasst von: Alyne Simões
Emily Ganzerla
Paula Mochidome Yamaguti
Carlos de Paula Eduardo
José Nicolau
Publikationsdatum: 01.07.2009
Verlag: Springer-Verlag
Erschienen in: Lasers in Medical Science / Ausgabe 4/2009
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-008-0619-2

Springer Medizin

Abstract

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