Background
Methods
Subjects
Gensini score
Human genomic DNA extraction
MIF − 794 CATT5–8 genotyping
Analysis of serum MIF levels
Statistic analyses
Results
Frequencies of MIF − 794 alleles and genotypes of CAD patients and controls
Control group (n = 186) | CHD group (n = 70) | F/×2
|
P value | |
---|---|---|---|---|
Mean age(year)a
| 60.78 ± 9.26 | 66.71 + 10.25 | 1.24 | 0.081 |
Ganderb
(male/female) | 84/102 | 44/26 | 6.37 | 0.012 |
cigarette smoking(%)b
| 31.6% | 33.3% | 0.236 | 0.627 |
Drinking(%)b
| 18.3% | 24.3% | 0.677 | 0.41 |
Hypertension(%)b,c
| 66.7% | 68.6% | 0.054 | 0.817 |
Hyperlipidemia(%)b,d
| 16.7% | 37.1% | 6.76 | 0.009 |
Diabetes(%)b,e
| 3.3% | 7.1% | 0.92 | 0.337 |
Polymorphisms | Genotypes/alleles | CHD group n = 70 | Control group n = 186 | x2
|
P valuea
|
---|---|---|---|---|---|
-794CATT | 5/5 | 11(15.7) | 31(17.6) | ||
6/6 | 16(22.9) | 30(17.0) | |||
7/7 | 3(4.3) | 7(4.0) | |||
5/6 | 22(31.4) | 56(31.8) | |||
5/7 | 10(14.3) | 24(13.6) | |||
6/7 | 8(11.4) | 26(14.8) | |||
6/8 | 0(0) | 1(0.54) | 0.464 | 0.834 | |
5 | 54(38.6) | 132(35.5) | |||
6 | 62(44.3) | 190(51.1) | |||
7 | 24(17.1) | 49(13.2) | |||
8 | 0(0) | 1(0.27) | 0.003 | 0.959 |
The plasma concentration of MIF
CHD group (n = 70) | Control group (n = 186) | P* value | ||
mean concentration of MIF + SD | 65.75 ± 6.32 | 51.13 ± 7.33 | ||
median of MIF | 65.66 | 50.01 | 0.00 | |
alleles 5 (2n = 186) | alleles 6 (2n = 252) | alleles 7 (2n = 73) | P# value | |
mean concentration of MIF + SD | 58.68 + 7.80 | 60.22 + 9.77 | 62.09 + 5.32 | |
median of MIF | 58.275 + 1.42 | 59.75 + 0.98 | 61.79 + 0.51 | 0.373 |
Frequencies of MIF− CATT5–8 alleles among CAD patients
CHD class (Gensini’s intergral degree) | the allele frequency of MIF-794 CATT5–8
| |
---|---|---|
5 | 6 | |
≦20 | 16 | 10 |
20–40 | 14 | 10 |
≧40 | 13 | 4 |
x2
| 1.55 | |
Pa value | 0.46 |