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Journal of Mammary Gland Biology and Neoplasia
Erschienen in: Journal of Mammary Gland Biology and Neoplasia 4/2020

18.11.2020 | Review Paper

Mammary Organoids and 3D Cell Cultures: Old Dogs with New Tricks

verfasst von: Jakub Sumbal, Zuzana Budkova, Gunnhildur Ásta Traustadóttir, Zuzana Koledova

Erschienen in: Journal of Mammary Gland Biology and Neoplasia | Ausgabe 4/2020

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Abstract

3D cell culture methods have been an integral part of and an essential tool for mammary gland and breast cancer research for half a century. In fact, mammary gland researchers, who discovered and deciphered the instructive role of extracellular matrix (ECM) in mammary epithelial cell functional differentiation and morphogenesis, were the pioneers of the 3D cell culture techniques, including organoid cultures. The last decade has brought a tremendous increase in the 3D cell culture techniques, including modifications and innovations of the existing techniques, novel biomaterials and matrices, new technological approaches, and increase in 3D culture complexity, accompanied by several redefinitions of the terms “3D cell culture” and “organoid”. In this review, we provide an overview of the 3D cell culture and organoid techniques used in mammary gland biology and breast cancer research. We discuss their advantages, shortcomings and current challenges, highlight the recent progress in reconstructing the complex mammary gland microenvironment in vitro and ex vivo, and identify the missing 3D cell cultures, urgently needed to aid our understanding of mammary gland development, function, physiology, and disease, including breast cancer.
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Metadaten
Titel
Mammary Organoids and 3D Cell Cultures: Old Dogs with New Tricks
verfasst von
Jakub Sumbal
Zuzana Budkova
Gunnhildur Ásta Traustadóttir
Zuzana Koledova
Publikationsdatum
18.11.2020
Verlag
Springer US
Erschienen in
Journal of Mammary Gland Biology and Neoplasia / Ausgabe 4/2020
Print ISSN: 1083-3021
Elektronische ISSN: 1573-7039
DOI
https://doi.org/10.1007/s10911-020-09468-x

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