nach oben

Journal of Endocrinological Investigation

Erschienen in:

01.07.2018 | Original Article

Molecular study of human sperm RNA: Ropporin and CABYR in asthenozoospermia

verfasst von: M. Pelloni, D. Paoli, M. Majoli, F. Pallotti, T. Carlini, A. Lenzi, F. Lombardo

Erschienen in: Journal of Endocrinological Investigation | Ausgabe 7/2018

Einloggen, um Zugang zu erhalten

Abstract

Background

Sperm motility is an essential aspect of human fertility. Sperm contain an abundance of transcripts, thought to be remnants of mRNA, which comprise a genetic fingerprint and can be considered a historic record of gene expression during spermatogenesis. The aberrant expression of numerous genes has been found to contribute to impaired sperm motility; these include ROPN1 (rhophilin associated tail protein 1), which encodes a component of the fibrous sheath of the mammalian sperm flagella, and CABYR (calcium-binding tyrosine-(Y)-phosphorylation-regulated protein), which plays an important role in calcium activation and modulation. The aim of this study was to investigate ROPN1 and CABYR gene co-expression in asthenozoospermic semen samples in comparison with normozoospermic samples.

Methods

We studied 120 semen samples (60 normozoospermic and 60 asthenozoospermic) from Caucasian patients attending our centre for an andrological check-up. Total RNA was extracted from purified spermatozoa with RNeasy mini kit. ROPN1 and CABYR mRNA expression was analysed using RT-qPCR. Continuous variables were described as means ± standard deviations.

Results

ROPN1 and CABYR mRNA were simultaneously downregulated in asthenozoospermic in comparison with normozoospermic samples. There was also a positive correlation between total progressive motility and ROPN1 and CABYR gene expression and between total motile sperm number and ROPN1 and CABYR gene expression.

Conclusions

The results demonstrated downregulation of both ROPN1 and CABYR in asthenozoospermic samples and importantly, a positive correlation between the expression of the two genes, suggesting that ROPN1 and CABYR co-expression is a prerequisite for normal flagellar function and sperm motility.

O’Flynn O’Brien KL, Varghese AC, Agarwal A (2010) The genetic causes of male factor infertility: a review. Fertil Steril 93:1–12CrossRefPubMed

Chen J, Wang Y, Wei B, Lai Y, Yan Q, Gui Y, Cai Z (2011) Functional expression of ropporin in human testis and ejaculated spermatozoa. J Androl 32:26–32CrossRefPubMed

Martins RP, Krawetz SA (2005) RNA in human sperm. Asian J Androl 7:115–120CrossRefPubMed

Jodar M, Selvaraju S, Sendler E, Diamond MP, Krawetz SA, Reproductive Medicine Network (2013) The presence, role and clinical use of spermatozoal RNAs. Hum Reprod Update 19:604–624 (Review) CrossRefPubMedPubMedCentral

Paoli D, Pelloni M, Gallo M, Coltrinari G, Lombardo F et al (2017) Sperm glyceraldehyde 3-phosphate dehydrogenase gene expression in asthenozoospermic spermatozoa. Asian J Androl 19:409–413CrossRefPubMed

Ostermeier GC, Miller D, Huntriss JD, Diamond MP, Krawetz SA (2004) Reproductive biology: delivering spermatozoan RNA to the oocyte. Nature 429:154CrossRefPubMed

Lalancette C, Miller D, Li Y, Krawetz S (2008) Paternal contributions: new functional insights for spermatozoal RNA. J Cell Biochem 104:1570–1579CrossRefPubMedPubMedCentral

Dadoune JP (2009) Spermatozoal RNAs: what about their functions? Microsc Res Tech 72:536–551CrossRefPubMed

Bansal SK, Gupta N, Sankhwar SN, Rajender S (2015) Differential genes expression between fertile and infertile spermatozoa revealed by transcriptome analysis. PLoS One 10:e0127007CrossRefPubMedPubMedCentral

10.

Jodar M, Sendler E, Moskovtsev SI, Librach CL, Goodrich R et al (2015) Absence of sperm RNA elements correlates with idiopathic male infertility. Sci Transl Med 7:295re6CrossRefPubMedPubMedCentral

11.

Wang H, Zhou Z, Xu M, Li J, Xiao J et al (2004) A spermatogenesis-related gene expression profile in human spermatozoa and its potential clinical applications. J Mol Med (Berl) 82:317–324CrossRef

12.

Shen S, Wang J, Liang J, Zhu C (2015) Low-expressed testis-specific calcium-binding protein CBP86-IV (CABYR) is observed in idiopathic asthenozoospermia. World J Urol 33:633–638CrossRefPubMed

13.

Fujita A, Nakamura K, Kato T, Watanabe N, Ishizaki T et al (2000) Ropporin, a sperm-specific binding protein of rhophilin that is localized in the fibrous sheath of sperm flagella. J Cell Sci 113:103–112PubMed

14.

Richardson RT, Yamasaki N, O’Rand MG (1994) Sequence of a rabbit sperm zona pellucida binding protein and localization during the acrosome reaction. Dev Biol 165:688–701CrossRefPubMed

15.

Fiedler SE, Sisson JH, Wyatt TA, Pavlik JA, Gambling TM et al (2012) Loss of ASP but not ROPN1 reduces mammalian ciliary motility. Cytoskeleton (Hoboken) 69:22–32CrossRef

16.

Nakamura K, Fujita A, Murata T, Watanabe G, Mori C et al (1999) Rhophilin, a small GTPase Rho-binding protein, is abundantly expressed in the mouse testis and localized in the principal piece of the sperm tail. FEBS Lett 445:9–13CrossRefPubMed

17.

Eddy EM, Toshimori K, O’Brien DA (2003) Fibrous sheath of mammalian spermatozoa. Microsc Res Tech 61:103–115CrossRefPubMed

18.

Carr DW, Fujita A, Stentz CL, Liberty GA, Olsen GE et al (2001) Identification of sperm specific proteins that interact with A-kinase anchoring proteins in a manner similar to the type II regulatory subunit of PKA. J Biol Chem 276:17332–17338CrossRefPubMed

19.

Fiedler SE, Bajpai M, Carr DW (2007) Identification and characterization of RHOA-interacting proteins in bovine spermatozoa. Biol Reprod 78:184–192CrossRefPubMed

20.

Lea IA, Widgren EE, O’Rand MG (2004) Association of sperm protein 17 with A-kinase anchoring protein 3 in flagella. Reprod Biol Endocrinol 2:57CrossRefPubMedPubMedCentral

21.

Hanlon Newell AE, Fiedler SE, Ruan JM, Pan J, Wang PJ et al (2008) Protein kinase A RII-like (R2D2) proteins exhibit differential localization and AKAP interaction. Cell Motil Cytoskelet 65:539–552CrossRef

22.

Li YF, He W, Mandal A, Kim YH, Digilio L et al (2011) CABYR binds to AKAP3 and Ropporin in the human sperm fibrous sheath. Asian J Androl 13:266–274CrossRefPubMedPubMedCentral

23.

Naaby-Hansen S, Mandal A, Wolkowicz MJ, Sen B, Westbrook VA et al (2002) CABYR, a novel calcium-binding tyrosine phosphorylation-regulated fibrous sheath protein involved in capacitation. Dev Biol 242:236–254CrossRefPubMed

24.

Naaby-Hansen S (2012) Functional and immunological analysis of the human sperm proteome. Dan Med J 59:4414

25.

Hong CY, Chiang BN, Turner P (1984) Calcium ion is the key regulator of human sperm function. Lancet 2:1449–1451CrossRefPubMed

26.

Kim YH, Jha KN, Mandal A, Vanage G, Farris E et al (2005) Translation and assembly of CABYR coding region B in fibrous sheath and restriction of calcium binding to coding region. A Dev Biol 286:46–56CrossRefPubMed

27.

Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T) method. Methods 25:402–408CrossRefPubMed

28.

Curi SM, Ariagno JI, Chenlo PH, Mendeluk GR, Pugliese MN et al (2003) Asthenozoospermia: analysis of a large population. Arch Androl 49:343–349CrossRefPubMed

29.

Bozhedomov VA, Nikolaeva MA, Ushakova IV, Lipatova NA, Bozhedomova GE et al (2015) Functional deficit of sperm and fertility impairment in men with antisperm antibodies. J Reprod Immunol 112:95–101CrossRefPubMed

30.

Pereira R, Sá R, Barros A, Sousa M (2017) Major regulatory mechanisms involved in sperm motility. Asian J Androl 19:5–14PubMed

31.

Linck RW, Chemes H, Albertini DF (2016) The axoneme: the propulsive engine of spermatozoa and cilia and associated ciliopathies leading to infertility. J Assist Reprod Genet 33:141–156 (Review) CrossRefPubMedPubMedCentral

32.

Amer MK, Mostafa RM, Fathy A, Saad HM, Mostafa T (2015) Ropporin gene expression in infertile asthenozoospermic men with varicocele before and after repair. Urology 85:805–808CrossRefPubMed

33.

Yanagimachi R (1994) Fertility of mammalian spermatozoa: its development and relativity. Zygote 2:371–372 (Review) CrossRefPubMed

34.

Li YF, He W, Kim YH, Mandal A, Digilio L et al (2010) CABYR isoforms expressed in late steps of spermiogenesis bind with AKAPs and ropporin in mouse sperm fibrous sheath. Reprod Biol Endocrinol 8:101CrossRefPubMedPubMedCentral

35.

Young SA, Miyata H, Satouh Y, Aitken RJ, Baker MA et al (2016) CABYR is essential for fibrous sheath integrity and progressive motility in mouse spermatozoa. J Cell Sci 129:4379–4387CrossRefPubMed

Titel: Molecular study of human sperm RNA: Ropporin and CABYR in asthenozoospermia
verfasst von: M. Pelloni
D. Paoli
M. Majoli
F. Pallotti
T. Carlini
A. Lenzi
F. Lombardo
Publikationsdatum: 01.07.2018
Verlag: Springer International Publishing
Erschienen in: Journal of Endocrinological Investigation / Ausgabe 7/2018
Elektronische ISSN: 1720-8386
DOI: https://doi.org/10.1007/s40618-017-0804-x

Leitlinien kompakt für die Innere Medizin

Mit medbee Pocketcards sicher entscheiden.

^{Seit 2022 gehört die medbee GmbH zum Springer Medizin Verlag}

Kostenlos registrieren

Neu im Fachgebiet Innere Medizin

Echinokokkose medikamentös behandeln oder operieren?

06.05.2024 DCK 2024 Kongressbericht

Die Therapie von Echinokokkosen sollte immer in spezialisierten Zentren erfolgen. Eine symptomlose Echinokokkose kann – egal ob von Hunde- oder Fuchsbandwurm ausgelöst – konservativ erfolgen. Wenn eine Op. nötig ist, kann es sinnvoll sein, vorher Zysten zu leeren und zu desinfizieren.

Umsetzung der POMGAT-Leitlinie läuft

03.05.2024 DCK 2024 Kongressbericht

Seit November 2023 gibt es evidenzbasierte Empfehlungen zum perioperativen Management bei gastrointestinalen Tumoren (POMGAT) auf S3-Niveau. Vieles wird schon entsprechend der Empfehlungen durchgeführt. Wo es im Alltag noch hapert, zeigt eine Umfrage in einem Klinikverbund.

Proximale Humerusfraktur: Auch 100-Jährige operieren?

01.05.2024 DCK 2024 Kongressbericht

Mit dem demographischen Wandel versorgt auch die Chirurgie immer mehr betagte Menschen. Von Entwicklungen wie Fast-Track können auch ältere Menschen profitieren und bei proximaler Humerusfraktur können selbst manche 100-Jährige noch sicher operiert werden.

Die „Zehn Gebote“ des Endokarditis-Managements

30.04.2024 Endokarditis Leitlinie kompakt

Worauf kommt es beim Management von Personen mit infektiöser Endokarditis an? Eine Kardiologin und ein Kardiologe fassen die zehn wichtigsten Punkte der neuen ESC-Leitlinie zusammen.

Update Innere Medizin

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert.

Newsletter bestellen

Springer Medizin

Abstract

Background

Methods

Results

Conclusions

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 7/2018

“Madonna of the carnation”: Leonardo da Vinci (1452–1519)

One-hour post-load plasma glucose level is associated with a worse metabolic profile in children with GH deficiency

A case of drug-induced Graves’ Orbitopathy after combination therapy with Tremelimumab and Durvalumab

Long-term response to recombinant human growth hormone treatment: a new predictive mathematical method

Impaired taste sensation in type 2 diabetic patients without chronic complications: a case–control study