Background
Materials and methods
HCV genotype | I-PCR primers | II-PCR primers | 5’-3’ sequenceb
| H77 location |
---|---|---|---|---|
1b | MarsF2a
MarsR1a
| NS31b Fb
MarsR2a
| 5'- TRCCHGTCTCCGCCCGVAG-3'c
| 3291-3310 4215–4237 3340–3358 4035-4054 |
2c | MarsF1a
MarsR1a
| NS3 2c Fb
NS3 2c Rb
| 5'- TGGGCCCTGCTGATGGATAC-3'd
5'- GTAGGTCTGGGGCACAGCTG-3' | 3291-3310 4215–4237 3376–3395 4010-3991 |
4d | G4F1a
G4R1a
| NS3 4d Fb
NS3 4d Rb
| 5’-ATGCGCACRCYAYGAAGGG-3’e
5’-GGCACGGCAGGAGGAGTGGA-3’ | 3369-3388 3997–4018 3388–3407 4000-3981 |
Results
Rb
| Pc
| Dd
| Me
| Drug | G 1a (n = 21) | G 1b (n = 21) | G 2a (n = 2) | G 2c (n = 60) | G 3a (n = 20) | G 4d (n = 25) | G 4 k (n = 1) |
---|---|---|---|---|---|---|---|---|---|---|---|
NS5B | 316 | C | N,Y,H | Sofosbuvir, Mericitabine | • |
*C(57)/N(38),H(5) | • | • | • | • | • |
NS5B | 282 | S | T | Sofosbuvir, Mericitabine | • | • | • | • | • | • | • |
NS5B | 320 | I | Sofosbuvir, Mericitabine | • | • | • | • | • | • | • | |
NS5B | 321 | V | Sofosbuvir, Mericitabine | • | • | • | • | • | • | • |
Rb
| Pc
| Dd
| Me
| Drug | G 1b (n = 11) | G 2c (n = 11) | G 4d (n = 6) |
---|---|---|---|---|---|---|---|
NS3 | 16 | C | S | ACH806 | *C/R(9) | *A(100) | *T(100) |
NS3 | 36 | V | A,M,G,L | Telaprevir, Boceprevir | *V(100) | L(100)f
| L(100)f
|
NS3 | 54 | T | A,S | Telaprevir, Boceprevir |
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NS3 | 155 | R | K,T,I,MG,L,S,Q | Telaprevir, Boceprevir |
•
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•
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NS3 | 156 | A | S,T,V,I | Telaprevir, Boceprevir |
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•
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NS3 | 168 | D | Q,A,V,E,T | TMC435,R7227,MK7009, BI201335, BILN2061 |
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NS3 | 175 | M | L | Telaprevir | *M(100) | L(100)f
| L(100)f
|
aPosition |
bSubtype 1b |
aPosition |
bSubtype 1b |
---|---|---|---|
7 | S7/A (6) | 114 | V114/I (11) |
14 | L14/V (1) | 122 | S122/T (1) |
30 | D30/E (10) | 128 | S128/Y (1) |
48 | V48/I (6) | 131 | P131/T (1) |
51 | V51/A (1) | 132 | I132/V/L (5) |
56 | Y56/F (4) | 142 | P142/L (1) |
61 | S61/P/T (5) | 150 | V150/A (9) |
83 | V83 /I (1) | 170 | I170/V (5) |
86 | P86/Q (11) | 178 | T178/V (2) |
87 | A87/S (2) | ||
94 | M94/L (10) | ||
107 | V107/I (1) |
Position NS3a
| Subtype 2cb
|
---|---|
15 | D15G/S (9) |
33 | V33I (3) |
35 | V35I (1) |
134 | S134T (4) |
146 | P146S (1) |
177 | V177I (1) |
aPositions |
bSubtype 4 |
aPositions |
bSubtype 4 |
---|---|---|---|
12 | G12/R (1) | 98 | T98/A (1) |
13 | L13/M (8) | 102 | A102/S (8) |
14 | F14/L (8) | 105 | Y105/F (7) |
15 | S15/G (7) | 114 | I114/V (8) |
18 | V18/I (8) | 122 | T122/N (1) |
24 | R24/K (1) | 127 | L127/I (5) |
33 | V33/I (4) | 133 | S133/A (1) |
47 | A47/S (3) | 134 | I134/T (8) |
48 | V48/I (7) | 147 | M147/L (8) |
61 | A61/S (8) | 150 | R150/A (8) |
92 | R92/K (8) | 172 | V172/I (1) |
95 | A95/T (1) | 179 | M179/A (1) |