Skip to main content
Erschienen in: Lasers in Medical Science 6/2009

01.11.2009 | Original Article

Influence of laser (660 nm) on functional recovery of the sciatic nerve in rats following crushing lesion

verfasst von: Ana Carulina Guimarães Belchior, Filipe Abdalla dos Reis, Renata Amadei Nicolau, Iandara Schettert Silva, Daniel M. Perreira, Paulo de Tarso Camillo de Carvalho

Erschienen in: Lasers in Medical Science | Ausgabe 6/2009

Einloggen, um Zugang zu erhalten

Abstract

With the aim of accelerating the regenerative processes, the objective was to study the influence of gallium–aluminum–arsenide (GaAlAs) laser (660 nm) on functional and histomorphological recovery of the sciatic nerve in rats. The sciatic nerves of 12 Wistar rats were crushed divided into two groups: control and laser therapy. For the latter, GaAlAs laser was utilized (660 nm, 4 J/cm2, 26.3 mW and 0.63 cm2 beam), at three equidistant points on the lesion, for 20 days. Comparison of the sciatic functional index (SFI) showed that there was a significant difference only between the pre-lesion value of the laser therapy group and that after the 21st day in the control group. It was concluded that the parameters and methods utilized demonstrated positive results regarding the SFI over the time period evaluated.
Literatur
1.
Zurück zum Zitat Rochkind S, Quaknine GE (1992) New trend in neuroscience: low-power laser effect on peripheral and central nervous system (basic science, preclinical and clinical studies). Neurol Res 14:2–11PubMed Rochkind S, Quaknine GE (1992) New trend in neuroscience: low-power laser effect on peripheral and central nervous system (basic science, preclinical and clinical studies). Neurol Res 14:2–11PubMed
7.
Zurück zum Zitat Rochkind S, Nissan M, Alon M, Shamir M, Salame K (2001) Effects of laser irradiation on the spinal cord for the regeneration of crushed peripheral nerve in rats. Lasers Surg Med 28:216–219. doi:10.1002/lsm.1041 CrossRefPubMed Rochkind S, Nissan M, Alon M, Shamir M, Salame K (2001) Effects of laser irradiation on the spinal cord for the regeneration of crushed peripheral nerve in rats. Lasers Surg Med 28:216–219. doi:10.​1002/​lsm.​1041 CrossRefPubMed
8.
Zurück zum Zitat Oliveira LS, Sobral LL, Takeda SY, Betini J, Guirro RR, Somazz MC, Teodori RM (2008) Electrical stimulation and swimming in the acute phase of axonotmesis: their influence on nerve regeneration and functional recovery. Rev Neurol 47:11–15PubMed Oliveira LS, Sobral LL, Takeda SY, Betini J, Guirro RR, Somazz MC, Teodori RM (2008) Electrical stimulation and swimming in the acute phase of axonotmesis: their influence on nerve regeneration and functional recovery. Rev Neurol 47:11–15PubMed
11.
Zurück zum Zitat Kozhakhmetov AN (1993) The evaluation of the adequacy of anesthesia during delivery (in Russian). Akush Ginekol (Mosk) 2:36–39 Kozhakhmetov AN (1993) The evaluation of the adequacy of anesthesia during delivery (in Russian). Akush Ginekol (Mosk) 2:36–39
12.
Zurück zum Zitat Almeida EMP, Nunes N, Fantinatti AP, Santos PSP, Bolzan AA, Rezende ML (2000) Efeitos cardiorrespiratórios da associação de tiletamina/zolazepam em cães (Canis familiaris) pré-tratados ou não pela acepromazina. Braz J Vet Anim Sci 37:45–51 Almeida EMP, Nunes N, Fantinatti AP, Santos PSP, Bolzan AA, Rezende ML (2000) Efeitos cardiorrespiratórios da associação de tiletamina/zolazepam em cães (Canis familiaris) pré-tratados ou não pela acepromazina. Braz J Vet Anim Sci 37:45–51
14.
15.
Zurück zum Zitat Beau JML, Ellisman MH, Powell HC (1988) Ultrastructural and morphometric analysis of long-term peripheral nerve regeneration through silicone tubes. J Neurocytol 17:161–172. doi:10.1007/BF01674203 CrossRefPubMed Beau JML, Ellisman MH, Powell HC (1988) Ultrastructural and morphometric analysis of long-term peripheral nerve regeneration through silicone tubes. J Neurocytol 17:161–172. doi:10.​1007/​BF01674203 CrossRefPubMed
18.
Zurück zum Zitat Lent R (2004) Cem bilhões de neurônios: conceitos fundamentais da neurociência, 3rd edn. Atheneu, São Paulo, p 135 Lent R (2004) Cem bilhões de neurônios: conceitos fundamentais da neurociência, 3rd edn. Atheneu, São Paulo, p 135
19.
Zurück zum Zitat Rochkind S, Nissan M, Lubart R, Avram J, Bartal A (1988) The in-vivo nerve response to direct low-energy-laser irradiation. Acta Neurochir (Wien) 94:74–77. doi:10.1007/BF01406620 CrossRef Rochkind S, Nissan M, Lubart R, Avram J, Bartal A (1988) The in-vivo nerve response to direct low-energy-laser irradiation. Acta Neurochir (Wien) 94:74–77. doi:10.​1007/​BF01406620 CrossRef
23.
Zurück zum Zitat Bagis S, Comelekoglu U, Sahin G, Buyukakilli B, Erdogan C, Kanik A (2002) Acute electrophysiologic effect of pulsed gallium-arsenide low energy laser irradiation on configuration of compound nerve action potential and nerve excitability. Lasers Surg Med 30:376–380. doi:10.1002/lsm.10057 CrossRefPubMed Bagis S, Comelekoglu U, Sahin G, Buyukakilli B, Erdogan C, Kanik A (2002) Acute electrophysiologic effect of pulsed gallium-arsenide low energy laser irradiation on configuration of compound nerve action potential and nerve excitability. Lasers Surg Med 30:376–380. doi:10.​1002/​lsm.​10057 CrossRefPubMed
25.
Zurück zum Zitat Rochkind S, Nissan M, Razon N, Schwartz M, Bartal A (1986) Electrophysiological effect of HeNe laser on normal and injured sciatic nerve in the rat. Acta Neurochir (Wien) 83:125–130. doi:10.1007/BF01402391 CrossRef Rochkind S, Nissan M, Razon N, Schwartz M, Bartal A (1986) Electrophysiological effect of HeNe laser on normal and injured sciatic nerve in the rat. Acta Neurochir (Wien) 83:125–130. doi:10.​1007/​BF01402391 CrossRef
26.
Zurück zum Zitat Sotelo PR, Sosa VMR, Martínez RT, Barry HG (1996) El laser helio-neon en la regeneración del nervio ciático seccionado y suturado. Rev Cubana Cir 35:00 Sotelo PR, Sosa VMR, Martínez RT, Barry HG (1996) El laser helio-neon en la regeneración del nervio ciático seccionado y suturado. Rev Cubana Cir 35:00
27.
Zurück zum Zitat Schwartz F, Brodie C, Appel E, Kazimirsky G, Shainberg A (2002) Effect of helium/neon laser irradiation on nerve growth factor synthesis and secretion in skeletal muscle cultures. J Photochem Photobiol B 66:195–200. doi:10.1016/S1011-1344(02)00267-1 CrossRefPubMed Schwartz F, Brodie C, Appel E, Kazimirsky G, Shainberg A (2002) Effect of helium/neon laser irradiation on nerve growth factor synthesis and secretion in skeletal muscle cultures. J Photochem Photobiol B 66:195–200. doi:10.​1016/​S1011-1344(02)00267-1 CrossRefPubMed
28.
Zurück zum Zitat Snyder SK, Byrnes KR, Borke RC, Sanches A, Anders JJ (2002) Quantitation of calcitonin gene-related peptide mRNA and neuronal cell death in facial motor nuclei following axotomy and 633 nm low power laser. Lasers Surg Med 31:216–222. doi:10.1002/lsm.10098 CrossRefPubMed Snyder SK, Byrnes KR, Borke RC, Sanches A, Anders JJ (2002) Quantitation of calcitonin gene-related peptide mRNA and neuronal cell death in facial motor nuclei following axotomy and 633 nm low power laser. Lasers Surg Med 31:216–222. doi:10.​1002/​lsm.​10098 CrossRefPubMed
29.
Zurück zum Zitat Khullar SM, Brodin P, Fristad I, Kvinnsland IH (1999) Enhanced sensory reinnervation of dental target tissues in rats following low level laser (LLL) irradiation. Lasers Med Sci 14:177–184. doi:10.1007/s101030050082 CrossRef Khullar SM, Brodin P, Fristad I, Kvinnsland IH (1999) Enhanced sensory reinnervation of dental target tissues in rats following low level laser (LLL) irradiation. Lasers Med Sci 14:177–184. doi:10.​1007/​s101030050082 CrossRef
31.
Zurück zum Zitat Shamir MH, Rochkind S, Sandbank J, Alon M (2001) Double-blind randomized study evaluating regeneration of the rat transected sciatic nerve after suturing and postoperative low-power laser treatment. J Reconstr Microsurg 17:133–137. doi:10.1055/s-2001-12702 CrossRefPubMed Shamir MH, Rochkind S, Sandbank J, Alon M (2001) Double-blind randomized study evaluating regeneration of the rat transected sciatic nerve after suturing and postoperative low-power laser treatment. J Reconstr Microsurg 17:133–137. doi:10.​1055/​s-2001-12702 CrossRefPubMed
32.
33.
Zurück zum Zitat Gigo-Benato D, Geuna S, Rodrigues AC, Fornaro PTM, Boux E, Battiston B, Giacobini-Robecchi MG (2004) Low-power laser biostimulation enhances nerve repair after end-to-side neurorrhaphy: a double-blind randomized study in the rat median nerve model. Lasers Med Sci 19:57–65. doi:10.1007/s10103-004-0300-3 CrossRefPubMed Gigo-Benato D, Geuna S, Rodrigues AC, Fornaro PTM, Boux E, Battiston B, Giacobini-Robecchi MG (2004) Low-power laser biostimulation enhances nerve repair after end-to-side neurorrhaphy: a double-blind randomized study in the rat median nerve model. Lasers Med Sci 19:57–65. doi:10.​1007/​s10103-004-0300-3 CrossRefPubMed
34.
Zurück zum Zitat Nicolau RA, Martinez MS, Rigau J, Tomàs J (2004) Effect of power 655 nm diode laser irradiation on the neuromuscular junctions of the mouse diaphragm. Lasers Surg Med 34:277−284. doi:10.1002/lsm.20006 CrossRefPubMed Nicolau RA, Martinez MS, Rigau J, Tomàs J (2004) Effect of power 655 nm diode laser irradiation on the neuromuscular junctions of the mouse diaphragm. Lasers Surg Med 34:277−284. doi:10.​1002/​lsm.​20006 CrossRefPubMed
35.
Zurück zum Zitat Byrnes KR, Waynant RW, Ilev IK, Wu X, Barna L, Smith K, Heckert R, Gerst H, Anders JJ (2005) Light promotes regeneration and functional recovery and alters the immune response after spinal cord injury. Lasers Surg Med 36:171−185. doi:10.1002/lsm.20143 CrossRefPubMed Byrnes KR, Waynant RW, Ilev IK, Wu X, Barna L, Smith K, Heckert R, Gerst H, Anders JJ (2005) Light promotes regeneration and functional recovery and alters the immune response after spinal cord injury. Lasers Surg Med 36:171−185. doi:10.​1002/​lsm.​20143 CrossRefPubMed
36.
Zurück zum Zitat Bagis S, Comelekoglu U, Coskun B, Milcan A, Buyukakilli B, Sahin G, Ozisik S, Erdogan C (2003) No effect of GA-AS (904 nm) laser irradiation on the intact skin of the injured rat sciatic nerve. Lasers Med Sci 18:83–88. doi:10.1007/s10103-003-0258-6 CrossRefPubMed Bagis S, Comelekoglu U, Coskun B, Milcan A, Buyukakilli B, Sahin G, Ozisik S, Erdogan C (2003) No effect of GA-AS (904 nm) laser irradiation on the intact skin of the injured rat sciatic nerve. Lasers Med Sci 18:83–88. doi:10.​1007/​s10103-003-0258-6 CrossRefPubMed
37.
Zurück zum Zitat Rochkind S, Barr-Nea L, Bartal A, Nissan M, Lubart R, Razon N (1988) New methods of treatment of severely injured sciatic nerve and spinal cord: an experimental study. Acta Neurochir (Wien) 43:91–93 b Rochkind S, Barr-Nea L, Bartal A, Nissan M, Lubart R, Razon N (1988) New methods of treatment of severely injured sciatic nerve and spinal cord: an experimental study. Acta Neurochir (Wien) 43:91–93 b
38.
Zurück zum Zitat Chelyshev IA, Kubitskii AA, Plakseichuk A (1996) Regeneratsiia nervnykh volokon pri obluchenii nizkointensivnymi lazerami. Morfologiia 110:47–50PubMed Chelyshev IA, Kubitskii AA, Plakseichuk A (1996) Regeneratsiia nervnykh volokon pri obluchenii nizkointensivnymi lazerami. Morfologiia 110:47–50PubMed
41.
Zurück zum Zitat Varejão ASP, Cabrita AM, Geuna S, Melo-Pinto P, Filipe VM, Gramsbergen A, Meek MF (2003) Toe out angle: a functional index for the evaluation of the sciatic nerve recovery in the rat model. Exp Neurol 183:695–699. doi:10.1016/S0014-4886(03)00208-5 CrossRefPubMed Varejão ASP, Cabrita AM, Geuna S, Melo-Pinto P, Filipe VM, Gramsbergen A, Meek MF (2003) Toe out angle: a functional index for the evaluation of the sciatic nerve recovery in the rat model. Exp Neurol 183:695–699. doi:10.​1016/​S0014-4886(03)00208-5 CrossRefPubMed
42.
44.
Zurück zum Zitat Karu TI (1988) Molecular mechanisms of the therapeutic effect of low-intensity laser irradiation. Lasers Life Sci 2:53–74 Karu TI (1988) Molecular mechanisms of the therapeutic effect of low-intensity laser irradiation. Lasers Life Sci 2:53–74
45.
Zurück zum Zitat Manteifel VM, Karu TI (2005) Structure of mitochondria and activity of their respiratory chain in successive generations of yeast cells exposed to He-Ne laser light. Izv Akad Nauk Ser Biol 32:556–566 Manteifel VM, Karu TI (2005) Structure of mitochondria and activity of their respiratory chain in successive generations of yeast cells exposed to He-Ne laser light. Izv Akad Nauk Ser Biol 32:556–566
47.
48.
Zurück zum Zitat Woodruff LD, Bounkeo JM, Brannon WM, Dawes KS, Barham CD, Waddell DL, Enwemeka CS (2004) The efficacy of laser therapy in wound repair: a meta-analysis of the literature. Photomed Laser Surg 22:241–247. doi:10.1089/1549541041438623 CrossRefPubMed Woodruff LD, Bounkeo JM, Brannon WM, Dawes KS, Barham CD, Waddell DL, Enwemeka CS (2004) The efficacy of laser therapy in wound repair: a meta-analysis of the literature. Photomed Laser Surg 22:241–247. doi:10.​1089/​1549541041438623​ CrossRefPubMed
49.
Zurück zum Zitat Vladimirov YA, Osipov AN, Klebanov GI (2004) Photobiological principles of therapeutic applications of laser radiation. Biochemistry 69:89–90 Vladimirov YA, Osipov AN, Klebanov GI (2004) Photobiological principles of therapeutic applications of laser radiation. Biochemistry 69:89–90
52.
Zurück zum Zitat Montoya GJV, Ariza J, Sutachán JJ, Hurtado H (2002) Relationship between functional deficiencies and the contribution of myelin nerve fibers derived from L-4, L-5, and L-6 spinolumbar branches in adult rat sciatic nerve. Exp Neurol 173:266–274. doi:10.1006/exnr.2001.7806 CrossRef Montoya GJV, Ariza J, Sutachán JJ, Hurtado H (2002) Relationship between functional deficiencies and the contribution of myelin nerve fibers derived from L-4, L-5, and L-6 spinolumbar branches in adult rat sciatic nerve. Exp Neurol 173:266–274. doi:10.​1006/​exnr.​2001.​7806 CrossRef
Metadaten
Titel
Influence of laser (660 nm) on functional recovery of the sciatic nerve in rats following crushing lesion
verfasst von
Ana Carulina Guimarães Belchior
Filipe Abdalla dos Reis
Renata Amadei Nicolau
Iandara Schettert Silva
Daniel M. Perreira
Paulo de Tarso Camillo de Carvalho
Publikationsdatum
01.11.2009
Verlag
Springer-Verlag
Erschienen in
Lasers in Medical Science / Ausgabe 6/2009
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI
https://doi.org/10.1007/s10103-008-0642-3

Weitere Artikel der Ausgabe 6/2009

Lasers in Medical Science 6/2009 Zur Ausgabe