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Journal of the Association for Research in Otolaryngology

Erschienen in:

01.12.2011

Serial Analysis of Gene Expression in the Chicken Otocyst

verfasst von: Saku T. Sinkkonen, Veronika Starlinger, Deepa J. Galaiya, Roman D. Laske, Samuel Myllykangas, Kazuo Oshima, Stefan Heller

Erschienen in: Journal of the Association for Research in Otolaryngology | Ausgabe 6/2011

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Abstract

The inner ear arises from multipotent placodal precursors that are gradually committed to the otic fate and further differentiate into all inner ear cell types, with the exception of a few immigrating neural crest-derived cells. The otocyst plays a pivotal role during inner ear development: otic progenitor cells sub-compartmentalize into non-sensory and prosensory domains, giving rise to individual vestibular and auditory organs and their associated ganglia. The genes and pathways underlying this progressive subdivision and differentiation process are not entirely known. The goal of this study was to identify a comprehensive set of genes expressed in the chicken otocyst using the serial analysis of gene expression (SAGE) method. Our analysis revealed several hundred transcriptional regulators, potential signaling proteins, and receptors. We identified a substantial collection of genes that were previously known in the context of inner ear development, but we also found many new candidate genes, such as SOX4, SOX5, SOX7, SOX8, SOX11, and SOX18, which previously were not known to be expressed in the developing inner ear. Despite its limitation of not being all-inclusive, the generated otocyst SAGE library is a practical bioinformatics tool to study otocyst gene expression and to identify candidate genes for developmental studies.

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Titel: Serial Analysis of Gene Expression in the Chicken Otocyst
verfasst von: Saku T. Sinkkonen
Veronika Starlinger
Deepa J. Galaiya
Roman D. Laske
Samuel Myllykangas
Kazuo Oshima
Stefan Heller
Publikationsdatum: 01.12.2011
Verlag: Springer-Verlag
Erschienen in: Journal of the Association for Research in Otolaryngology / Ausgabe 6/2011
Print ISSN: 1525-3961
Elektronische ISSN: 1438-7573
DOI: https://doi.org/10.1007/s10162-011-0286-z

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Serial Analysis of Gene Expression in the Chicken Otocyst

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