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Erschienen in: European Spine Journal 8/2017

08.02.2017 | Original Article

Myofibroblast in the ligamentum flavum hypertrophic activity

verfasst von: Junseok W. Hur, Taegeun Bae, Sunghyeok Ye, Joo-Hyun Kim, Sunhye Lee, Kyoungmi Kim, Seung-Hwan Lee, Jin-Soo Kim, Jang-Bo Lee, Tai-Hyoung Cho, Jung-Yul Park, Junho K. Hur

Erschienen in: European Spine Journal | Ausgabe 8/2017

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Abstract

Purpose

Majority of the previous studies compared lumbar spinal stenosis (LSS) and lumbar disc herniation (LDH) patients for analyses of LFH. However, the separation of normal/hypertrophied LF has often been ambiguous and the severity of hypertrophic activity differed. Here, we present a novel analysis scheme for LFH in which myofibroblast is proposed as a major etiological factor for LFH study.

Methods

Seventy-one LF patient tissue samples were used for this study. Initially, mRNA levels of the samples were assessed by qRT-PCR: angiopoietin-like protein-2 (ANGPTL2), transforming growth factor-beta1 (TGF-β1), vascular endothelial growth factor (VEGF), interleukin-6, collagen-1, 3, 4, 5, and 11, and elastin. Myofibroblasts were detected by immune stain using α-smooth muscle actin (αSMA) as a marker. To study the myofibroblast in TGF-β pathway, LF tissues were analyzed for protein levels of αSMA/TGF-β1 by Western blot. In addition, from LF cells cultured with exogenous TGF-β1 conditioned medium, expression of αSMA/collagen-1 was assessed and the cell morphology was identified.

Results

The comparative analysis of mRNA expression levels (LSS vs LDH) failed to show significant differences in TGF-β1 (p = 0.08); however, we found a significant positive correlation among ANGPTL2, VEGF, TGF-β1, and collagen-1 and 3, which represent common trends in hypertrophic activity (p < 0.05). We detected myofibroblast in the patient samples by αSMA staining, and the protein levels of αSMA were positively correlated with TGF-β1. In LF cell culture, exogenous TGF-β1 upregulated αSMA/collagen-1 mRNA levels and facilitated trans-differentiation to myofibroblast.

Conclusions

We conclude that the transition of fibroblast to myofibroblasts via TGF-β pathway is a key linker between inflammation and fibrosis in LFH mechanism.
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Metadaten
Titel
Myofibroblast in the ligamentum flavum hypertrophic activity
verfasst von
Junseok W. Hur
Taegeun Bae
Sunghyeok Ye
Joo-Hyun Kim
Sunhye Lee
Kyoungmi Kim
Seung-Hwan Lee
Jin-Soo Kim
Jang-Bo Lee
Tai-Hyoung Cho
Jung-Yul Park
Junho K. Hur
Publikationsdatum
08.02.2017
Verlag
Springer Berlin Heidelberg
Erschienen in
European Spine Journal / Ausgabe 8/2017
Print ISSN: 0940-6719
Elektronische ISSN: 1432-0932
DOI
https://doi.org/10.1007/s00586-017-4981-2

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