Methods
Study design and data collection
The study was carried out in the Municipality of Salamis located in the island with the same name, which belongs to the greater region of Attica, at a relatively small distance from Athens.
The survey was conducted during municipal elections, on 13th October 2002. Ten specifically trained health professional interviewers performed face-to-face interviews, using a standardized, previously validated (by our institution) questionnaire. The questionnaire was anonymous, and took no more than 3 minutes to complete. All Salamis poll stations, 8 in total, were included in the sampling and individuals were selected at random on attendance. The participants were unaware about the content of questionnaire, in order to minimize affecting reported data by selection bias.
The following information was collected from the participants concerning: year of birth, sex, origin (born or not in Salamis), self reported height and weight, educational level (low or ≤ 9 years of school, moderate or 10–14 years of school and higher or >14 years of school), current or former smoking, as well as medical history of diabetes and hypertension, as evaluated by the interviewer.
Individuals were considered to have diabetes if they reported a previous medical diagnosis of diabetes. They were also asked if they were using insulin or oral antidiabetic agents. Presence of diabetes was confirmed by question regarding the year (or age) at diagnosis of diabetes. Family history of diabetes (in parents or/and siblings) was recorded.
The subjects were considered as hypertensive if they had been previously diagnosed and were also asked if they were using antihypertensive drugs. We also retrieved information regarding history of myocardial infarction and known hypercholesterolemia.
The smoking habit was categorized as: smokers if they were currently smoking ≥ 5 cigarettes per day, non-smokers if never smoked and former smokers if they had smoked previously but were nonsmokers at least one year before the interview.
We used data on self reported weight and height to calculate BMI. Participants were classified as overweight if their BMI was 25–29.99 kg/m2 and as obese if BMI was ≥ 30 kg/m2.
The study protocol was approved by the Scientific and Ethical Committee of the "Saint Panteleimon" General Hospital of Nicea.
The population of the study
The sample consisted of 2805 adults, aged 20–94 years old. Of them, 1375 were men (mean age 49.3 ± 17.3) and 1430 were women (mean age 49.1 ± 17.4). The overall response rate of eligible participants was 91%. Respondents younger than 20 years old were excluded from the study. The age and sex distribution of participants in the survey was compared with Census 2001 data for municipality of Salamis [
11]. As shown in table
1, there were only minor differences in distribution by sex and age between the study and the target population. It seems that, the high participation of attendance (86% of registered voters) to the election contributed considerably to obtain a representative sample.
Table 1
Age and sex stratification of random selected population sample of adult residents in municipality of Salamis, Greece, who participated in survey.
20–29 | 2674 | 246 | 0.092 | 1904 | 267 | 0.140 | 4578 | 513 | 0.112 |
30–39 | 2003 | 229 | 0.114 | 1925 | 254 | 0.132 | 3928 | 483 | 0.123 |
40–49 | 1680 | 237 | 0.141 | 1817 | 229 | 0.126 | 3497 | 466 | 0.133 |
50–59 | 1712 | 222 | 0.130 | 1881 | 227 | 0.121 | 3593 | 449 | 0.125 |
60–69 | 1912 | 240 | 0.126 | 1961 | 231 | 0.120 | 3873 | 471 | 0.122 |
≥70 | 1513 | 201 | 0.133 | 1645 | 222 | 0.135 | 3158 | 423 | 0.134 |
Total | 11494 | 1375 | 0.120 | 11133 | 1430 | 0.128 | 22627 | 2805 | 0.124 |
Statistical analysis
Prevalence estimations were made taking into account the stratified sampling procedure. Group comparisons were performed by the use of Pearson's chi-square test. Multiple logistic regression analysis was applied to determine the associations of established risk factors on the presence of diabetes, calculating odds ratio (OR) and their corresponding 95% confidence intervals (CI). Eight risk factors were tested in the full model: age, sex, education, overweight/obesity, smoking, hypertension, origin and family history of diabetes. Furthermore, a stratified analysis was performed for men and women, respectively. Statistical significance was set at P-value of 0.05. Prevalence of diabetes was age-adjusted by using the direct method based on the 2001 standard population of Greece. SPSS 11 statistical software was used for all statistical calculations (SPSS Inc, Illinois, USA)
Discussion
The present study assessed the current prevalence of known DM in a random sample of urban adult Greek population. To our knowledge, this study is the first that report the prevalence of DM in relation to the most common risk factors such as overweight/obesity, heredity etc, in Greek population.
The sampling, conducted in the elective body of the reference population ensured the representativeness of the sample, for age and sex distribution. The rate of non-participants was relatively low, so the selection bias is considered limited. As an additional strength of this study, could be considered, the finding that the proportions regarding educational level in our sample were similar with those observed for adult Greek population: 52.5% with low education, 31.7% with moderate, 15.8% with higher education and 52.4%, 32.8%, 14.8%, respectively [
11]. What was unavoidable in this study is the information bias regarding the source of the data (self-reported), which is leading to the underestimation of the true prevalence of DM. However, despite the potential influences of self-reporting bias this is a common investigated tool used for diabetes surveillance [
7,
8,
12]. Moreover, it has been found that self-reported diabetes was more accurate than self-reported hypertension or heart disease [
13].
We found an overall crude prevalence of self-reported DM of 8.7%. After age adjustment for the current adult population (2001 census) of Greece, the projection prevalence was calculated to 8.2%. Thus it could be speculated that the prevalence of known DM, in Greek adult population, is about 700 thousands patients. The question that is rising is which might be the actual total number of individuals with diabetes, in Greece, taking into account the proportion of previously undiagnosed diabetes, that are likely to have been missed, by this survey. It is reported that the proportion of previously undiagnosed diabetes worldwide is ranged from 32–66% [
3,
9,
14,
15]. The only available information in Greece is from a study that was conducted 10 years ago in a semi-urban elderly population (n = 581), where an extremely high proportion (65%) of undiagnosed diabetes was found [
16]. Based on the fore-mentioned major discrepancies and lack of current data, it is hard to provide a reliable estimation for the Greek population. Nevertheless, based on the lowest reported prevalence of undiagnosed diabetes (32%), it is estimated that about 1.05 million adults have diabetes (a predicted prevalence of 12%). This is a high diabetes burden for a developed European country.
A prior publication concerning a home survey, in 1990, in municipality of Aegaleo at distance of about 10 km from Salamis, found known diabetes in 5.4% of respondents aged 20 years and older [
10]. The age-standardized prevalence of diabetes in this population was calculated to 5.9% (table
2). Comparing that prevalence to our findings (8.2%), we observed a 39% increase over the 12 year period (3.3% per year). That increasing prevalence of diabetes in Greek population seems to be higher as compared with the rate between years 1974 to 1990 (2.4 to 3.1%, a rate of 1.8% per year) [
10]. These findings suggest that apart from the increase in the prevalence of diabetes, an increased incidence of diabetes probably occurred during the last 12 years. This is in coincidence with a very marked socio-economic evolution that has been experienced in Greece, after 1980s, where the significant improvement of standard livings has dramatically changed lifestyle [
17]. Consequently, the increased diabetes prevalence, and perhaps incidence, could be attributed to the adoption of western culture and lifestyle (specifically the interrelated issues of increasing obesity, decreasing physical activity levels and dietary change) [
18,
19]. However, an increasing prevalence of diabetes does not necessarily implicate an increasing incidence, because the increasing prevalence may reflect better case ascertainment and prolonged survival of diabetic patients as suggested by the findings of Berger et al [
20] and Stovring et al [
21]. Further studies, mainly longitudinal ones, needs to clarify this issue.
Indicative of the increased prevalence of diabetes in Greece, especially in last decade, are the results of the general population-based study in a municipality of rural Crete, in 1999, where a relatively high age standardized (for European population) prevalence of diabetes (5.2%) was found [
22], while the age-standardized prevalence of known diabetes in another area of rural Crete, in 1988–1993, was 1.5% [
23]
In accordance with the current data, our study shows a clear relationship between age and diabetes. Diabetes (type 2) has been considered a disease of middle-aged and elderly. The previous "Aegaleo" studies showed that the increase in diabetes, between 1974 and 1990, begin in those older than 50 years [
10], whereas our data shown clearly that the prevalence is increased considerably in both sexes after the age of 30 years (table
2).
A small dominance of men over women (OR = 1.43) in diabetes prevalence was observed in our study. A similar difference has been observed in other studies, in Australia where the OR = 1.31 [
9], in Canada OR = 1.44 [
11], while others did not reported any sex difference [
1,
3]. The explanation for these sex differences may be that men are more susceptible than women to the consequences of indolence and obesity, possibly due to differences in insulin sensitivity and regional fat deposition [
24].
Previous data showed that prevalence of diabetes (type 2) was inversely related to socioeconomic status [
25]. Among socioeconomic status indicators it has been manifested that income is more strongly associated with diabetes prevalence, especially among women [
12,
26]. In this study, lower educational level was associated with diabetes only among women. Our explanation for this finding is that among Greek female population the educational level might be as strong an indicator for socioeconomic status as income [
27].
So far, there are no clear data indicating smoking as an independent risk factor for diabetes, but for diabetes associated complications such as heart diseases [
28]. In our study no strong association of smoking and DM was found, finding in concordance with other cross-sectional studies [
29]. While the finding that frequency was higher in former men smokers is probably resulting from smoking cessation due to medical advice after development of a macrovascular complication, such as myocardial infarction. However, in contrary to the most of cross-sectional studies there is now evidence from at least five prospective studies to suggest that smoking is associated with increased risk of type 2 diabetes [
19].
In a recent telephone-based study, prevalence of hypertension in adults with DM was 56% vs 22% in those without DM (a difference of 34%) [
30]. Similar were our results showing that prevalence of hypertension in adults with DM was 51% vs 17% (a difference of 34%), confirming the strong relation between hypertension and diabetes. The result that hypertensive women are more likely to have diabetes is interesting and warrants further investigation for confirmation.
It is well known that diabetes is strongly associated with obesity [
18,
31]. Studies in countries with solid information shown that the increase in obesity has been attended strictly by an increase in the prevalence of DM [
7,
9,
32,
33]. There is some evidence that rate of obesity, in Greece, has increased in the last decades. This is attributed in considerable changes in nutrition and adoption of sedentary lifestyle [
34].
We found that the prevalence of either overweight or obesity among Greek men and women was very high: 73.6% and 51.8%, respectively. Bearing in mind that the risk is increased above a BMI of 25, it is understood that the majority of Greek population is exposed to at least 2-fold increased risk for diabetes. Consequently, these findings have alarming implications for population health and health care expenditure over the next years.
Our findings, compared to the data reported from other similar studies (based on self-reported data), lead to the conclusion that the prevalence of overweight/obesity in Greece is close to USA rates, particularly in older adults [
8]. Also, it seems that represents one of the highest obesity rates in Europe [
35], confirming the findings of recent study [
36].
The study confirms clearly that overweight/obesity is the most important modifiable risk factor for diabetes. Moreover, we found that obesity has a stronger effect on the prevalence of diabetes among women than men, which is consistent with other studies [
12]. This finding could have important implications for the prevention of diabetes, because it suggests that targets for the management of obesity should be different: i.e. much stricter for women than men. However, we must be careful in those interpretations, because this study has the disadvantage of being a self-reported data study, where the true prevalence of overweight and obesity is expected to be underestimated by men and women in almost all ages [
37,
38]. In particular, participants are most likely to recall their height as measured in early adulthood and, as height loss is greatest in the elderly, the difference between their recalled and actual height will be greater. In regard with weight, most subjects under-reported their weight, especially the overweighed subjects [
39]. Also, in some studies it was observed that errors in self-reported weight were greater in women than men [
40,
41]. It is interesting to note that, in this situation of underestimations of the true size of overweight/obesity, a correction procedure – locally calibrated BMI values – which will improve the validity of estimations, is recommended [
37,
38].
This study not only confirms the important role of heredity in the prevalence of diabetes, but also gives more evidence for this relationship. As a matter of fact, the effect of first-degree FH of diabetes was found to be substantially higher (7-fold) as compared with other countries [
1,
42,
43], indicated that the contribution of genetic background in diabetes varies by ethnic groups. Moreover, our findings add weight to previous reports that diabetes is more likely to be transmitted from mother than from father [
44,
45].
Although it is recognized that obese people with FH of diabetes are at high risk of developing type 2 diabetes, few population-based studies have quantified the excess risk of the combination of both of them [
46,
47]. In our study, the synergistic effect of overweight with FH of diabetes was 15-fold and of obesity with FH 25-fold higher compared with normal weight and negative heredity. Practically, these findings suggest that public health target is obesity (as the most important modifiable risk factor for DM), focusing on especially high-risk group of positive heredity for diabetes.
Study limitations
Some limitations of this study are the following. 1) Only data on self-reported diabetes were used, therefore, it was likely to underestimate the true prevalence of diabetes. 2) Also, self-reported data were used regarding the BMI, which was not associated with the correction procedure of locally calibrated BMI values, so the underestimation of obesity and overweight distribution in our sample may exist. 3) We did not take into account the physical activity status that may constitute a strong risk factor for diabetes.
Authors' contributions
AG had the original idea for the study, organized the study and contributed to the design, in statistical analyses of the data and writing of the paper. AS contribute to the design, on interpretation of results and preparation of manuscript for publication. DP provided expert advice in study design, data analysis and contributed to the writing of the paper. TP and ES helped to the preparation of the manuscript. SP supervised all phases of the study and manuscript preparation. All authors read and approved the final manuscript