nach oben

Tumor Biology

Erschienen in:

01.10.2014 | Research Article

RETRACTED ARTICLE: MicroRNA-32 inhibits osteosarcoma cell proliferation and invasion by targeting Sox9

verfasst von: Jian-Qiang Xu, Wei-Bin Zhang, Rong Wan, Yao-Qi Yang

Erschienen in: Tumor Biology | Ausgabe 10/2014

Einloggen, um Zugang zu erhalten

Abstract

Increasing reports suggest that discovery of microRNAs (miRNAs) might provide a novel therapeutical target for human cancers, including osteosarcoma. Previous studies have shown that miR-32 was dysregulated in breast and endometrial cancer. However, its biological roles in osteosarcoma remain unclear. In the current study, we found that miR-32 was significantly down-regulated in osteosarcoma tissues, compared with the adjacent normal tissues. In vitro studies further demonstrated that miR-32 mimics were able to suppress, while its antisense oligos promoted cell proliferation in Saos-2 and U2OS cells. At the molecular level, our data further revealed that expression of Sox9 was negatively regulated by miR-32. Therefore, our results identify an important role for miR-32 in the osteosarcoma through regulating Sox9 expression.

Nur mit Berechtigung zugänglich

Luetke A, Meyers PA, Lewis I, Juergens H. Osteosarcoma treatment—where do we stand? A state of the art review. Cancer Treat Rev. 2014;40(4):523–32.CrossRefPubMed

Botter SM, Neri D, Fuchs B. Recent advances in osteosarcoma. Curr Opin Pharmacol. 2014;16C:15–23.CrossRef

Sun K, Lai EC. Adult-specific functions of animal microRNAs. Nat Rev Genet. 2013;14:535–48.CrossRefPubMedPubMedCentral

Ameres SL, Zamore PD. Diversifying microRNA sequence and function. Nat Rev Mol Cell Biol. 2013;14:475–88.CrossRefPubMed

Miao J, Wu S, Peng Z, et al. MicroRNAs in osteosarcoma: diagnostic and therapeutic aspects. Tumour Biol. 2013;34(4):2093–8.CrossRefPubMed

Nugent M. MicroRNA function and dysregulation in bone tumors: the evidence to date. Cancer Manag Res. 2014;6:15–25.CrossRefPubMedPubMedCentral

Wang Z, Cai H, Lin L, et al. Upregulated expression of microRNA-214 is linked to tumor progression and adverse prognosis in pediatric osteosarcoma. Pediatr Blood Cancer. 2014;61(2):206–10.CrossRefPubMed

Ambs S, Prueitt RL, Yi M, et al. Genomic profiling of microRNA and messenger RNA reveals deregulated microRNA expression in prostate cancer. Cancer Res. 2008;68(15):6162–70.CrossRefPubMedPubMedCentral

Wu W, Yang J, Feng X, et al. MicroRNA-32 (miR-32) regulates phosphatase and tensin homologue (PTEN) expression and promotes growth, migration, and invasion in colorectal carcinoma cells. Mol Cancer. 2013;12:30.CrossRefPubMedPubMedCentral

10.

Wu W, Yang P, Feng X, et al. The relationship between and clinical significance of MicroRNA-32 and phosphatase and tensin homologue expression in colorectal cancer. Gene Chromosome Cancer. 2013;52(12):1133–40.CrossRef

11.

Zhang J, Kuai X, Song M, et al. microRNA-32 inhibits the proliferation and invasion of the SGC-7901 gastric cancer cell line in vitro. Oncol Lett. 2014;7(1):270–4.PubMed

12.

Weber B, Stresemann C, Brueckner B, Lyko F. Methylation of human microRNA genes in normal and neoplastic cells. Cell Cycle. 2007;6(9):1001–5.CrossRefPubMed

13.

Suzuki H, Maruyama R, Yamamoto E, Kai M. DNA methylation and microRNA dysregulation in cancer. Mol Oncol. 2012;6(6):567–78.CrossRefPubMed

14.

Panda DK, Miao D, Lefebvre V, et al. The transcription factor SOX9 regulates cell cycle and differentiation genes in chondrocytic CFK2 cells. J Biol Chem. 2001;276(44):41229–36.CrossRefPubMed

15.

Saegusa M, Hashimura M, Suzuki E, et al. Transcriptional up-regulation of Sox9 by NF-κB in endometrial carcinoma cells, modulating cell proliferation through alteration in the p14(ARF)/p53/p21(WAF1) pathway. Am J Pathol. 2012;181(2):684–92.CrossRefPubMed

16.

Wegner M. All purpose Sox: the many roles of Sox proteins in gene expression. Int J Biochem Cell Biol. 2009;42:381–90.CrossRefPubMed

17.

Matheu A, Collado M, Wise C, et al. Oncogenicity of the developmental transcription factor Sox9. Cancer Res. 2012;72(5):1301–15.CrossRefPubMedPubMedCentral

18.

Akiyama H, Chaboissier MC, Martin JF, et al. The transcription factor Sox9 has essential roles in successive steps of the chondrocyte differentiation pathway and is required for expression of Sox5 and Sox6. Genes Dev. 2002;16:2813–28.CrossRefPubMedPubMedCentral

19.

Kordes U, Hagel C. Expression of SOX9 and SOX10 in central neuroepithelial tumor. J Neurooncol. 2006;80:151–5.CrossRefPubMed

20.

Jiang SS, Fang WT, Hou YH, et al. Upregulation of SOX9 in lung adenocarcinoma and its involvement in the regulation of cell growth and tumorigenicity. Clin Cancer Res. 2010;16:4363–73.CrossRefPubMed

21.

Wang H, Leav I, Ibaragi S, et al. SOX9 is expressed in human fetal prostate epithelium and enhances prostate cancer invasion. Cancer Res. 2008;68:1625–30.CrossRefPubMed

22.

Bastide P, Darido C, Pannequin J, et al. Sox9 regulates cell proliferation and is required for Paneth cell differentiation in the intestinal epithelium. J Cell Biol. 2007;178(4):635–48.CrossRefPubMedPubMedCentral

23.

Cheung M, Chaboissier MC, Mynett A, et al. The transcriptional control of trunk neural crest induction, survival, and delamination. Dev Cell. 2005;8:179–92.CrossRefPubMed

24.

Zhu H, Tang J, Tang M, Cai H. Upregulation of SOX9 in osteosarcoma and its association with tumor progression and patients’ prognosis. Diagn Pathol. 2013;8(1):183.CrossRefPubMedPubMedCentral

25.

Rani SB, Rathod SS, Karthik S, et al. MiR-145 functions as a tumor-suppressive RNA by targeting Sox9 and adducin 3 in human glioma cells. Neuro Oncol. 2013;15(10):1302–16.CrossRefPubMedPubMedCentral

26.

Lee S, Yoon DS, Paik S, et al. MicroRNA-495 inhibits chondrogenic differentiation in human mesenchymal stem cells by targeting Sox9. Stem Cells Dev. 2014 Mar 24.

Titel: RETRACTED ARTICLE: MicroRNA-32 inhibits osteosarcoma cell proliferation and invasion by targeting Sox9
verfasst von: Jian-Qiang Xu
Wei-Bin Zhang
Rong Wan
Yao-Qi Yang
Publikationsdatum: 01.10.2014
Verlag: Springer Netherlands
Erschienen in: Tumor Biology / Ausgabe 10/2014
Print ISSN: 1010-4283
Elektronische ISSN: 1423-0380
DOI: https://doi.org/10.1007/s13277-014-2229-x

Springer Medizin

RETRACTED ARTICLE: MicroRNA-32 inhibits osteosarcoma cell proliferation and invasion by targeting Sox9

Abstract

Neu im Fachgebiet Onkologie

Adjuvante Immuntherapie verlängert Leben bei RCC

Alectinib verbessert krankheitsfreies Überleben bei ALK-positivem NSCLC

Bei Senioren mit Prostatakarzinom auf Anämie achten!

ICI-Therapie in der Schwangerschaft wird gut toleriert

Update Onkologie

Springer Medizin

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 10/2014

Identification of circulating microRNAs as biomarkers in diagnosis of hematologic cancers: a meta-analysis

Expression of metabolism-related proteins in invasive lobular carcinoma: comparison to invasive ductal carcinoma

BTG1 underexpression is an independent prognostic marker in esophageal squamous cell carcinoma

Role of epithelial to mesenchymal transition proteins in gynecological cancers: pathological and therapeutic perspectives

Association between TNF-α gene 308G>A polymorphism and lung cancer risk: a meta-analysis

MiR-7-5p is frequently downregulated in glioblastoma microvasculature and inhibits vascular endothelial cell proliferation by targeting RAF1

Neu im Fachgebiet Onkologie

Adjuvante Immuntherapie verlängert Leben bei RCC

Alectinib verbessert krankheitsfreies Überleben bei ALK-positivem NSCLC

Bei Senioren mit Prostatakarzinom auf Anämie achten!

ICI-Therapie in der Schwangerschaft wird gut toleriert

Update Onkologie