Skip to main content
Erschienen in: Journal of Assisted Reproduction and Genetics 2-3/2010

01.02.2010 | ANDROLOGY

Spontaneously developed tail swellings (SDTS) influence the accuracy of the hypo-osmotic swelling test (HOS-test) in determining membrane integrity and viability of human spermatozoa

verfasst von: Amjad Hossain, Collin Osuamkpe, Shaikat Hossain, John Y. Phelps

Erschienen in: Journal of Assisted Reproduction and Genetics | Ausgabe 2-3/2010

Einloggen, um Zugang zu erhalten

Abstract

Purpose

To investigate the prevalence of spontaneously developed tail swellings (SDTS) in human sperm samples that are commonly encountered in the laboratory, and their influence on the hypo-osmotic swelling test (HOS-test).

Methods

Ejaculated, epididymal, and testicular sperm were evaluated for SDTS. Further, HOS-test scores were compared with those of vital stains using column washed sperm maintained in the laboratory.

Results

SDTS, at <10%, was present in all types of sperm samples. The highest and lowest occurrences of SDTS were found in cryopreserved sperm, and column-washed sperm respectively. SDTS can inflate the HOS-test score, and so lower the accuracy of the HOS-test. However, the HOS-test efficiency can be improved by assessing SDTS in the sample.

Conclusion

HOS-test and vital stain cannot be used interchangeably in all circumstances for sperm viability determination. The accuracy of the HOS-test can be enhanced by incorporating SDTS as a correction factor.
Literatur
1.
Zurück zum Zitat Jeyendran RS, Van der Ven HH, Perez-Pelaez M, Crabo BG, Zaneveld LJ. Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics. J Reprod Fertil. 1984;70:219–28.PubMedCrossRef Jeyendran RS, Van der Ven HH, Perez-Pelaez M, Crabo BG, Zaneveld LJ. Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics. J Reprod Fertil. 1984;70:219–28.PubMedCrossRef
2.
Zurück zum Zitat Schrader SM, Platek SF, Zaneveld LJ, Perez-Pelaez M, Jeyendran RS. Sperm viability: a comparison of analytical methods. Andrologia. 1986;18:530–8.PubMed Schrader SM, Platek SF, Zaneveld LJ, Perez-Pelaez M, Jeyendran RS. Sperm viability: a comparison of analytical methods. Andrologia. 1986;18:530–8.PubMed
3.
Zurück zum Zitat Munuce MJ, Caille AM, Perfumo P, Morisoli L. Does the hypo-osmotic swelling test predict human sperm viability? Arch Androl. 2000;44:207–12.CrossRefPubMed Munuce MJ, Caille AM, Perfumo P, Morisoli L. Does the hypo-osmotic swelling test predict human sperm viability? Arch Androl. 2000;44:207–12.CrossRefPubMed
4.
Zurück zum Zitat Desmet B, Joris H, Nagy Z, Liu J, Bocken G, Vankelecom A, et al. Selection of vital immotile spermatozoa for intracytoplasmic injection by the hypo-osmotic swelling test. 10th Annual Meeting of ESHRE, June 1994. Hum Reprod. 1994;9(Suppl. 4): p. 24. Desmet B, Joris H, Nagy Z, Liu J, Bocken G, Vankelecom A, et al. Selection of vital immotile spermatozoa for intracytoplasmic injection by the hypo-osmotic swelling test. 10th Annual Meeting of ESHRE, June 1994. Hum Reprod. 1994;9(Suppl. 4): p. 24.
5.
Zurück zum Zitat Casper RF, Cowan L, Lucato ML, Jarvi KA. The hypoosmotic swelling test for the selection of viable sperm for inctracytoplasmic sperm injection in men with complete asthenozoospermia. Fertil Steril. 1996;65:972–6.PubMed Casper RF, Cowan L, Lucato ML, Jarvi KA. The hypoosmotic swelling test for the selection of viable sperm for inctracytoplasmic sperm injection in men with complete asthenozoospermia. Fertil Steril. 1996;65:972–6.PubMed
6.
Zurück zum Zitat Liu J, Tsai YL, Katz E, Compton G, Garcia JE, Baramki TA. High fertilization rate obtained after intracytoplasmic sperm injection with 100% non-motile spermatozoa selected by using a simple modified hypo-osmotic swelling test. Fertil Steril. 1997;68:373–5.CrossRefPubMed Liu J, Tsai YL, Katz E, Compton G, Garcia JE, Baramki TA. High fertilization rate obtained after intracytoplasmic sperm injection with 100% non-motile spermatozoa selected by using a simple modified hypo-osmotic swelling test. Fertil Steril. 1997;68:373–5.CrossRefPubMed
7.
Zurück zum Zitat Smikle CB, Turek PJ. Hypo-osmotic swelling can accurately assess the viability of nonmotile sperm. Mol Reprod Develop. 1997;47:200–3.CrossRef Smikle CB, Turek PJ. Hypo-osmotic swelling can accurately assess the viability of nonmotile sperm. Mol Reprod Develop. 1997;47:200–3.CrossRef
8.
Zurück zum Zitat Lin MH, Morshedi M, Srisombut C, Nassar A, Oehninger S. Plasma membrane integrity of cryopreserved human sperm: an investigation of the results of the hypoosmotic swelling test, the water tests, and eosin-Y staining. Fertil Steril. 1998;70:1148–55.CrossRefPubMed Lin MH, Morshedi M, Srisombut C, Nassar A, Oehninger S. Plasma membrane integrity of cryopreserved human sperm: an investigation of the results of the hypoosmotic swelling test, the water tests, and eosin-Y staining. Fertil Steril. 1998;70:1148–55.CrossRefPubMed
9.
Zurück zum Zitat Jager S, Kremer J, Mijchman J. Hypoosmotic sperm swelling test does not assess fertilizing capacity of human spermatozoa. Arch Androl. 1991;26:195–7.CrossRefPubMed Jager S, Kremer J, Mijchman J. Hypoosmotic sperm swelling test does not assess fertilizing capacity of human spermatozoa. Arch Androl. 1991;26:195–7.CrossRefPubMed
10.
Zurück zum Zitat Esteves SC, Sharma RK, Thomas AJ, Agarwal A. Suitability of the hypo-osmotic swelling test for assessing the viability of cryopreserved sperm. Fertil Steril. 1996;66:798–804.PubMed Esteves SC, Sharma RK, Thomas AJ, Agarwal A. Suitability of the hypo-osmotic swelling test for assessing the viability of cryopreserved sperm. Fertil Steril. 1996;66:798–804.PubMed
11.
Zurück zum Zitat Avery S, Bolton VN, Mason BA. An evaluation of the hypo-osmotic sperm swelling test as a predictor of fertilizing capacity in vitro. Int J Androl. 1990;13:93–9.CrossRefPubMed Avery S, Bolton VN, Mason BA. An evaluation of the hypo-osmotic sperm swelling test as a predictor of fertilizing capacity in vitro. Int J Androl. 1990;13:93–9.CrossRefPubMed
12.
Zurück zum Zitat Martini AC, Estofan D, Ruiz R, Cuneo M. Improving the predictive value of the hypo-osmotic swelling test in humans. Fertil Steril. 2006;85:1840–2.CrossRefPubMed Martini AC, Estofan D, Ruiz R, Cuneo M. Improving the predictive value of the hypo-osmotic swelling test in humans. Fertil Steril. 2006;85:1840–2.CrossRefPubMed
13.
Zurück zum Zitat World Health Organization. WHO laboratory manual for the examination of human semen and sperm-cervical mucus interaction. 4th ed. Cambridge: Cambridge University; 1999. World Health Organization. WHO laboratory manual for the examination of human semen and sperm-cervical mucus interaction. 4th ed. Cambridge: Cambridge University; 1999.
14.
Zurück zum Zitat Gardner DK, Weissman A, Howles CM, Shoham Z. Textbook of assisted reproductive techniques: laboratory and clinical perspectives. 1st ed. London: Dunitz; 2001. Gardner DK, Weissman A, Howles CM, Shoham Z. Textbook of assisted reproductive techniques: laboratory and clinical perspectives. 1st ed. London: Dunitz; 2001.
15.
Zurück zum Zitat Rossato M, Balercia G, Lucarelli G, Mantero F. Role of seminal osmolarity in the regulation of human sperm motility. Int J Androl. 2002;37:207–18. Rossato M, Balercia G, Lucarelli G, Mantero F. Role of seminal osmolarity in the regulation of human sperm motility. Int J Androl. 2002;37:207–18.
16.
Zurück zum Zitat Hossain A, Osuamkpe C, Nagamani M. Extended culture of human spermatozoa in the laboratory may have practical value in the assisted reproductive procedures. Fertil Steril. 2007;89:237–9.CrossRefPubMed Hossain A, Osuamkpe C, Nagamani M. Extended culture of human spermatozoa in the laboratory may have practical value in the assisted reproductive procedures. Fertil Steril. 2007;89:237–9.CrossRefPubMed
17.
Zurück zum Zitat Misro M, Chaki S. Development of a rapid, sensitive, and reproducible laboratory test kit for the assessment of plasma membrane integrity of human sperm. Fertil Steril. 2008;89:223–7.CrossRefPubMed Misro M, Chaki S. Development of a rapid, sensitive, and reproducible laboratory test kit for the assessment of plasma membrane integrity of human sperm. Fertil Steril. 2008;89:223–7.CrossRefPubMed
Metadaten
Titel
Spontaneously developed tail swellings (SDTS) influence the accuracy of the hypo-osmotic swelling test (HOS-test) in determining membrane integrity and viability of human spermatozoa
verfasst von
Amjad Hossain
Collin Osuamkpe
Shaikat Hossain
John Y. Phelps
Publikationsdatum
01.02.2010
Verlag
Springer US
Erschienen in
Journal of Assisted Reproduction and Genetics / Ausgabe 2-3/2010
Print ISSN: 1058-0468
Elektronische ISSN: 1573-7330
DOI
https://doi.org/10.1007/s10815-009-9375-x

Weitere Artikel der Ausgabe 2-3/2010

Journal of Assisted Reproduction and Genetics 2-3/2010 Zur Ausgabe

Ambulantisierung: Erste Erfahrungen mit dem Hybrid-DRG

02.05.2024 DCK 2024 Kongressbericht

Die Hybrid-DRG-Verordnung soll dazu führen, dass mehr chirurgische Eingriffe ambulant durchgeführt werden, wie es in anderen Ländern schon länger üblich ist. Die gleiche Vergütung im ambulanten und stationären Sektor hatten Niedergelassene schon lange gefordert. Aber die Umsetzung bereitet ihnen doch Kopfzerbrechen.

Sind Frauen die fähigeren Ärzte?

30.04.2024 Gendermedizin Nachrichten

Patienten, die von Ärztinnen behandelt werden, dürfen offenbar auf bessere Therapieergebnisse hoffen als Patienten von Ärzten. Besonders gilt das offenbar für weibliche Kranke, wie eine Studie zeigt.

Harninkontinenz: Netz-Op. erfordert über lange Zeit intensive Nachsorge

30.04.2024 Harninkontinenz Nachrichten

Frauen mit Belastungsinkontinenz oder Organprolaps sind nach einer Netz-Operation keineswegs beschwerdefrei. Vielmehr scheint die Krankheitslast weiterhin hoch zu sein, sogar höher als von harninkontinenten Frauen, die sich nicht haben operieren lassen.

Welche Übungen helfen gegen Diastase recti abdominis?

30.04.2024 Schwangerenvorsorge Nachrichten

Die Autorinnen und Autoren einer aktuellen Studie aus Griechenland sind sich einig, dass Bewegungstherapie, einschließlich Übungen zur Stärkung der Bauchmuskulatur und zur Stabilisierung des Rumpfes, eine Diastase recti abdominis postpartum wirksam reduzieren kann. Doch vieles ist noch nicht eindeutig belegt.

Update Gynäkologie

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert – ganz bequem per eMail.