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18.02.2017 | Original Article

Novel potential scaffold for periodontal tissue engineering

verfasst von: Raquel Osorio, Camilo Andrés Alfonso-Rodríguez, Estrella Osorio, Antonio L. Medina-Castillo, Miguel Alaminos, Manuel Toledano-Osorio, Manuel Toledano

Erschienen in: Clinical Oral Investigations | Ausgabe 9/2017

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Abstract

Objective

The objective of the study is characterization of novel calcium and zinc-loaded electrospun matrices to be used for periodontal regeneration.

Materials and methods

A polymethylmetacrylate-based membrane was calcium or zinc loaded. Matrices were characterized morphologically by atomic force and scanning electron microscopy and mechanically probed by a nanoindenter. Biomimetic calcium phosphate precipitation on polymeric tissues was assessed. Cell viability tests were performed using oral mucosa fibroblasts. Data were analyzed by Kruskal-Wallis and Mann-Whitney tests or by ANOVA and Student-Newman-Keuls multiple comparisons.

Results

Zinc and calcium loading on matrices did not modify their morphology but increased nanomechanical properties and decreased nanoroughness. Precipitation of calcium and phosphate on the matrix surfaces was observed in zinc-loaded specimens. Matrices were found to be non-toxic to cells in all the assays. Calcium- and zinc-loaded scaffolds presented a very low cytotoxic effect.

Conclusions

Zinc-loaded membranes permit cell viability and promoted mineral precipitation in physiological conditions. Based on the tested nanomechanical properties and scaffold architecture, the proposed membranes may be suitable for cell proliferation.

Clinical relevance

The ability of zinc-loaded matrices to promote precipitation of calcium phosphate deposits, together with their observed non-toxicity and its surface chemistry allowing covalent binding of proteins, may offer new strategies for periodontal regeneration.

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Titel: Novel potential scaffold for periodontal tissue engineering
verfasst von: Raquel Osorio
Camilo Andrés Alfonso-Rodríguez
Estrella Osorio
Antonio L. Medina-Castillo
Miguel Alaminos
Manuel Toledano-Osorio
Manuel Toledano
Publikationsdatum: 18.02.2017
Verlag: Springer Berlin Heidelberg
Erschienen in: Clinical Oral Investigations / Ausgabe 9/2017
Print ISSN: 1432-6981
Elektronische ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-017-2072-8

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Abstract

Objective

Materials and methods

Results

Conclusions

Clinical relevance

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