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01.08.2015 | Original Article

Assessing the biochemical changes of tendons of rats in an experimental model of tenotomy under therapeutic ultrasound and LEDs (625 and 945 nm) by near-infrared Raman spectroscopy

verfasst von: Manoel de Jesus Moura Júnior, Antonio Luís Martins Maia Filho, Diego Rodrigues Pessoa, Marcos Danilo Rodrigues Alves, Jefferson de Sousa Justino, Matheus dos Santos Andrade, Ana Maria Gonçalves Rebêlo, Carlos José de Lima, Antonio Luiz Barbosa Pinheiro, Landulfo Silveira Jr.

Erschienen in: Lasers in Medical Science | Ausgabe 6/2015

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Abstract

Given the complexity of the tendon repair process, where the neoformed scar tissue tends to prevent or hamper the tendon from performing its normal functions, ultrasound (US) and light-emitting diode (LED) devices have been applied to modulate the tendon repair process. This study aimed to compare the anti-inflammatory and healing effects of therapeutic US and LED in an experimental model of tenotomy using histomorphometry and Raman spectroscopy. Forty Wistar rats (235 ± 8.3 g) were separated into four groups: tenotomy (TC; control), tenotomy associated with ultrasound (US; 1 MHz, 0.5 W/cm²), tenotomy associated with LED at 625 nm (LR; 4 J/cm²), and tenotomy associated with LED at 945 nm (LIR; 4 J/cm²), in two experimental periods of 7 and 14 days. Histomorphometry showed a decrease of inflammatory cells (macrophages and polymorphonuclear leukocytes) for the group treated with US compared to the TC group (ANOVA, p < 0.0001) and the LIR group compared to the TC group (p < 0.05) at the seventh day, indicating an anti-inflammatory effect. In relation to the LR and TC groups, there was no significant difference. Raman spectroscopy revealed that the LR group presented an increased synthesis of collagen I compared to the other treatments and the TC groups on the 14th day (p < 0.01). US therapy showed an adjuvant effect with anti-inflammatory action, and the LED therapy at 625 nm showed an increase in collagen synthesis, contributing to the process of tendon healing.

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Titel: Assessing the biochemical changes of tendons of rats in an experimental model of tenotomy under therapeutic ultrasound and LEDs (625 and 945 nm) by near-infrared Raman spectroscopy
verfasst von: Manoel de Jesus Moura Júnior
Antonio Luís Martins Maia Filho
Diego Rodrigues Pessoa
Marcos Danilo Rodrigues Alves
Jefferson de Sousa Justino
Matheus dos Santos Andrade
Ana Maria Gonçalves Rebêlo
Carlos José de Lima
Antonio Luiz Barbosa Pinheiro
Landulfo Silveira Jr.
Publikationsdatum: 01.08.2015
Verlag: Springer London
Erschienen in: Lasers in Medical Science / Ausgabe 6/2015
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-015-1779-5

Springer Medizin

Abstract

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