Background
Methods
Participants
Placebo (n = 10) | 9 g MPC (n = 10) | |
---|---|---|
Age (years) | 45.5 ± 5.8 | 48.6 ± 4.6 |
Height (cm) | 175.9 ± 5.6 | 179.5 ± 6.1 |
Weight (kg) | 79.3 ± 9.1 | 76.3 ± 8.4 |
BMI (kg/m2) | 24.6 ± 2.4 | 24.4 ± 2.8 |
% Body fat | 19.3 ± 7.6 | 18.2 ± 5.7 |
HDL cholesterol (mmol/L) | 1.36 ± 0.30 | 1.52 ± 0.59 |
LDL cholesterol (mmol/L) | 4.13 ± 1.10 | 3.37 ± 0.98 |
Triglycerides (mmol/L) | 1.27 ± 0.52 | 1.24 ± 0.55 |
HOMA-IR | 1.81 ± 1.33 | 1.18 ± 0.34 |
Leg press 1RM (kg) | 89.9 ± 20.3 | 92.5 ± 23.8 |
Leg extension 1RM (kg) | 46.9 ± 11.2 | 45.3 ± 15.7 |
Study beverages
Functional milk product | Carbohydrate placebo | |
---|---|---|
Energy (kJ) | 504 | 491 |
Carbohydrate (g) | 13.8 | 28.0 |
Fat (g) | 3.2 | 0.6 |
Protein (g) | 9.2 | 0.1 |
Essential amino acids (g) | 4.0 | - |
Threonine (g) | 0.43 | - |
Valine (g) | 0.53 | - |
Isoleucine (g) | 0.46 | - |
Leucine (g) | 0.87 | - |
Phenylalanine (g) | 0.42 | - |
Lysine(g) | 0.72 | - |
Histidine (g) | 0.23 | - |
Methionine (g) | 0.21 | - |
Experimental design
Plasma measurements
Immunoblotting
Real-time polymerase chain reaction
LAT1 (Forward) | CATCCTCCAGGCTCTTCTTCGTG |
LAT1 (Reverse) | AAGGCGTAGAGCAGCGTCAT |
SNAT2 (Forward) | GCAGTGGAATCCTTGGGCTT |
SNAT2 (Reverse) | AAAGACCCTCCTTCATTGGCAG |
CAT1 (Forward) | TCTCATTTAAGGTTCCCTTCCTG |
CAT1 (Reverse) | ACAGGCCATAGCCAAAGTAGA |
PAT1 (Forward) | CTTCTGCCGCAGGCTGAATAAA |
PAT1 (Reverse) | CAGGAAGAAGTCCACAACACG |
SESTRIN1 (Forward) | TTTCGTGTCCAGGACTATTGC |
SESTRIN1 (Reverse) | ACTGTCCCACATCTGGATAAAGG |
SESTRIN2 (Forward) | CAACCTCTTCTGGAGGCACTT |
SESTRIN2 (Reverse) | CCTGCTCAGGAGTCAGGTCA |
SESTRIN3 (Forward) | CAGGCAGCAACTTTGGGATTGT |
SESTRIN3 (Reverse) | AGACGCCTCTTCATCTTCCCTT |
Gene | Sequence |
---|---|
EMC7 (Forward) | GGGCTGGACAGACTTTCTAATG |
EMC7 (Reverse) | CTCCATTTCCCGTCTCATGTCAG |
VCP (Forward) | AAACTCATGGCGAGGTGGAG |
VCP (Reverse) | TGTCAAAGCGACCAAATCGC |
CHMP2A (Forward) | CGCTATGTGCGCAAGTTTGT |
CHMP2A (Reverse) | GGGGCAACTTCAGCTGTCTG |
C1orf43 (Forward) | CTATGGGACAGGGGTCTTTGG |
C1orf43 (Reverse) | TTTGGCTGCTGACTGGTGAT |
HPRT (Forward) | CCTGGCGTCGTGATTAGTGAT |
HPRT (Reverse) | TCGAGCAAGACGTTCAGTCC |