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26.12.2017 | Original Article

Photobiomodulation increases mitochondrial citrate synthase activity in rats submitted to aerobic training

verfasst von: Wouber Hérickson de Brito Vieira, Cleber Ferraresi, Maria Luiza Barcellos Schwantes, Sérgio Eduardo de Andrade Perez, Vilmar Baldissera, Mikhail Santos Cerqueira, Nivaldo Antonio Parizotto

Erschienen in: Lasers in Medical Science | Ausgabe 4/2018

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Abstract

This study investigated the effects of photobiomodulation by low-laser laser therapy (LLLT) on the activities of citrate synthase (CS) and lactate dehydrogenase (LDH) and the anaerobic threshold (AT) in rats submitted to treadmill exercise. Fifty-four rats were allocated into four groups: rest control (RCG), rest laser (RLG), exercise control (ECG), and exercise laser (ELG). The infrared LLLT was applied daily on the quadriceps, gluteus maximum, soleus, and tibialis anterior muscles. Muscle samples (soleus, tibialis anterior, and cardiac muscles) were removed 48 h after the last exercise session for spectrophotometric analysis of the CS and LDH. The CS activity (μmol/protein) in ELG (16.02 and 0.49) was significantly greater (P < 0.05) than RCG (2.34 and 0.24), RLG (6.25 and 0.17), and ECG (6.76 and 0.26) in the cardiac and soleus muscles, respectively. The LDH activity (in 1 Mm/protein) in soleus muscle was smaller (P < 0.05) for ELG (0.33) compared to ECG (0.97), RLG (0.79), and RCG (1.07). For cardiac muscle, the LDH activity was smaller (P < 0.05) in ELG (1.38) compared to ECG (1.91) and RCG (2.55). The ECG and ELG showed increases in the maximum speed and a shift of the AT to higher effort levels after the training period, but no differences occurred between the exercised groups. In conclusion, the aerobic treadmill training combined with LLLT promotes an increase of oxidative capacity in this rat model, mainly in muscles with greater aerobic capacity.

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Titel: Photobiomodulation increases mitochondrial citrate synthase activity in rats submitted to aerobic training
verfasst von: Wouber Hérickson de Brito Vieira
Cleber Ferraresi
Maria Luiza Barcellos Schwantes
Sérgio Eduardo de Andrade Perez
Vilmar Baldissera
Mikhail Santos Cerqueira
Nivaldo Antonio Parizotto
Publikationsdatum: 26.12.2017
Verlag: Springer London
Erschienen in: Lasers in Medical Science / Ausgabe 4/2018
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-017-2424-2

Springer Medizin

Abstract

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