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01.04.2013 | Research Article

Calreticulin expression in infiltrating ductal breast carcinomas: relationships with disease progression and humoral immune responses

verfasst von: Maria Kabbage, Mounir Trimeche, Sarra Bergaoui, Philippe Hammann, Lauriane Kuhn, Bechr Hamrita, Hela ben Nasr, Anouar Chaieb, Lotfi Chouchane, Karim Chahed

Erschienen in: Tumor Biology | Ausgabe 2/2013

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Abstract

The aim of this study was to evaluate calreticulin expression in infiltrating ductal breast carcinomas (IDCAs), as well as its relationships with clinicopathological parameters of the disease. Using a two-dimensional gel electrophoresis/matrix-assisted laser desorption ionization time of flight mass spectrometry investigation coupled to an immunohistochemical approach, we have assessed the expression of calreticulin in IDCAs, as well as in other types of breast tumors. The humoral immune response against calreticulin was estimated using a serological proteomics-based strategy. Proteomic analyses revealed an increased expression of calreticulin in IDCA tumors. Using immunohistochemistry, overexpression of calreticulin was confirmed in 51 additional tumor specimens. Statistical analyses revealed, however, no significant correlations between calreticulin expression and clinicopathological parameters of the disease including tumor stage, patient age, SBR grade, and lymph node metastasis occurrence. A significant association was found, however, with estrogen receptor status. This study demonstrates the upregulation of calreticulin in IDCA tissues which may highlight its involvement in breast cancer development. Our findings also support a link between calreticulin expression and estrogen transduction pathways. Our results do not, however, support the involvement of calreticulin in the development of a humoral immune response in IDCAs.

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Titel: Calreticulin expression in infiltrating ductal breast carcinomas: relationships with disease progression and humoral immune responses
verfasst von: Maria Kabbage
Mounir Trimeche
Sarra Bergaoui
Philippe Hammann
Lauriane Kuhn
Bechr Hamrita
Hela ben Nasr
Anouar Chaieb
Lotfi Chouchane
Karim Chahed
Publikationsdatum: 01.04.2013
Verlag: Springer Netherlands
Erschienen in: Tumor Biology / Ausgabe 2/2013
Print ISSN: 1010-4283
Elektronische ISSN: 1423-0380
DOI: https://doi.org/10.1007/s13277-013-0661-y

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Calreticulin expression in infiltrating ductal breast carcinomas: relationships with disease progression and humoral immune responses

Abstract

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