Skip to main content
Erschienen in: Archives of Virology 9/2008

01.09.2008 | Brief Report

Peanut stunt virus 2b cistron plays a role in viral local and systemic accumulation and virulence in Nicotiana benthamiana

verfasst von: O. Netsu, K. Hiratsuka, S. Kuwata, T. Hibi, M. Ugaki, M. Suzuki

Erschienen in: Archives of Virology | Ausgabe 9/2008

Einloggen, um Zugang zu erhalten

Abstract

To analyze the role of the 2b protein (2bP) of Peanut stunt virus (PSV) in the viral infection cycle, we constructed PSV mutants that express either no 2bP or N-terminal-truncated 2bP. The accumulation of wild-type and mutant viruses in tobacco protoplasts indicated that the 2b cistron is not essential for viral replication. Viral accumulation in Nicotiana benthamiana plants suggested that the 2b cistron is responsible for viral accumulation in inoculated and upper leaves and has a role in virulence. The involvement of eight N-terminal amino acids of 2bP in these functions is discussed.
Literatur
1.
Zurück zum Zitat Brigneti G, Voinnet O, Li WX, Ji LH, Ding SW, Baulcombe DC (1998) Viral pathogenicity determinants are suppressors of transgene silencing in Nicotiana benthamiana. EMBO J 17:6739–6746PubMedCrossRef Brigneti G, Voinnet O, Li WX, Ji LH, Ding SW, Baulcombe DC (1998) Viral pathogenicity determinants are suppressors of transgene silencing in Nicotiana benthamiana. EMBO J 17:6739–6746PubMedCrossRef
2.
Zurück zum Zitat Ding SW, Anderson BJ, Haase HR, Symons RH (1994) New overlapping gene encoded by the cucumber mosaic virus genome. Virology 198:593–601PubMedCrossRef Ding SW, Anderson BJ, Haase HR, Symons RH (1994) New overlapping gene encoded by the cucumber mosaic virus genome. Virology 198:593–601PubMedCrossRef
3.
Zurück zum Zitat Ding SW, Li WX, Symons RH (1995) A novel naturally occurring hybrid gene encoded by a plant RNA virus facilitates long distance virus movement. EMBO J 14:5762–5772PubMed Ding SW, Li WX, Symons RH (1995) A novel naturally occurring hybrid gene encoded by a plant RNA virus facilitates long distance virus movement. EMBO J 14:5762–5772PubMed
4.
Zurück zum Zitat Ding SW, Shi BJ, Li WX, Symons RH (1996) An interspecies hybrid RNA virus is significantly more virulent than either parental virus. Proc Natl Acad Sci USA 93:7470–7474PubMedCrossRef Ding SW, Shi BJ, Li WX, Symons RH (1996) An interspecies hybrid RNA virus is significantly more virulent than either parental virus. Proc Natl Acad Sci USA 93:7470–7474PubMedCrossRef
5.
Zurück zum Zitat Hayes RJ, Buck KW (1990) Complete replication of a eukaryotic virus RNA in vitro by a purified RNA-dependent RNA polymerase. Cell 63:363–368PubMedCrossRef Hayes RJ, Buck KW (1990) Complete replication of a eukaryotic virus RNA in vitro by a purified RNA-dependent RNA polymerase. Cell 63:363–368PubMedCrossRef
6.
Zurück zum Zitat Hu CC, Aboul-Ata AE, Naidu RA, Ghabrial SA (1997) Evidence for the occurrence of two distinct subgroups of peanut stunt cucumovirus strains: molecular characterization of RNA 3. J Gen Virol 78:929–939PubMed Hu CC, Aboul-Ata AE, Naidu RA, Ghabrial SA (1997) Evidence for the occurrence of two distinct subgroups of peanut stunt cucumovirus strains: molecular characterization of RNA 3. J Gen Virol 78:929–939PubMed
7.
Zurück zum Zitat Hu CC, Sanger M, Ghabrial SA (1998) Production of infectious RNA transcripts from full-length cDNA clones representing two subgroups of peanut stunt virus strains: mapping satellite RNA support to RNA 1. J Gen Virol 79:2013–2021PubMed Hu CC, Sanger M, Ghabrial SA (1998) Production of infectious RNA transcripts from full-length cDNA clones representing two subgroups of peanut stunt virus strains: mapping satellite RNA support to RNA 1. J Gen Virol 79:2013–2021PubMed
8.
Zurück zum Zitat Karasawa A, Nakaho K, Kakutani T, Minobe Y, Ehara Y (1991) Nucleotide sequence of RNA 3 of peanut stunt cucumovirus. Virology 185:464–467PubMedCrossRef Karasawa A, Nakaho K, Kakutani T, Minobe Y, Ehara Y (1991) Nucleotide sequence of RNA 3 of peanut stunt cucumovirus. Virology 185:464–467PubMedCrossRef
9.
Zurück zum Zitat Karasawa A, Nakaho K, Kakutani T, Minobe Y, Ehara Y (1992) Nucleotide sequence analysis of peanut stunt cucumovirus RNAs 1 and 2. J Gen Virol 73:701–707PubMedCrossRef Karasawa A, Nakaho K, Kakutani T, Minobe Y, Ehara Y (1992) Nucleotide sequence analysis of peanut stunt cucumovirus RNAs 1 and 2. J Gen Virol 73:701–707PubMedCrossRef
10.
Zurück zum Zitat Lu R, Folimonov A, Shintaku M, Li WX, Falk BW, Dawson WO, Ding SW (2004) Three distinct suppressors of RNA silencing encoded by a 20-kb viral RNA genome. Proc Natl Acad Sci USA 101:15742–15747PubMedCrossRef Lu R, Folimonov A, Shintaku M, Li WX, Falk BW, Dawson WO, Ding SW (2004) Three distinct suppressors of RNA silencing encoded by a 20-kb viral RNA genome. Proc Natl Acad Sci USA 101:15742–15747PubMedCrossRef
11.
Zurück zum Zitat Lucy AP, Guo HS, Li WX, Ding SW (2000) Suppression of post-transcriptional gene silencing by a plant viral protein localized in the nucleus. EMBO J 19:1672–1680PubMedCrossRef Lucy AP, Guo HS, Li WX, Ding SW (2000) Suppression of post-transcriptional gene silencing by a plant viral protein localized in the nucleus. EMBO J 19:1672–1680PubMedCrossRef
12.
Zurück zum Zitat Nitta N, Takanami Y, Kuwata S, Kubo S (1988) Inoculation with RNAs 1 and 2 of cucumber mosaic virus induces viral RNA replicase activity in tobacco mesophyll protoplasts. J Gen Virol 69:2695–2700CrossRef Nitta N, Takanami Y, Kuwata S, Kubo S (1988) Inoculation with RNAs 1 and 2 of cucumber mosaic virus induces viral RNA replicase activity in tobacco mesophyll protoplasts. J Gen Virol 69:2695–2700CrossRef
14.
Zurück zum Zitat Shi BJ, Ding SW, Symons RH (1997) In vivo expression of an overlapping gene encoded by the cucumoviruses. J Gen Virol 78:237–241PubMed Shi BJ, Ding SW, Symons RH (1997) In vivo expression of an overlapping gene encoded by the cucumoviruses. J Gen Virol 78:237–241PubMed
15.
Zurück zum Zitat Sivakumaran K, Chen MH, Roossinck MJ, Kao CC (2002) Core promoter for initiation of Cucumber mosaic virus subgenomic RNA 4A. Mol Plant Pathol 3:43–52CrossRef Sivakumaran K, Chen MH, Roossinck MJ, Kao CC (2002) Core promoter for initiation of Cucumber mosaic virus subgenomic RNA 4A. Mol Plant Pathol 3:43–52CrossRef
16.
Zurück zum Zitat Soards AJ, Murphy AM, Palukaitis P, Carr JP (2002) Virulence and differential local and systemic spread of Cucumber mosaic virus in tobacco are affected by the CMV 2b protein. Mol Plant Microbe Interact 15:647–653PubMedCrossRef Soards AJ, Murphy AM, Palukaitis P, Carr JP (2002) Virulence and differential local and systemic spread of Cucumber mosaic virus in tobacco are affected by the CMV 2b protein. Mol Plant Microbe Interact 15:647–653PubMedCrossRef
17.
Zurück zum Zitat Suzuki M, Kuwata S, Kataoka J, Masuta C, Nitta N, Takanami Y (1991) Functional analysis of deletion mutants of cucumber mosaic virus RNA 3 using an in vitro transcription system. Virology 183:106–113PubMedCrossRef Suzuki M, Kuwata S, Kataoka J, Masuta C, Nitta N, Takanami Y (1991) Functional analysis of deletion mutants of cucumber mosaic virus RNA 3 using an in vitro transcription system. Virology 183:106–113PubMedCrossRef
18.
Zurück zum Zitat Suzuki M, Kuwata S, Masuta C, Takanami Y (1995) Point mutations in the coat protein of cucumber mosaic virus affect symptom expression and virion accumulation in tobacco. J Gen Virol 76:1791–1799PubMedCrossRef Suzuki M, Kuwata S, Masuta C, Takanami Y (1995) Point mutations in the coat protein of cucumber mosaic virus affect symptom expression and virion accumulation in tobacco. J Gen Virol 76:1791–1799PubMedCrossRef
19.
Zurück zum Zitat Suzuki M, Yoshida M, Yoshinuma T, Hibi T (2003) Interaction of replicase components between Cucumber mosaic virus and Peanut stunt virus. J Gen Virol 84:1931–1939PubMedCrossRef Suzuki M, Yoshida M, Yoshinuma T, Hibi T (2003) Interaction of replicase components between Cucumber mosaic virus and Peanut stunt virus. J Gen Virol 84:1931–1939PubMedCrossRef
20.
Zurück zum Zitat Takeshita M, Suzuki M, Kuwata S, Takanami Y (1998) Involvement of cucumber mosaic virus RNA 2 and RNA 3 in viral systemic spread in radish plant. Arch Virol 143:1109–1117PubMedCrossRef Takeshita M, Suzuki M, Kuwata S, Takanami Y (1998) Involvement of cucumber mosaic virus RNA 2 and RNA 3 in viral systemic spread in radish plant. Arch Virol 143:1109–1117PubMedCrossRef
21.
Zurück zum Zitat Takeshita M, Suzuki M, Takanami Y (2001) Combination of amino acids in the 3a protein and the coat protein of Cucumber mosaic virus determines symptom expression and viral spread in bottle gourd. Arch Virol 146:697–711PubMedCrossRef Takeshita M, Suzuki M, Takanami Y (2001) Combination of amino acids in the 3a protein and the coat protein of Cucumber mosaic virus determines symptom expression and viral spread in bottle gourd. Arch Virol 146:697–711PubMedCrossRef
22.
Zurück zum Zitat Tsuchizaki T (1973) Peanut stunt virus isolated from beans in Japan. Ann Phytopath Soc Jpn 39:67–72 Tsuchizaki T (1973) Peanut stunt virus isolated from beans in Japan. Ann Phytopath Soc Jpn 39:67–72
23.
Zurück zum Zitat Yan LY, Xu ZY, Goldbach R, Kunrong C, Prins M (2005) Nucleotide sequence analyses of genomic RNAs of Peanut stunt virus Mi, the type strain representative of a novel PSV subgroup from China. Arch Virol 150:1203–1211PubMedCrossRef Yan LY, Xu ZY, Goldbach R, Kunrong C, Prins M (2005) Nucleotide sequence analyses of genomic RNAs of Peanut stunt virus Mi, the type strain representative of a novel PSV subgroup from China. Arch Virol 150:1203–1211PubMedCrossRef
24.
Zurück zum Zitat Zhang X, Yuan YR, Pei Y, Lin SS, Tuschl T, Patel DJ, Chua NH (2006) Cucumber mosaic virus-encoded 2b suppressor inhibits Arabidopsis Argonaute1 cleavage activity to counter plant defense. Genes Dev 20:3255–3268PubMedCrossRef Zhang X, Yuan YR, Pei Y, Lin SS, Tuschl T, Patel DJ, Chua NH (2006) Cucumber mosaic virus-encoded 2b suppressor inhibits Arabidopsis Argonaute1 cleavage activity to counter plant defense. Genes Dev 20:3255–3268PubMedCrossRef
25.
Zurück zum Zitat Zuker M (2003) Mfold web server for nucleic acid folding and hybridization prediction. Nucl Acids Res 31:3406–3415PubMedCrossRef Zuker M (2003) Mfold web server for nucleic acid folding and hybridization prediction. Nucl Acids Res 31:3406–3415PubMedCrossRef
Metadaten
Titel
Peanut stunt virus 2b cistron plays a role in viral local and systemic accumulation and virulence in Nicotiana benthamiana
verfasst von
O. Netsu
K. Hiratsuka
S. Kuwata
T. Hibi
M. Ugaki
M. Suzuki
Publikationsdatum
01.09.2008
Verlag
Springer Vienna
Erschienen in
Archives of Virology / Ausgabe 9/2008
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-008-0166-y

Weitere Artikel der Ausgabe 9/2008

Archives of Virology 9/2008 Zur Ausgabe

Leitlinien kompakt für die Innere Medizin

Mit medbee Pocketcards sicher entscheiden.

Seit 2022 gehört die medbee GmbH zum Springer Medizin Verlag

Echinokokkose medikamentös behandeln oder operieren?

06.05.2024 DCK 2024 Kongressbericht

Die Therapie von Echinokokkosen sollte immer in spezialisierten Zentren erfolgen. Eine symptomlose Echinokokkose kann – egal ob von Hunde- oder Fuchsbandwurm ausgelöst – konservativ erfolgen. Wenn eine Op. nötig ist, kann es sinnvoll sein, vorher Zysten zu leeren und zu desinfizieren. 

Umsetzung der POMGAT-Leitlinie läuft

03.05.2024 DCK 2024 Kongressbericht

Seit November 2023 gibt es evidenzbasierte Empfehlungen zum perioperativen Management bei gastrointestinalen Tumoren (POMGAT) auf S3-Niveau. Vieles wird schon entsprechend der Empfehlungen durchgeführt. Wo es im Alltag noch hapert, zeigt eine Umfrage in einem Klinikverbund.

Proximale Humerusfraktur: Auch 100-Jährige operieren?

01.05.2024 DCK 2024 Kongressbericht

Mit dem demographischen Wandel versorgt auch die Chirurgie immer mehr betagte Menschen. Von Entwicklungen wie Fast-Track können auch ältere Menschen profitieren und bei proximaler Humerusfraktur können selbst manche 100-Jährige noch sicher operiert werden.

Die „Zehn Gebote“ des Endokarditis-Managements

30.04.2024 Endokarditis Leitlinie kompakt

Worauf kommt es beim Management von Personen mit infektiöser Endokarditis an? Eine Kardiologin und ein Kardiologe fassen die zehn wichtigsten Punkte der neuen ESC-Leitlinie zusammen.

Update Innere Medizin

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert.