Mutations in NFKB2and potential genetic heterogeneity in patients with DAVID syndrome, having variable endocrine and immune deficiencies

Deficit in anterior pituitary function (hypopituitarism) and common variable immune deficiency (CVID, MIM [607594]) represent two very different clinical presentations. Mutations in several genes have been associated with each of these conditions, but until recently no gene has been associated with deficiencies in both systems in the same patients. Several years ago we identified such patients in three families as part of a national screen for hypopituitarism in France, and defined a novel disorder which we named DAVID syndrome (for Deficient Anterior pituitary with Variable Immune Deficiency) [1]. At the time it was not obvious whether these patients represented a shared molecular etiology or a coincidental overlap of two otherwise uncommon pediatric conditions, although there were at least two previous isolated case reports with similar clinical descriptions [2],[3]. Chen et al recently reported mutations in the NFKB2 gene in two families from Utah with features consistent with our definition of DAVID syndrome (immunodeficiency with hypogammaglobulinemia, plus central pituitary deficiency), one mutation of which was consistent with de novo germ line origin [4]. Several additional groups have since reported mutations near the C-terminus of NFKB2, in patients with features overlapping DAVID syndrome, although not all patients showed the endocrine aspect of the phenotype [5]-[7].

Research ethics approval was obtained from the review boards of the Centre de Recherche du CHU Ste-Justine, and of the Assistance Publique-Hôpitaux de Marseille. Written informed consent for the study and for publication of the patients’ anonymous details and images were obtained from the participants or their parents. Clinical studies were performed as described in the Additional file 1. All experimental procedures with laboratory animals were approved by the IRCM Animal Protection Committee and followed guidelines and regulations of the Canadian Council of Animal Care. Whole exome sequencing on DNA from peripheral blood leukocytes was performed using standard methods and as previously described (see Additional file 1 for additional details) [8]. PCR-based Sanger sequencing with fluorescent capillary electrophoresis was performed using standard methods, and results were visualized using Mutation Surveyor (Soft Genetics, Inc.) Numbering in this study refers to Refseq entry NM_001077494.3, equivalent to isoform a, consistent with the usual amino acid numbering in functional studies of NFKB2 protein and the non-canonical processing pathway. Nucleotide numbering for mutations begins at the first A of the initiating methionine ATG.

Lym1 mice were obtained from the Walter and Eliza Hall Institute (Victoria, Australia). Histology and immunohistochemistry were performed as previously described [9]. See Additional file 1 for additional details.

Our original French probands, recruited from different geographic regions through the “Genhypopit” network, [10] were noted to have immunodeficiencies of varying severity with recurrent childhood infections and hypogammaglobulinemia, in addition to ACTH deficiency (patients A1, B1, C1, C2, see Figure 1, our original publication for full clinical details, and Additional file 1 for updated phenotypes; C2 was deceased prior to this study) [1]. Patients A2, A3 and C3 had immune but not endocrine deficiencies. Patient C1 also had a growth hormone deficiency, and this patient has since developed central (i.e. not thyroidal) hypothyroidism. The endocrine deficiencies appear to be longstanding, not acute secondary to current infections. MRI scans of the pituitary were performed for the probands, with hypoplasia observed for patient B1 but not A1 or C1 (Additional file 1: Figure S1A, B, C1, 2). Since the original report, two new carriers were ascertained in family A, A5 and A6 (see below and Additional file 1: Table S1 for clinical details).

We performed whole exome sequencing on DNA from A1, B1 and C1. In the exome data, only one gene carried a rare, protein-altering variant in all three probands, the gene NFKB2 (MIM [164012]). In NFKB2, proband A1 was heterozygous for missense mutation p.A867V, proband B1 was heterozygous for missense mutation p.D865G, and proband C1 was heterozygous for an 8 bp frameshift deletion, p.R853Afs*29 (Figure 1A, B, C). All three mutations were verified by PCR-based Sanger fluorescent sequencing (Additional file 1: Figure S3A-E). Sequencing confirmed that both parents were negative in peripheral blood DNA for the mutations found in the probands in families B and C, as was the one sampled parent in family A. In families A and B, all of the sampled unaffected relatives were negative for the familial mutation (data not shown). Two children of A1, A5 and A6, were heterozygous for the familial mutation (data not shown). One child (A6) showed a clinical phenotype with recurrent infections and immunodeficiency, while A5 had moderate immunoglobulin deficiency with no clinical signs as yet. In family C, affected sibling C3 of the proband also carried the familial mutation. No additional pathogenic variants were found in any of the probands in targeted exons of genes otherwise known to cause hypopituitarism (see Additional file 1). There were rare protein-altering variants detected in several other genes associated with common variable or severe immunodeficiency, some in B1 and some in C1, but none in any two probands together (see Additional file 1: Table S2).

A fourth patient with DAVID syndrome was independently ascertained, previously only reported in abstract form [11]. This patient (D1, see Figure 1, Additional file 1: S2)) was asthenic with immunodeficiency from an early age, suffered hair loss and onychodystrophy, and had low ACTH before and after CRH stimulation. All other pituitary functions were normal, and MRI showed hypoplastic anterior pituitary (Additional file 1: Figure S1D, see Additional file 1 for more details). The two C-terminal coding exons of NFKB2 were directly sequenced in DNA from patient D1, using PCR-based Sanger technology. The patient was heterozygous for a truncating mutation also at amino acid position 853, p.R253X (Figure 1D). This was not the same as the frameshift mutation at the same residue in family C. The mutation was not present in blood DNA from either parent (data not shown).

All variants have been submitted to ClinVar and assigned preliminary accession numbers pending database curation: c.2556_2563del SCV000172085; c.2594A > G SCV000172086; c.2600C > T SCV000172087; c.2557C > T SCV000172088.

Our genetic findings strongly implicate NFKB2 as the causal gene for DAVID syndrome in our patients, particularly taking into account similar mutations found by two other groups in similar patients. In all we identified four different heterozygous mutations, two protein-truncating and two missense variants, near the C-terminus of the protein coding region of NFKB2, a region required for the correct processing of the primary translation product (Figure 1E) [12],[13]. In the three families (B, C, D) where both parents could be sampled, neither parent carried the mutation in DNA from peripheral lymphocytes, consistent with the mutations being germline de novo; family A with only one parent available is also compatible with this model of transmission. The occurrence of two affected siblings in family C is completely consistent with the proposed de novo germ line origin of the mutations. Although uncommon, such mutations may arise in mitotic germ line clones, either in testes or ovaries, and clonally expand, giving rise to mutation-carrying post-meiotic gametes repeatedly, potentially over a period of years. Intrafamilial recurrence in multiple affected siblings with presumptive de novo pre-meiotic germ line origin has been reported in numerous studies of unrelated genetic disorders [14]-[18].

The mutation in family B of Chen et al is identical to the mutation in our family D at the nucleotide and protein level (p.R253X, Figure 1E) [4]. These probably represent independent mutational events, although the ethnicity of their family was not reported. In the family reported by Liu et al., the phenotype co-segregates with a frameshift mutation beginning one amino acid upstream of phosphorylated serine 865. It should be noted that hypoplastic pituitary anatomy as visualized by MRI was not reported by either Chen or Liu et al, whereas two of our patients have anatomical pituitary defects. Similarly, the mutation p.D865G reported by Lee et al is the same as that found in our family B [5]. Lee et al demonstrated that this mutation deleteriously affected NFKB2 protein processing in response to activation of the non-canonical pathway. Oddly, all three patients of Lee et al are reported to have alopecia areata, which we did not observe in our patient B1, whereas their patients lacked an overt endocrine deficiency.

The unique association of ACTH deficiency with combined variable immunodeficiency in DAVID syndrome suggested that NFKB2 might have a critical role in pituitary development, particularly for differentiation of ACTH-producing corticotrope cells. A similar mutation near the C-terminus of the murine Nfkb2 ortholog was previously identified in a large scale mouse mutagenesis screen for cellular immunodeficiency [19]. This Lym1 strain shows severely reduced viability as homozygotes, although heterozygotes are viable and fertile. The mice show multiple aspects of immunodeficiency similar to DAVID syndrome, including a reduced B cell compartment and reduced antigen response with hypogammaglobulinemia and autoimmune response in heterozygotes. However the endocrine status of these mice was not previously described, nor was the anatomical development of their hypothalamus and pituitary. We assessed pituitary development in the Lym1 mice at various ages. Unexpectedly, the gross morphology of pituitaries from wild-type and Lym1heterozygous or homozygous mutant pituitaries did not reveal any obvious anatomical defect either in young mice (Figure 2A, E, I), or in older animals (Additional file 1: Figure S4). Expression of Pomc was assessed using ACTH immunofluorescence, and the differentiation status of corticotropes and melanotropes was assessed using immunostaining for Tpit (Tbx19) Differentiation of anterior lobe corticotropes and intermediate lobe melanotropes appeared normal in distribution and cell numbers (Figure 2C, G, K, S4) and expression of Pomc was not obviously affected by the Lym1 mutation (Figure 2B, F, G, S4). Histological analysis of adrenals from mice of the three genotypes also did not reveal any defect (Figure 2D, H, L). In separate experiments using expression array profiling, we have documented that both Nfkb1 and Nfkb2 are expressed in the adult mouse anterior pituitary (see Additional file 1: Figure S5).

NFκB2 is synthesized as a large precursor (p100) that must be processed to release the active N-terminal rel-homology domain (RHD, p52). Processing of NFκB2 is tightly regulated via the non-canonical pathway, with most protein remaining unprocessed until the cell receives an appropriate signal via receptors such as LTβR, BAFFR, RANK or CD40 [12],[20],[21]. Signalling leads to phosphorylation of specific serines near the C-terminus of NFκB2, including S866 and S870, via a pathway that employs the NIK and IKKA kinases [12]. This in turn leads to cleavage by the proteasome to produce the RHD p52 fragment. The occurrence of missense mutations bracketing S866 in two of our families suggests that these residues are important for recognition of the site by its kinase. Unprocessed full-length NFκB2 p100 can act as an IκB-like inhibitor [12],[22]. Mutations in NFKB2 leading to a protein that is unable to proceed via the non-canonical pathway, but is otherwise functional, are therefore anticipated to generate a dominant negative repressor, which could block the action of multiple RHD-dimers. The tight clustering of six different mutations in human DAVID syndrome patients in the region of NFKB2 regulated by the non-canonical pathway (Figure 1E) is likely related to the specificity and rarity of the phenotype. It should be noted that mutations in NFKB2 have not been reported in cases of isolated anterior pituitary deficiency, lacking an immunodeficiency component.

The pathology we observed in the Lym1 mice is inconsistent with an effect of the Nkfb2 mutation in that strain at the level of primary pituitary development. Alternatively, the human endocrine phenotype might potentially result from an autoimmune reaction, consistent with the observed alopecia in several DAVID patients. Nfkb2 has been shown to play a role in acquired self-tolerance in knockout mice [23]. Mice accumulating p100 as a result of defects in or upstream of Nfkb2 show mild osteopetrosis, [19],[24] and the RHD system is known to play a role in bone metabolism [25]. However, our DAVID syndrome patients carrying NFKB2 mutations have normal bone mineral density (T. Brue and M.-H. Quentien, pers. comm.).

The occurrence of GH and TSH deficiencies in our patient C1, as well as the abnormal pituitary anatomy observed in patients B1 and D1, constitute an expansion of the phenotype for this gene defect. We have previously reported that severe neonatal cortisol deficiency may be associated with transient GH deficiency [26] but an association with TSH deficiency is a novel observation. The subclinical phenotype of patient A5 is indicative of variable penetrance even in the same family, or a possibly delayed onset of the phenotype. The terminology of ‘central adrenal’ deficiency in other papers describing NFKB2 mutations may be overly restrictive, as the endocrine deficiencies among our patients are not restricted to hormones affecting adrenal function. A corticotropin-releasing factor test of patient D1 was consistent with a pituitary and not an hypothalamic defect.

A sixth, French-Canadian patient (F1), was ascertained after being hospitalized at age 17 years for a renal granuloma with immunoglobulin M-complex glomerulonephritis, low IgG levels, and a diagnosis of possible common variable immunodeficiency [27]. She was referred to Endocrinology due to primary amenorrhea and was found to have hypothalamic hypogonadism and severe ACTH and growth hormone deficiencies, although she was not of short stature [28]. Pituitary MRI showed a small anterior pituitary and a thin pituitary stalk with an ectopic posterior pituitary, suggesting a congenital defect [29]. Exome sequencing of DNA from this patient showed no pathogenic variants in NFKB2, whereas all coding exons of the gene were well covered. As is typical, several hundred heterozygous rare potentially pathogenic variants were identified in the complete exome analysis of this patient. The results for patients E1 and F1 suggest that genetic heterogeneity is likely for DAVID syndrome, and that variable endocrine presentation may be a hallmark of the disorder.

The rel homology domain transcription factor signalling pathway is an active target of therapies for cancers especially breast and multiple myeloma [30],[31]. Our results caution that broad inhibition of these pathways, or specific inhibition of NFκB2 processing, could have long-term deleterious effects on central endocrine function. Monitoring endocrine function may be indicated as a component of drug trials targeting NFκB pathways.

We confirm previous findings that mutations near the C-terminus of NFKB2 cause combined endocrine and immunodeficiencies. De novo status of the mutations was confirmed in all cases for which both parents were available. The mutations are consistent with a dominant gain-of-function effect, generating an unprocessed NFKB2 super-repressor protein. We expand the potential phenotype of such NFKB2 mutations to include additional pituitary hormone deficiencies as well as anatomical pituitary anomalies. The lack of an observable endocrine phenotype in Lym1 mice suggests that the endocrine component of DAVID syndrome is either not due to a direct role of NFΚB pathways on pituitary development, or else that human and mouse pituitary development differ in its requirements for NFΚB pathway function.