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01.12.2017 | Research article | Ausgabe 1/2017 Open Access

Journal of Orthopaedic Surgery and Research 1/2017

MiRNA expression in the cartilage of patients with osteoarthritis

Zeitschrift:
Journal of Orthopaedic Surgery and Research > Ausgabe 1/2017
Autoren:
Marta Kopańska, Dariusz Szala, Joanna Czech, Natalia Gabło, Krzysztof Gargasz, Mateusz Trzeciak, Izabela Zawlik, Sławomir Snela
Wichtige Hinweise
An erratum to this article is available at http://​dx.​doi.​org/​10.​1186/​s13018-017-0577-0.

Abstract

Background

Osteoarthritis (OA), the most prevalent disease of articulating joints, is a complex multifactorial disease caused by genetic, mechanical, and environmental factors. In this research, we evaluated miRNA expression in OA.

Methods

Forty tissue samples from 29 patients undergoing joint replacement for OA were evaluated. Tissue from two control patients undergoing hip replacement not related to OA was used as a control. Total RNA (containing miRNA species) from cartilage was isolated using a mirVana miRNA Isolation Kit. Expression of 19 miRNAs was assessed by real-time quantitative polymerase chain reaction.

Results

Expression of four miRNAs, miR-138-5p, miR-146a-5p, miR-335-5p, and miR-9-5p, was significantly upregulated in OA tissues (patients vs. control group).

Conclusions

These findings may contribute to disease prevention and the development of therapeutic targets for OA.
Literatur
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