Background
Hepatocellular carcinoma (HCC) is the third leading cause of cancer mortality worldwide with poor survival and unsatisfied prognosis [
1‐
3]. Over decades, the incidence of HCC has been dramatically increased especially in hepatitis B or C virus (HBV or HCV) infection induced phenotype [
4]. Until now, surgical hepatic resection and liver transplantation are still the main curative treatment for HCC patients. Although great advances have been made in treatment and diagnosis, the prognosis of HCC remains limited, with its survival rates under 20% at 5 years [
5]. Therefore, it’s an urgent need to understand the molecular mechanisms responsible for the pathogenesis of HCC and to identify effective treatment strategies. However, tumor recurrence rates remain a major concern for the exhibition of active hepatitis or cirrhosis in surrounding non-tumor liver tissues, even in patients who have received curative treatments [
6,
7]. A better understanding of the molecular mechanisms that can distinguish progressive from non-progressive HCC is indispensable for exploring novel prognostic markers and therapeutic targets which may guide the surveillance after liver transplantation.
Recent evidences support that microRNAs (miRNAs) serve as potential indicators for diagnosis and prognosis of cancers [
8]. miRNAs are small noncoding RNAs, which contain 20 ~ 23 nucleotides, processed from pri-miRNAs and contribute to post-transcriptional regulation of target gene expression through binding directly to the specific sequences of target genes’ 3′-UTRs [
8]. Researches show that miRNAs have effects on cell proliferation, migration, and apoptosis via making a difference in the stability or translation of target mRNA. Additionally, miRNAs are considered as crucial participators in tumor progression through influencing multiple biological functions and pathways [
9]. Previous study has demonstrated that miR-214-3p is correlated with tumor onset and progression [
10]. It has been reported that miR-214-3p is downregulated in HCC tissues and closely related to fibrotic stages [
11,
12]; however, the biological functions of miR-214-3p in HCC and its position in HCC prognosis after transplantation remain unclear.
In our current study, the expression of miR-214-3p in the formalin-fixed paraffin-embedded (FFPE) tumor tissues from HCC patients was detected, and the correlation of miR-214-3p expression with lymph node metastasis, recurrence, pathological T stage, and age was analyzed as well. Moreover, a further insight into the function of miR-214-3p in regulating HCC cell proliferation, cell cycle, and apoptosis was gained by overexpressing miR-214-3p in human HCC cells.
Methods
Patients and tissue samples
A cohort of 98 patients undergoing liver transplantation for HCC was obtained with their follow-up data from the Nanfang Hospital of Southern Medical University from January 2006 to November 2011. All the patients were followed until December 2011. The median recurrence-free period was 12 months for patients with HCC recurrence compared to 65 months for patients without HCC recurrence. All of these 98 patients enrolled in this study met the transplantation criteria for HCC [
13]. HCC samples were from the paraffin embedded archival tissue blocks and the normal liver tissues were from the liver hemangioma resection. The clinicopathological parameters of patients with HCC were summarized in Table
1. Informed consents from all patients were provided according to the protocols approved by the Institutional Review Boards of the Nanfang Hospital of Southern Medical University.
Table 1
Clinicopathology parameters in 98 HCC patients according to high- or low miR-214-3p expression level
Age | 98 | 56.60 ± 6.14 | 54.06 ± 8.73 | 0.327*
|
Gender |
Male | 83 | 44 | 39 | 0.742#
|
Female | 15 | 6 | 9 | |
Liver disease |
HBV | 93 | 46 | 47 | 1.000∆
|
Others | 5 | 3 | 2 | |
Cirrhosis |
Positive | 94 | 46 | 48 | 1.000∆
|
Negative | 4 | 2 | 2 | |
Tumor stage |
I + II | 65 | 32 | 33 | 0.791#
|
III | 33 | 18 | 15 | |
Histologic grade |
Differentiated | 87 | 41 | 46 | 0.068∆
|
Undifferentiated | 11 | 8 | 3 | |
Milan criteria |
In | 53 | 23 | 30 | 0.163#
|
Out | 45 | 26 | 19 | |
Tumor size (cm) |
≤ 4.5 | 59 | 24 | 35 | 0.016#
|
> 4.5 | 39 | 25 | 14 | |
Multinodular |
Positive | 43 | 25 | 18 | 0.044#
|
Negative | 55 | 24 | 31 | |
Vascular invasion |
Positive | 22 | 16 | 6 | 0.025#
|
Negative | 77 | 33 | 43 | |
Serum AFP (ng/ml) |
≤ 400 | 62 | 29 | 33 | 1.000#
|
> 400 | 36 | 19 | 17 | |
Overall survival | 41/98 | 11/49 | 30/49 | |
HCC recurrence | 57/98 | 37/49 | 20/49 | |
Cell culture and transfection
HepG2, HUH-7, SNU398 and L-O2 cell lines used in this study were purchased from the ATCC (Manassas, VA). All cells then were cultured in EMEM and supplemented with 10% (v/v) heat-inactivated fetal bovine serum (FBS, Gemini Bio-Products, Sacramento, CA) and antibiotics (98 U/ml penicillin and 98 μg/ml streptomycin) at 37 °C in a humidified atmosphere of 5% CO2. Double-stranded RNAs that mimic endogenous precursor miR-214-3p (Invitrogen-Life Technologies, Carlsbad, CA) as well as negative oligonucleotide control was transfected into cells using Oligofectamine (Thermo Scientific, Waltham, MA) according to the manufacturer’s instruction.
RNA isolation and Taqman real-time PCR
Total RNA was isolated and then reverse transcribed to cDNA with the stem-loop RT primer for miR-214-3p. miR-214-3p expression was normalized and quantificated using U6 small RNA as an internal control. For miR-214-3p analysis, primers were 5′-GCATCCTGCCTCCACATGCAT-3′ and 5′-GCGCTGAGGAATAATAG AGTATGTAT-3′. PCR primers for the internal control U6 were 5′-TGACTTCCAAG TACCATCGCCA-3′ and 5′-TTGTAGAGGTAGGTGTGCAGCAT-3′. The relative expression levels were calculated using the 2−ΔΔCt method. All the experiments were run in triplicate.
Cell proliferation assay
Cell proliferation assay was carried out using cell Titer 96 Aqueous one Solution Cell Proliferation Assay (Promega, Madison, WI) follow in the manufacturer’s protocol. Three independent experiments were done.
Cell cycle analysis
HepG2 and HUH-7 cells were collected in the log phase of growth and incubated for 24 h. Then the cells were trypsinized, washed with PBS twice, and fixed overnight in cold 75% ethanol at 4 °C. After that, the fixed cells were stained with propidium iodide, follow by examination using flow cytometer (BD Biosciences, San Jose, CA). Finally, DNA histograms were analyzed with modified software. Three independent experiments were done.
Apoptosis analysis
A total of 5 × 105 cells were harvested and centrifuged for apoptotic evaluation. Propidium iodide (BD Bioscience) and the fluorescein isothiocyanate-conjugated (FITC) anti-human Annexin V Apoptosis Detection Kit I (BD Pharmingen) was used to characterize cells according to the manufacturer’s instructions. Labeled cells were detected using the fluorescence activated cell sorting (FACS) Aria II Cell Sorter System (BD Biosciences), followed by data analysis using the Diva program (BD Biosciences). Three independent experiments were done.
Luciferase activity assay
For luciferase reporter assay, HEK293T cells were cultured in 48-well plates and then cotransfected with 10 ng pGL3 cm-MELK-3′ UTR-Wt or pGL3 cm-MELK-3′ UTR-Mut, 30 pmol of miR-214-3p precursor or NC oligonucleotides, and 2 ng of pRL-TK (Ruibo, Guangzhou, China). After transfection for 72 h, cells were collected separately and then analyzed following the Dual-Luciferase Reporter Assay protocol (Promega, Madison, WI). The data were presented as relative luciferase activity. Three independent experiments were done.
Immunohistochemical (IHC) staining
Briefly, before antigen retrieval in citrate buffer, tissue sections were dewaxed and subsequently rehydrated in graded series of ethanols. After that, the sections were incubated overnight with antibody against MELK (Epitomics, Burlingame, USA; 1:200) at 4 °C, followed by incubation with an HRP-conjugated secondary antibody and DAB (Dako, Carpenteria, CA). DAB was used for color development, compared with dark brown staining was considered positive.
Western blot analysis
Cells were harvested and lysed in lysis buffer supplemented with proteinase inhibitor cocktail on ice for 20 min. Cell lysates were resolved by SDS-PAGE and transferred to PVDF membranes (Millipore). The membranes were blocked for 1 h in 5% non-fat dry milk and incubated with primary MELK (Epitomics, Burlingame, USA or GAPDH (Cell signaling technology, Danvers, USA) antibodies at 4 °C overnight. Then the membranes were incubated with HRP-conjugated secondary antibodies and detected with ECL Plus (Millipore).
Statistical analysis
The SPSS version 17.0 (SPSS Inc. Chicago, IL) was used for statistical analysis in this study. Comparisons between two groups were performed using Student’s t test. Correlations between clinicopathologic characteristics and immunohistochemical variables were analyzed using Chi square test or Fisher’s exact test. The Kaplan–Meier method was selected to graph survival curves. Log-rank statistic was applied to calculate the differences between the groups. The impact of prognostic factors on RFS and OS were analyzed by Cox proportional hazard models. A two-sided P value less than 0.05 was considered statistically significant.
Discussion
In recent decades, miRNAs have been considered as contributive regulators involved in transcriptional regulation, cell differentiation, tumorigenesis, and other biological processes [
14]. Globally aberrant miRNA expression profiles of tumors have provided valuable insights into the molecular pathways of oncogenesis [
15]. Nowadays, more than 2000 human miRNAs have been reported as regulational factors in cell proliferation, migration, and invasion of tumors [
16]. Newly papers indicated that upregulation of miR-214-3p was found in breast cancer patients with osteolytic bone metastasis, and a knock-in miR-214-3p remarkably increased bone resorption by straightly targeting Traf3 to promote osteoclast activity and bone-resorbing activity [
17,
18]. However, other studies demonstrated that miR-214-3p was significantly downregulated in two esophageal squamous cancer cell lines compared with esophageal epithelial cells [
19]. Further investigations pointed out that downregulation of miR-214-3p suppressed chemoresistance in esophageal cancer cells by targeting both survivin and CUG-BP1 [
20]. The anti-apoptotic Bcl-2 family member MELK/A1 is one of these PRDI-BF1/Blimp-1 target genes. MELK as a target of miR-214-3p provides a novel perspective on the mechanisms underlying HCC proliferation and resistance to apoptosis [
21]. Abundant MELK expression was detected in the bone marrow as well as in some other tissues [
22,
23]. Specifically, a connection was found in clinical samples between the MELK expression and the progression of stomach carcinoma [
24].
Our studies showed that miR-214-3p expression was decreased in both HCC tissues and HCC cell lines, which were consistent with previous results [
11]. It has been reported that miR-214-3p expression is strongly related to with fibrotic stages [
12]. Interestingly, in this study, we found that miR-214-3p expression is also closely associated with recurrence and living status of liver transplant patients. Moreover, downregulation of miR-214-3p was associated with poor survival and tumor recurrence in HCC patients. Moreover, miR-214-3p restoration inhibited cell cycle progression and accelerated apoptosis in vitro. To our best knowledge, this is the first study showing that miR-214-3p regulates cellular proliferation in HCC cells and links to the prognosis of HCC. In all, this study shown that miR-214-3p was decreased in HCC tissues and the expression level of miR-214-3p might be a significant prognostic marker for HCC patients. Based on gain-of-function approach, it is suggested that miR-214-3p could remarkably block HCC cell proliferation and induce apoptosis in vitro by directly targeting MELK 3′-UTR.
Conclusions
Collectively, our data not only demonstrates novel insights regarding miR-214-3p function and the potential mechanisms of HCC cell proliferation, but also indicates a possible regulation pathway for MELK and a potential therapeutic strategy for HCC treatment.
Authors’ contributions
LY carried out the experiments, and also revised the manuscript. LY and CY carried out the experiments and drafted the manuscript. XQ, DNN, DH and GYJ carried out the experiments. LCH participated in the design of the study and performed the statistical analysis. WSH conceived of the study, and participated in its design and coordination and helped to draft the manuscript. All authors read and approved the final manuscript.
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