Background
Methods
Preparation and compositional analysis of KJ
Animal, model and treatment
Behavior tests
Cerebral perfusion
The terminal transferase dUTP nick end labeling (TUNEL) assay
Immunohistochemical analysis
Real-time PCR analysis
Gene | Primer | Product length (bp) | |
---|---|---|---|
MMP–2 | F | 5′-ACACCCTCAAGAAGATGCAGA-3′ | 237 |
R | 5′-ATACTTTTAAGGCCCGAGCAA-3′ | ||
MMP–9 | F | 5′-TTTGGAAACGCAAATGGTG-3′ | 210 |
R | 5′-TGGAAATACGCAGGGTTTG-3′ | ||
t–PA | F | 5′-AGACATCACCTCACACCCTTG-3′ | 194 |
R | 5′-TTCCAGGGACCACTCTGTATG-3′ | ||
PAI–1 | F | 5′-GCCCCACTTCTTCAAGCTC-3′ | 160 |
R | 5′-CAGGCGTGTCAGCTCATTT-3′ | ||
BDNF | F | 5′-GCATCAGAAAAAGAGGCAAAC-3′ | 194 |
R | 5′-AGGCTACGTGAAGTCTTCCAA-3′ | ||
TrkB | F | 5′-GTCTGCTGAAGCCTGCATATC-3′ | 191 |
R | 5′-ATGAGTGCGTTGGAATGAAAC-3′ | ||
GAPDH | F | 5′-CCATGGAGAAGGCTGGG3′ | 195 |
R | 5′-CAAAGTTGTCATGGATGACC-3′ |
Western blot analysis
Data analysis
Results
Composition of KJ preparation
Origin of Herb | Compound | Formula | Content (mg/g) |
---|---|---|---|
Morinda officinalis How | Nistose | C24H42O21 | 2.50 |
Paeonia lactiflora Pall. | Paeoniflorin | C23H28O11 | 3.06 |
Bupleurum chinense DC. | Saikosaponin A | C42H68O13 | 0.28 |
Saikosaponin D | C42H68O13 | 0.13 | |
Glycyrrhiza uralensis Fisch. | Glycyrrhizic acid Ammonium salt | C42H65NO16 | 0.95 |
Liquiritin | C21H22O9 | 0.52 | |
Citrus aurantium L. | Naringin | C27H32O14 | 3.72 |
Neohesperidin | C28H34O15 | 1.06 | |
Hesperidin | C28H34O15 | 15.24 | |
Panax ginseng C. A. Mey. | Ginsenoside Rg1 | C42H72O14 | 1.19 |
Ginsenoside Re | C48H82O18 | 1.01 | |
Ginsenoside Rb1 | C54H92O23 | 1.44 |