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01.07.2009 | Original Article

Phage-displayed antibody fragments recognizing dengue 3 and dengue 4 viruses as tools for viral serotyping in sera from infected individuals

verfasst von: Sheila Cabezas, Gertrudis Rojas, Alequis Pavon, Lidice Bernardo, Yinet Castellanos, Mayling Alvarez, Maritza Pupo, Gerardo Guillen, Maria G. Guzman

Erschienen in: Archives of Virology | Ausgabe 7/2009

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Abstract

The current study shows the usefulness of dengue-3- and dengue-4-specific phage-displayed antibody fragments as tools for viral detection and serotyping in sera from infected individuals. C6/36 HT cells were inoculated with acute-phase sera from patients, and supernatants were collected daily and analyzed by ELISA using phage-displayed antibody fragments as serotype-specific detector reagents. Serotyping of most samples was possible as early as two to three days postinoculation. Results were comparable with those obtained by indirect immunofluorescence assay but were obtained in a shorter period of time (<1 week). Phage-displayed antibody fragments were better tools for diagnosis and serotyping than their soluble counterparts. Our approach combines the advantages of viral isolation and ELISA techniques. These results could be the basis for the development of a high-throughput method for identifying dengue virus serotypes, which is crucial for the management and control of the disease.

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Titel: Phage-displayed antibody fragments recognizing dengue 3 and dengue 4 viruses as tools for viral serotyping in sera from infected individuals
verfasst von: Sheila Cabezas
Gertrudis Rojas
Alequis Pavon
Lidice Bernardo
Yinet Castellanos
Mayling Alvarez
Maritza Pupo
Gerardo Guillen
Maria G. Guzman
Publikationsdatum: 01.07.2009
Verlag: Springer Vienna
Erschienen in: Archives of Virology / Ausgabe 7/2009
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-009-0401-1

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