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Medical Oncology

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01.06.2010 | Original Paper

Overexpression of FADD enhances 5-fluorouracil-induced apoptosis in colorectal adenocarcinoma cells

verfasst von: Anning Yin, Yingan Jiang, Xianfeng Zhang, Hesheng Luo

Erschienen in: Medical Oncology | Ausgabe 2/2010

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Abstract

To investigate the mechanism of enhancing apoptosis-inducing effects of 5-fluorouracil on human colorectal adenocarcinoma cells by stable transfection of extrinsic Fas-associated death domain protein (FADD) gene, both in vitro and in vivo. FADD gene of stable overexpression was determined by reverse transcription polymerase chain reaction (RT-PCR) assay and Western blotting assay. After treatment with 5-fluorouracil as an apoptotic inducer, in vitro cell growth activities were investigated by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Cell apoptosis and its rates were evaluated by TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling) assay and flow cytometry of annexin V-FITC/PI staining. To examine the combination therapeutic effect of FADD and 5-fluorouracil, tumor xenograft model was prepared for in vivo study. Compared with SW480 and SW480/neo cells, FADD mRNA and protein levels of SW480/FADD cells were higher. Chemosensitivity and apoptosis rates of SW480/FADD cells were remarkably higher than SW480 and SW480/neo cells when treated with 5-fluorouracil. In in vivo study, overexpression of FADD increased the efficacy of 5-fluorouracil-induced inhibition of tumor growth in nude mice. Stable overexpression of extrinsic FADD gene can conspicuously ameliorate apoptosis-inducing effects of 5-fluorouracil on colorectal adenocarcinoma cells, which is a novel strategy to improve chemotherapeutic effects on colorectal cancer.

Etzioni DA, El-Khoueiry AB, Beart RW Jr. Rates and predictors of chemotherapy use for stage III colon cancer: a systematic review. Cancer. 2008;113(12):3279–89. doi:10.1002/cncr.23958.CrossRefPubMed

Jemal A, Siegel R, Ward E, Hao Y, Xu J, et al. Cancer statistics, 2008. CA Cancer J Clin. 2008;58(2):71–96. doi:10.3322/CA.2007.0010.CrossRefPubMed

Lim YJ, Rhee JC, Bae YM, Chun WJ. Celecoxib attenuates 5-fluorouracil-induced apoptosis in HCT-15 and HT-29 human colon cancer cells. World J Gastroenterol. 2007;13(13):1947–52.PubMed

Pinedo HM, Peters GF. 5-Fluorouracil: biochemistry and pharmacology. J Clin Oncol. 1988;6(10):1653–64.PubMed

Sargent DJ, Goldberg RM, Jacobson SD, Macdonald JS, Labianca R, et al. A pool-ed analysis of adjuvant chemotherapy for resected colon cancer in elderly patients. N Engl J Med. 2001;345(15):1091–7. doi:10.1056/NEJMoa010957.CrossRefPubMed

Chinnaiyan AM, O’Rourke K, Tewari M, Dixit VM. FADD, a novel death domain-containing protein, interacts with the death domain of Fas and initiates apoptosis. Cell. 1995;81(4):505–12. doi:10.1016/0092-8674(95)90071-3.CrossRefPubMed

Boldin MP, Mett IL, Varfolomeev EE, Chumakov I, Shemer-Avni Y, et al. Self-association of the “death domains” of the p55 tumor necrosis factor (TNF) receptor and Fas/APO1 prompts signaling for TNF and Fas/APO1 effects. J Biol Chem. 1995;270(1):387–91. doi:10.1074/jbc.270.1.387.CrossRefPubMed

Kim PK, Dutra AS, Chandrasekharappa SC, Puck JM. Genomic structure and map-ing of human FADD, an intracellular mediator of lymphocyte apoptosis. J Immunol. 1996;157(12):5461–6.PubMed

Thorburn A. Death receptor-induced cell killing. Cell Signal. 2004;16(2):139–44. doi:10.1016/j.cellsig.2003.08.007.CrossRefPubMed

10.

Kondo S, Ishizaka Y, Okada T, Kondo Y, Hitomi M, et al. FADD gene therapy for malignant gliomas in vitro and in vivo. Hum Gene Ther. 1998;9(11):1599–608. doi:10.1089/hum.1998.9.11-1599.CrossRefPubMed

11.

Kobayashi T, Okamoto K, Kobata T, Hasunuma T, Kato T, et al. Novel gene therapy for rheumatoid arthritis by FADD gene transfer: induction of apoptosis of rheumatoid synoviocytes but not chondrocytes. Gene Ther. 2000;7(6):527–33. doi:10.1038/sj.gt.3301127.CrossRefPubMed

12.

Yoshikawa R, Kusunoki M, Yanagi H, Noda M, Furuyama JI, et al. Dual antitumor effects of 5-fluorouracil on the cell cycle in colorectal carcinoma cells: a novel target mechanism concept for pharmacokinetic modulating chemotherapy. Cancer Res. 2001;61(3):1029–37.PubMed

13.

Moertel CG, Fleming TR, Macdonald JS, Haller DG, Laurie JA, et al. Levamisole and fluorouracil for adjuvant therapy of resected colon carcinoma. N Engl J Med. 1990;322(6):352–8.PubMedCrossRef

14.

Benson AB 3rd. New approaches to assessing and treating early-stage colon and rectal cancers: cooperative group strategies for assessing optimal approaches in early-stage disease. Clin Cancer Res. 2007;13(22):6913–20. doi:10.1158/1078-0432.CCR-07-1188.CrossRef

15.

Andre N, Schmiegel W. Chemoradiotherapy for colorectal cancer. Gut. 2005;54(8):1194–202. doi:10.1136/gut.2004.062745.CrossRefPubMed

16.

André T, Boni C, Mounedji-Boudiaf L, Navarro M, Tabernero J, et al. Oxaliplatin, fluorouracil, and leucovorin as adjuvant treatment for colon cancer. N Engl J Med. 2004;350(23):2343–51. doi:10.1056/NEJMoa032709.CrossRefPubMed

17.

Lecomte T, Ferraz JM, Zinzindohoué F, Loriot MA, Tregouet DA, et al. Thymidylate synthase gene polymorphism predicts toxicity in colorectal cancer patients receiving 5-fluorouracil-based chemotherapy. Clin Cancer Res. 2004;10(17):5880–8. doi:10.1158/1078-0432.CCR-04-0169.CrossRefPubMed

18.

Fesik SW. Promoting apoptosis as a strategy for cancer drug discovery. Nat Rev Cancer. 2005;5(11):876–85. doi:10.1038/nrc1736.CrossRefPubMed

19.

Holler N, Zaru R, Micheau O, Thome M, Attinger A, et al. Fas triggers an alternative, caspase-8-independent cell death pathway using the kinase RIP as effector molecule. Nat Immunol. 2000;1(6):489–95. doi:10.1038/82732.CrossRefPubMed

20.

Imai T, Adachi S, Nishijo K, Ohgushi M, Okada M, et al. FR901228 induces tumor regression associated with induction of Fas ligand and activation of Fas signaling in human osteosarcoma cells. Oncogene. 2003;22(58):9231–42. doi:10.1038/sj.onc.1207184.CrossRefPubMed

21.

Shimada K, Nakamura M, Ishida E, Kishi M, Yonehara S, et al. Phosphorylation of Fas-associated death domain contributes to enhancement of etoposide-induced apoptosis in prostate cancer cells. Jpn J Cancer Res. 2002;93(10):1164–74.PubMed

22.

Keiji S, Syuichi M, Mitsutoshi N, Eiwa I, Munehiro K, et al. Phosphorylation of FADD is critical for sensitivity to anticancer drug-induced apoptosis. Carcinogenesis. 2004;25(7):1089–97. doi:10.1093/carcin/bgh130.CrossRef

23.

Micheau O, Solary E, Hammann A, Dimanche-Boitrel MT. Fas ligand-independent, FADD-mediated activation of the Fas death pathway by anticancer drugs. J. Biol. Chem. 1999;274(12):7987–92. doi:10.1074/jbc.274.12.7987.CrossRefPubMed

24.

Boldin MP, Goncharov TM, Goltsev YV, Wallach D. Involvement of MACH, a novel MORT1/FADD-interacting protease, in Fas/APO-1- and TNF receptor-induced cell death. Cell. 1996;85(6):803–15. doi:10.1016/S0092-8674(00)81265-9.CrossRefPubMed

25.

Muzio M, Chinnaiyan AM, Kischkel FC, O’Rourke K, Shevchenko A, et al. FLICE, a novel FADD-homologous ICE/CED-3-like protease, is recruited to the CD95 (Fas/APO-1) death–inducing signaling complex. Cell. 1996;85(6):817–27. doi:10.1016/S0092-8674(00)81266-0.CrossRefPubMed

26.

Hirata H, Takahashi A, Kobayashi S, Yonehara S, Sawai H, et al. Caspases are activated in a branched protease cascade and control distinct downstream processes in Fas-induced apoptosis. J Exp Med. 1998;187(4):587–600. doi:10.1084/jem.187.4.587.CrossRefPubMed

27.

Astrin SM. Are there molecular targets for therapy of colon cancer? Oncology (Williston Park). 1991;5(10):127–32.

28.

Sabaawy HE, Farley T, Ahmed T, Feldman E, Abraham NG. Synergetic effects of retrovirus IFN-alpha gene transfer and 5-FU on apoptosis of colon cancer cells. Acta Haematol. 1999;101(2):82–8. doi:10.1159/000040929.CrossRefPubMed

29.

Durai R, Yang SY, Seifalian AM, Winslet MC. Principles and applications of gene therapy in colon cancer. J Gastrointestin Liver Dis. 2008;17(1):59–67.PubMed

30.

Dong F, Wang L, Davis JJ, Hu W, Zhang L, et al. Eliminating established tumor in nu/nu nude mice by a tumor necrosis factor-alpha-related apoptosis-inducing ligand-armed oncolytic adenovirus. Clin Cancer Res. 2006;12(17):5224–30. doi:10.1158/1078-0432.CCR-06-0244.CrossRefPubMed

31.

Lipinski KS, Djeha AH, Ismail T, Mountain A, Young LS, et al. High-level, beta-catenin/TCF-dependent transgene expression in secondary colorectal cancer tissue. Mol Ther. 2001;4(4):365–71. doi:10.1006/mthe.2001.0468.CrossRefPubMed

32.

Choi EA, Lei H, Maron DJ, Mick R, Barsoum J, et al. Combined 5-fluorouracil/systemic interferon-beta gene therapy results in long-term survival in mice with established colorectal liver metastases. Clin Cancer Res. 2004;10(4):1535–44. doi:10.1158/1078-0432.CCR-0040-03.CrossRefPubMed

33.

Li H, Zhu H, Xu CJ, Yuan J. Cleavage of BID by caspase 8 mediates the mitochondrial damage in the Fas pathway of apoptosis. Cell. 1998;94(3):491–501. doi:10.1016/S0092-8674(00)81590-1.CrossRefPubMed

Titel: Overexpression of FADD enhances 5-fluorouracil-induced apoptosis in colorectal adenocarcinoma cells
verfasst von: Anning Yin
Yingan Jiang
Xianfeng Zhang
Hesheng Luo
Publikationsdatum: 01.06.2010
Verlag: Springer US
Erschienen in: Medical Oncology / Ausgabe 2/2010
Print ISSN: 1357-0560
Elektronische ISSN: 1559-131X
DOI: https://doi.org/10.1007/s12032-009-9224-x

Springer Medizin

Overexpression of FADD enhances 5-fluorouracil-induced apoptosis in colorectal adenocarcinoma cells

Abstract

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