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01.12.2018 | Research article | Ausgabe 1/2018 Open Access

BMC Complementary and Alternative Medicine 1/2018

Neoboutonia melleri var velutina Prain: in vitro and in vivo hepatoprotective effects of the aqueous stem bark extract on acute hepatitis models

Zeitschrift:
BMC Complementary and Alternative Medicine > Ausgabe 1/2018
Autoren:
Anne Marie Endougou Effa, Emilie Gantier, Thierry Hennebelle, Vincent Roumy, Céline Rivière, Théophile Dimo, Pierre Kamtchouing, Pierre Desreumaux, Laurent Dubuquoy
Wichtige Hinweise

Electronic supplementary material

The online version of this article (https://​doi.​org/​10.​1186/​s12906-018-2091-2) contains supplementary material, which is available to authorized users.

Abstract

Background

Hepatitis is a liver inflammation caused by different agents and remains a public health problem worldwide. Medicinal plants are an important source of new molecules being considered for treatment of this disease. Our work aims at evaluating the hepatoprotective properties of Neoboutonia velutina, a Cameroonian medicinal plant.

Methods

The aqueous extract has been prepared using phytochemical methods. HepG2 cells were used to assess anti-inflammatory properties of the extract at different concentrations. Acute hepatitis models (Carbon tetrachloride and Concanavalin A) were performed in mice receiving or not receiving, different extract doses by gavage. Liver injury was assessed using histology, transaminases and pro-inflammatory markers. Extract antioxidant and radical scavenging capacities were evaluated.

Results

The extract led to a significant decrease in pro-inflammatory cytokine expression in vitro and to a remarkable protection of mice from carbon tetrachloride-induced liver injury, as shown by a significant decrease in dose-dependent transaminases level. Upon extract treatment, inflammatory markers were significantly decreased and liver injuries were limited as well. In the Concanavalin A model, the extract displayed weak effects.

Conclusions

Taking into account underlying mechanisms in both hepatitis models, we demonstrate the extract’s radical scavenging capacity. Neoboutonia velutina displays a potent hepatoprotective effect mediated through radical scavenging properties.
Zusatzmaterial
Additional file 1: Figure S1. Pro-inflammatory cytokine expression in Concanavalin A-intoxicated mice. Mice were pretreated with the extract or methylprednisolone and acute liver injury was induced with Concanavalin A intravenous injection after the last treatment. Bar graphs show Tumor Necrosis Factor alpha (A), Interleukin-1 beta (B), Interleukin-6 (C) and Interferon gamma (D) liver expression. Data are expressed as mean ± Standard Error of Mean. Significant Dunn’s post tests are indicated as *p < 0.05; **p < 0.01; ***p < 0.001; ns: non-significant. The p-value indicates the Mann-Whitney test. Two independent experiments; n ≥ 10 in each group. TNFα: Tumor Necrosis Factor alpha; IL-1β: Interleukin-1 beta; IL-6: Interleukin-6; IFNγ: Interferon gamma; NVH: Neoboutonia velutina aqueous extract; MP: Methylprednisolone; ConA: Concanavalin A; GAPDH: Glyceraldehyde-3-Phosphate Dehydrogenase. (TIFF 2650 kb)
12906_2018_2091_MOESM1_ESM.tif
Additional file 2: Figure S2. Lipid peroxidation product and endogen antioxidant activity in Concanavalin A-intoxicated mice. Mice were pretreated with the extract or methylprednisolone and acute liver injury was induced with Concanavalin A intravenous injection after the last treatment. Bar graphs show Malondialdehyde level (A), Glutathione (B), total Superoxide dismutase (C) and Catalase (D) activity in mice. Data are expressed as mean ± Standard Error of the Mean. Significant Dunn’s post tests are indicated as *p < 0.05; **p < 0.01; ***p < 0.001; ns: non-significant. The p-value indicates the Mann-Whitney test. Two independent experiments; n ≥ 10 in each group. MDA: Malondialdehyde, GSH: Glutathione; SOD: Superoxide dismutase; CAT: Catalase; NVH: Neoboutonia velutina aqueous extract; MP: Methylprednisolone (TIFF 1841 kb)
12906_2018_2091_MOESM2_ESM.tif
Additional file 3: Figure S3. NVH TLC for phytochemical analysis. Twenty μl of NVH (50 mg/mL) were deposited on a silica plate which was eluted using water: methanol: acetic acid (12.5:12.5:1). The eluted plate was dried before being soaked for 5 s in the appropriate reagent. A: sulfuric anisaldehyde reagent; B: sulfuric vanillin reagent; C: NEU reagent; D: FeCl3 reagent; E: Dimethylamino-cinnamaldehyde (DMACA) reagent. (TIFF 923 kb)
12906_2018_2091_MOESM3_ESM.tif
Additional file 4: File S4. Raw data from the article. (XLS 569 kb)
12906_2018_2091_MOESM4_ESM.xls
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