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Journal of Mammary Gland Biology and Neoplasia

Erschienen in:

01.06.2012

Isolation of Mouse Mammary Epithelial Subpopulations: A Comparison of Leading Methods

verfasst von: Matthew J. Smalley, Howard Kendrick, Julie M. Sheridan, Joseph L. Regan, Michael D. Prater, Geoffrey J. Lindeman, Christine J. Watson, Jane E. Visvader, John Stingl

Erschienen in: Journal of Mammary Gland Biology and Neoplasia | Ausgabe 2/2012

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Abstract

Isolation of mammary epithelial subpopulations, including stem and progenitor cells, has become a standard technique in recent years. However, a number of methods and approaches for this have developed and the relative benefits of the different approaches, and the reason for their development, have not always been clear. Here, three of the leading laboratories working on the separation of mammary cell subpopulations have summarised their methods, highlighted their differences and similarities and also discussed the reasoning behind the approaches they have taken. This article will assist workers establishing mammary cell separation protocols in their laboratories to make informed choices about the methods they should use.

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Titel: Isolation of Mouse Mammary Epithelial Subpopulations: A Comparison of Leading Methods
verfasst von: Matthew J. Smalley
Howard Kendrick
Julie M. Sheridan
Joseph L. Regan
Michael D. Prater
Geoffrey J. Lindeman
Christine J. Watson
Jane E. Visvader
John Stingl
Publikationsdatum: 01.06.2012
Verlag: Springer US
Erschienen in: Journal of Mammary Gland Biology and Neoplasia / Ausgabe 2/2012
Print ISSN: 1083-3021
Elektronische ISSN: 1573-7039
DOI: https://doi.org/10.1007/s10911-012-9257-1

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Isolation of Mouse Mammary Epithelial Subpopulations: A Comparison of Leading Methods

Abstract

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