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01.12.2017 | Research | Ausgabe 1/2017 Open Access

Virology Journal 1/2017

Molecular evolution of two asymptomatic echovirus 6 strains that constitute a novel branch of recently epidemic echovirus 6 in China

Zeitschrift:
Virology Journal > Ausgabe 1/2017
Autoren:
Hao Sun, Xiaoqin Huang, Keqin Lin, Kai Huang, Jiayou Chu, Zhaoqing Yang, Shaohui Ma
Wichtige Hinweise

Electronic supplementary material

The online version of this article (doi:10.​1186/​s12985-017-0809-2) contains supplementary material, which is available to authorized users.

Abstract

Background

Echovirus 6 (E6) infections are associated with aseptic meningitis and acute flaccid paralysis (AFP). But some infections, sometimes most of them, are asymptomatic. The mechanism of E6 virulence is unknown. Analyses of the molecular evolution of asymptomatic E6 may help understand why the infections show different manifestations.

Methods

Ninety-six stool samples of healthy children in Yunnan, China were collected and two E6 strains were isolated from them. The whole genomes of these two E6 strains were sequenced, and their molecular evolution was analyzed.

Results

The results showed that the two E6 strains may be derived from KJ7724XX strains, which were predominant in AFP patients in Shangdong in 2011. The evolution was accelerated when the two E6 strains formed, although no positive selection site was found. The 11 exclusive mutations on which selection force significantly changed were found in the 2C, 3AB and 3C genes.

Conclusion

There are some E6 strains which did not cause the disease in the children of Yunnan. These E6 strains maybe come from a recombinant E6 strain which was associated with the outbreak of AFP in Shangdong in 2011. However, some new mutations were found in the 2C, 3AB and 3C genes of these asymptomatic strains, and these mutations may be constraint by the natural selection and could be potentially responsible for clinical presentations.
Zusatzmaterial
Additional file 1: Figure S1. Polygenetic tree based on the VP1 sequences of EVs. The tree includes nearly all of the E6 strains found in China by 2016. The black circles indicate the strains that we found, and the black triangles indicate the closest strains to them. Figure S2. Similarity plot and bootscanning analysis queried by K843. (a) The similarity plot between K843 and other EVs. (b) The bootscanning analysis for K843. The analyses were conducted via Simplot v3.5.1 using a sliding window of 400 nucleotides moving in steps of 20 nucleotides. (PDF 919 kb)
12985_2017_809_MOESM1_ESM.pdf
Literatur
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