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Erschienen in: Journal of Assisted Reproduction and Genetics 2/2006

01.02.2006 | Short Communication

Optimization of a Dilution Method for Human Expanded Blastocysts Vitrified Using EM Grids After Artificial Shrinkage

verfasst von: So-Young Lee, Hyung-Jun Kim, Sung-Jin Park, Hye-Jin Yoon, San-Hyun Yoon, Kyung-Hoon Lee, Won-Don Lee, Jin-Ho Lim

Erschienen in: Journal of Assisted Reproduction and Genetics | Ausgabe 2/2006

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Abstract

Purpose: To verify a more effective dilution method that can be applied to human expanded blastocysts that are vitrified after artificial shrinkage.
Methods: Surplus expanded blastocysts that remained after embryo transfer (ET) in in vitro fertilization (IVF) cycles, were cryopreserved. The blastocysts were vitrified on EM grids following artificial shrinkage. After thawing the blastocysts, cryoprotectants were diluted using either a 6- or 2-step method. We examined the survival rate and clinical outcome of blastocysts of 151 patients in our ET program after thawing.
Results: The survival rate of blastocysts that were thawed using a 2-step method (91.6%, 239/261) was comparable with that of the 6-step method (89%, 186/209). The clinical pregnancy rate (45.9%, 39/85) and implantation rate (24.1%, 53/220) were slightly higher in the 2-step method than in the 6-step method (40.9%, 27/66; 19.4%, 33/170).
Conclusions: Our data indicate that the 2-step dilution method could be a simpler and more effective protocol for human expanded blastocysts that are vitrified using EM-grid following artificial shrinkage.
Literatur
1.
Zurück zum Zitat Menezo Y, Nicollet B, Herbaut N, Andre D: Freezing cocultured human blastocysts. Fertil Steril 1992;58:977–980PubMed Menezo Y, Nicollet B, Herbaut N, Andre D: Freezing cocultured human blastocysts. Fertil Steril 1992;58:977–980PubMed
2.
Zurück zum Zitat Kaufman R, Menezo Y, Hazout A, Nicollet B, Dumont M, Servy E: Cocultured blastocysts cryopreservation; experience of more than 500 transfer cycles. Fertil Steril 1995;64:1125–1129PubMed Kaufman R, Menezo Y, Hazout A, Nicollet B, Dumont M, Servy E: Cocultured blastocysts cryopreservation; experience of more than 500 transfer cycles. Fertil Steril 1995;64:1125–1129PubMed
3.
Zurück zum Zitat Yokota Y, Sato S, Yokota M, Ishikawa Y, Makita M, Asada T, Araki Y: Successful pregnancy following blastocyst vitrification. Hum Reprod 2000;15:1802–1803CrossRefPubMed Yokota Y, Sato S, Yokota M, Ishikawa Y, Makita M, Asada T, Araki Y: Successful pregnancy following blastocyst vitrification. Hum Reprod 2000;15:1802–1803CrossRefPubMed
4.
Zurück zum Zitat Hiraoka K, Kiraoka K, Kinutani M, Kinutani K: Blastocoele collapse by micropipetting prior to vitrification gives excellent survival and pregnancy outcomes for human day 5 and 6 expanded blastocysts. Hum Reprod 2004;19:2884–2888CrossRefPubMed Hiraoka K, Kiraoka K, Kinutani M, Kinutani K: Blastocoele collapse by micropipetting prior to vitrification gives excellent survival and pregnancy outcomes for human day 5 and 6 expanded blastocysts. Hum Reprod 2004;19:2884–2888CrossRefPubMed
5.
Zurück zum Zitat Mukaida T, Nakanura S, Tomiyama T, Wada S, Kasai M, Takahashi K: Successful birth after transfer of vitrified human blastocysts with use of a cryoloop containerless technique. Fertil Steril 2001;76:618–620CrossRefPubMed Mukaida T, Nakanura S, Tomiyama T, Wada S, Kasai M, Takahashi K: Successful birth after transfer of vitrified human blastocysts with use of a cryoloop containerless technique. Fertil Steril 2001;76:618–620CrossRefPubMed
6.
Zurück zum Zitat Mukaida T, Nakamura S, Tomiyama T, Wada S, Oka C, Kasai M, Takahashi K: Vitrification of human blastocysts using cryoloops: Clinical outcome of 223 cycle. Hum Reprod 2003;18:384–391CrossRefPubMed Mukaida T, Nakamura S, Tomiyama T, Wada S, Oka C, Kasai M, Takahashi K: Vitrification of human blastocysts using cryoloops: Clinical outcome of 223 cycle. Hum Reprod 2003;18:384–391CrossRefPubMed
7.
Zurück zum Zitat Reed ML, Lane M, Gardner DK, Jensen NL, Thompson J: Vitrification of human blastocysts using the cryoloop method: Successful clinical application and birth of offspring. J Assist Reprod Genet 2002;6:304–306CrossRef Reed ML, Lane M, Gardner DK, Jensen NL, Thompson J: Vitrification of human blastocysts using the cryoloop method: Successful clinical application and birth of offspring. J Assist Reprod Genet 2002;6:304–306CrossRef
8.
Zurück zum Zitat Choi DH, Chung HM, Lim JM, Ko JJ, Yoon TK, Cha KY: Pregnancy and delivery of healthy infants developed from vitrified blastocysts in an IVF-ET program. Fertil Steril 2000;74:838–844CrossRefPubMed Choi DH, Chung HM, Lim JM, Ko JJ, Yoon TK, Cha KY: Pregnancy and delivery of healthy infants developed from vitrified blastocysts in an IVF-ET program. Fertil Steril 2000;74:838–844CrossRefPubMed
9.
Zurück zum Zitat Cho HJ, Son WY, Yoon SH, Lee SW, Lim JH: An improved protocol for dilution of cryoprotectants from vitrified human blastocysts. Hum Reprod 2002;17:2419–2422PubMedCrossRef Cho HJ, Son WY, Yoon SH, Lee SW, Lim JH: An improved protocol for dilution of cryoprotectants from vitrified human blastocysts. Hum Reprod 2002;17:2419–2422PubMedCrossRef
10.
Zurück zum Zitat Son WY, Yoon SH, Yoon HJ, Lee SM, Lim JH: Pregnancy outcome following transfer of human blastocysts vitrified on electron microscopy grids after induced collapse of the blastocoele. Hum Reprod 2003;18:137–139CrossRefPubMed Son WY, Yoon SH, Yoon HJ, Lee SM, Lim JH: Pregnancy outcome following transfer of human blastocysts vitrified on electron microscopy grids after induced collapse of the blastocoele. Hum Reprod 2003;18:137–139CrossRefPubMed
11.
Zurück zum Zitat Vanderzwalmen P, Bertin G, Debauche CH, Standaert V, van Roosendaal E, Vandervorst M, Schoysman R, Zech N: Vitrification of human blastocysts with the Hemi-Straw carrier: Application of assisted hatching after thawing. Hum Reprod 2003;18:1504–1511CrossRefPubMed Vanderzwalmen P, Bertin G, Debauche CH, Standaert V, van Roosendaal E, Vandervorst M, Schoysman R, Zech N: Vitrification of human blastocysts with the Hemi-Straw carrier: Application of assisted hatching after thawing. Hum Reprod 2003;18:1504–1511CrossRefPubMed
12.
Zurück zum Zitat Vanderzwalmen P, Bertin G, Debauche CH, Standaert V, van Roosendaal E, Vandervorst M, Bollen N, Zech H, Mukaida T, Takahashi K, Schoysman R: Births after vitrification at morula and blastocyst stage: Effect of artificial reduction of the blastocoeleic cavity before vitrification. Hum Reprod 2002;17:744–751CrossRefPubMed Vanderzwalmen P, Bertin G, Debauche CH, Standaert V, van Roosendaal E, Vandervorst M, Bollen N, Zech H, Mukaida T, Takahashi K, Schoysman R: Births after vitrification at morula and blastocyst stage: Effect of artificial reduction of the blastocoeleic cavity before vitrification. Hum Reprod 2002;17:744–751CrossRefPubMed
13.
Zurück zum Zitat Yoon HG, Yoon SH, Son WY, Kim JG, Im KS, Lim JH: Alternative embryo transfer of day 3 or day 5 for reducing the risk of multiple gestations. J Assist Reprod Genet 2001;18:262–267CrossRefPubMed Yoon HG, Yoon SH, Son WY, Kim JG, Im KS, Lim JH: Alternative embryo transfer of day 3 or day 5 for reducing the risk of multiple gestations. J Assist Reprod Genet 2001;18:262–267CrossRefPubMed
14.
Zurück zum Zitat Kasai M, Komi JH, Takakamo A, Tsudera H, Sakurai T, Machida T: A simple method for mouse embryo cryopreservation in a low toxicity vitrification solution, without appreciable loss of viability. J Reprod Fertil 1990;89:91–97PubMed Kasai M, Komi JH, Takakamo A, Tsudera H, Sakurai T, Machida T: A simple method for mouse embryo cryopreservation in a low toxicity vitrification solution, without appreciable loss of viability. J Reprod Fertil 1990;89:91–97PubMed
15.
Zurück zum Zitat Kuwayama M, Hamano S, Nagai T: Vitrification of bovine blastocysts obtained by in vitro culture of oocytes matured and fertilized in vitro. J Reprod Fertil 1992;96:187–193PubMed Kuwayama M, Hamano S, Nagai T: Vitrification of bovine blastocysts obtained by in vitro culture of oocytes matured and fertilized in vitro. J Reprod Fertil 1992;96:187–193PubMed
16.
Zurück zum Zitat Isachenko V, Montag M, Isachenko E, Nawroth F, Dessole S, van der Ven H: Developmental rate and ultrastructure of vitrified human pronuclear oocytes after step-wise versus direct rehydration. Hum Reprod 2004;19:660–665CrossRefPubMed Isachenko V, Montag M, Isachenko E, Nawroth F, Dessole S, van der Ven H: Developmental rate and ultrastructure of vitrified human pronuclear oocytes after step-wise versus direct rehydration. Hum Reprod 2004;19:660–665CrossRefPubMed
17.
Zurück zum Zitat Takahashi K, Kukaida T, Goto T, Oka C: Perinatal outcome of blastocyst transfer with vitrification using cryoloop: A 4-year follow-up study. Fertil Steril 2004;84:88–92CrossRef Takahashi K, Kukaida T, Goto T, Oka C: Perinatal outcome of blastocyst transfer with vitrification using cryoloop: A 4-year follow-up study. Fertil Steril 2004;84:88–92CrossRef
Metadaten
Titel
Optimization of a Dilution Method for Human Expanded Blastocysts Vitrified Using EM Grids After Artificial Shrinkage
verfasst von
So-Young Lee
Hyung-Jun Kim
Sung-Jin Park
Hye-Jin Yoon
San-Hyun Yoon
Kyung-Hoon Lee
Won-Don Lee
Jin-Ho Lim
Publikationsdatum
01.02.2006
Verlag
Springer US
Erschienen in
Journal of Assisted Reproduction and Genetics / Ausgabe 2/2006
Print ISSN: 1058-0468
Elektronische ISSN: 1573-7330
DOI
https://doi.org/10.1007/s10815-005-9006-0

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