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International Archives of Occupational and Environmental Health

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01.02.2010 | Original Article

Nasal blown secretion evaluation in specific occupational nasal challenges

verfasst von: Patrizia Pignatti, Gianni Pala, Marta Pisati, Luca Perfetti, Gabriella Banchieri, Gianna Moscato

Erschienen in: International Archives of Occupational and Environmental Health | Ausgabe 2/2010

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Abstract

Purpose

To investigate the usefulness of nasal blown secretion evaluation during specific nasal provocation test (sNPT) in diagnosing occupational rhinitis (OR).

Methods

To validate the method, nasal blown secretions from 103 healthy subjects and 30 allergic rhinitis patients were analyzed. Furthermore, we analyzed nasal blown secretions, collected before and after sNPT, of 29 subjects with work-related rhinitis symptoms (WRRS) who underwent the diagnostic pathway for OR. Rhinoscopy and nasal symptom score were used to define a positive sNPT.

Results

A total of 89.6% WRRS subjects provided suitable nasal secretions. Eosinophils significantly increased after positive sNPTs compared to negative ones (P = 0.006). Four percent and/or 1 × 10⁴ eosinophils/ml was the cut-off for a significant post-sNPT eosinophil increase. A total of 4/13 (33%) WRRS subjects with negative sNPT, assessed by rhinoscopy and nasal symptom score, presented a significant post-sNPT nasal eosinophil increase, and were identified as possible OR.

Conclusion

Eosinophil evaluation in nasal blown secretions is an important tool in monitoring the response to occupational specific nasal challenges.

Airaksinen LK, Tuomi TO, Tuppurainen MO et al (2008) Inhalation challenge test in the diagnosis of occupational rhinitis. Am J Rhinol 22:38–46CrossRef

Blaski CA, Watt JL, Quinn TJ et al (1996) Nasal lavage cellularity, grain dust, and airflow obstruction. Chest 109:1086–1092CrossRef

Bousquet J, Khaltaev N, Cruz AA et al (2008) Allergic rhinitis and its impact on asthma (ARIA) 2008 update (in collaboration with the World Health Organization, GA(2)LEN and AllerGen). Allergy 63(suppl 86):8–160CrossRef

Castano R, Thériault G, Maghni K et al (2008) Reproducibility of nasal lavage in the context of the inhalation challenge investigation of occupational rhinitis. Am J Rhinol 22:271–275CrossRef

Castano R, Gautrin D, Thériault G et al (2009) Occupational rhinitis in workers investigated for occupational asthma. Thorax 64:50–54CrossRef

Efthimiadis A, Spanevello A, Hamid Q et al (2002) Methods of sputum processing for cell counts, immunocytochemistry and in situ hybridisation. Eur Respir J Suppl 37:19s–23s

Górski P, Krakowiak A, Ruta U (2000) Nasal and bronchial responses to flour-inhalation in subjects with occupationally induced allergy affecting the airway. Int Arch Occup Environ Health 73:488–497CrossRef

Górski P, Krakowiak A, Pazdrak K et al (1998) Nasal challenge test in the diagnosis of allergic respiratory diseases in subjects occupationally exposed to a high molecular allergen (flour). Occup Med 48:91–97CrossRef

Gosepath J, Amedee RG, Mann WJ (2005) Nasal provocation testing as an international standard for evaluation of allergic and nonallergic rhinitis. Laryngoscope 115:512–516CrossRef

Howarth PH, Persson CGA, Meltzer EO et al (2005) Objective monitoring of nasal airway inflammation in rhinitis. J Allergy Clin Immunol 115:S414–S441CrossRef

Hytönen M, Sala E (1996) Nasal provocation test in the diagnostics of occupational allergic rhinitis. Rhinology 34:86–90

Hytönen M, Leino T, Sala E et al (1997) Nasal provocation test in the diagnostics of hairdressers’ occupational rhinitis. Acta Otolaryngol Suppl 529:133–136CrossRef

Ingels K, Durdurez JP, Cuvelier C et al (1997) Nasal biopsy is superior to nasal smear for finding eosinophils in nonallergic rhinitis. Allergy 52:338–341CrossRef

Kinhult J, Egesten A, Benson M et al (2003) Increased expression of surface markers on neutrophils following migration into nasal lumen. Clin Exp Allergy 33:1141–1146CrossRef

Krakowiak A, Walusiak J, Krawczyk P et al (2008) IL-18 levels in nasal lavage after inhalatory challenge test with flour in bakers diagnosed with occupational asthma. Int J Occup Med Environ Health 9:1–8

Lee HS, Majima Y, Sakakura Y et al (1991) A technique for quantitative cytology of nasal secretions. Eur Arch Otorhinolaryngol 248:406–408CrossRef

Lemière C, Ernst P, Olivenstein R et al (2006) Airway inflammation assessed by invasive and noninvasive means in severe asthma: eosinophilic and noneosinophilic phenotypes. J Allergy Clin Immunol 118:1033–1039CrossRef

Miadonna A, Milazzo N, Gibelli S et al (1999) Nasal response to a single antigen challenge in patients with allergic rhinitic-inflammatory cell recruitment persists up to 48 h. Clin Exp Allergy 29:941–949CrossRef

Moscato G, Pignatti P, Yacoub MR et al (2005) Occupational asthma and occupational rhinitis in hairdressers. Chest 128:3590–3598CrossRef

Moscato G, Vandenplas O, Gerth Van Wijk R et al (2008) Occupational rhinitis. Allergy 63:969–980CrossRef

Moscato G, Vandenplas O, Van Wijk R et al (2009) EAACI Position paper on occupational rhinitis. Respir Res 3:10–16

Nikasinovic-Fournier L, Just J, Seta N et al (2002) Nasal lavage as a tool for the assessment of upper-airway inflammation in adults and children. J Lab Clin Med 139:173–180CrossRef

Pałczyński C, Walusiak J, Ruta U et al (2001) Occupational asthma and rhinitis due to glutaraldehyde: changes in nasal lavage fluid after specific inhalatory challenge test. Allergy 56:1186–1191CrossRef

Pignatti P, Delmastro M, Perfetti L et al (2002) Is dithiothreitol affecting cells and soluble mediators during sputum processing? A modified methodology to process sputum. J Allergy Clin Immunol 110:667–668CrossRef

Prat J, Xaubet A, Mullol J et al (1993) Immunocytologic analysis of nasal cells obtained by nasal lavage: a comparative study with a standard method of cell identification. Allergy 48:587–591CrossRef

Rajakulasingam K (2003) Nasal provocation testing. In: Adkinson NF Jr, Yunginger JW, Busse WW, Bochner BS, Holgate ST, Simons FER (eds) Middleton’s allergy principles and practice. Mosby, Philadelphia, pp 644–655

Raulf-Heimsoth M, Wirtz C, Papenfuss F et al (2000) Nasal lavage mediator profile and cellular composition of nasal brushing material during latex challenge tests. Clin Exp Allergy 30:110–112CrossRef

Roquet A, Ihre E, van-Hage Hamsten M et al (1996) Allergen-induced inflammation in the nose: a comparison of acute and repeated low-dose allergen exposure. Allergy 51:42–48

Vandenplas O, Cartier A, Malo JL (2006) Occupational challenge test. In: Bernstein IL, Chan-Yeung M, Malo JL, Bernstein DI (eds) Asthma in the workplace. Taylor & Francis, New York, pp 227–252

Walusiak J, Wiszniewska M, Krawczyk-Adamus P et al (2004) Occupational allergy to wheat flour. Nasal response to specific inhalative challenge in asthma and rhinitis versus isolated rhinitis: a comparative study. Int J Occup Med Environ Health 17:433–440

Titel: Nasal blown secretion evaluation in specific occupational nasal challenges
verfasst von: Patrizia Pignatti
Gianni Pala
Marta Pisati
Luca Perfetti
Gabriella Banchieri
Gianna Moscato
Publikationsdatum: 01.02.2010
Verlag: Springer-Verlag
Erschienen in: International Archives of Occupational and Environmental Health / Ausgabe 2/2010
Print ISSN: 0340-0131
Elektronische ISSN: 1432-1246
DOI: https://doi.org/10.1007/s00420-009-0459-9

Springer Medizin

Abstract

Purpose

Methods

Results

Conclusion

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