nach oben

European Spine Journal

Erschienen in:

23.02.2016 | Original Article

Plasmacytoid dendritic cells and memory T cells infiltrate true sequestrations stronger than subligamentous sequestrations: evidence from flow cytometric analysis of disc infiltrates

verfasst von: Andrea Geiss, Rolf Sobottke, Karl Stefan Delank, Peer Eysel

Erschienen in: European Spine Journal | Ausgabe 5/2016

Einloggen, um Zugang zu erhalten

Abstract

Purpose

Herniated nucleus pulposus has been considered to induce an adaptive immune response. Antigen recognition by antigen-presenting-cells (APCs) represents an important step within manifestation of an adaptive immune response. Macrophages have been assumed to function as APC, while importance of plasmacytoid dendritic cells for initiation of an immune response directed towards herniated nucleus pulposus has never been examined. The aim of the present study was to assess importance of plasmacytoid dendritic cells for initiation of immune response directed towards herniated discs.

Methods

Fifteen patients with true sequestrations and three patients with subligamentous sequestrations underwent surgery after their neurological examinations. Disc material was harvested, weighted and digested for 90 min. Separated single cells were counted, stained for plasmacytoid dendritic cells (CD123⁺CD4⁺), macrophages (CD14⁺CD11c⁺) and memory T cells (CD4⁺CD45RO⁺) and analysed by flow cytometry. Both patient groups were compared in cell proportions. Furthermore, patients with true sequestrations (TRUE patients) were subdivided into subgroups based on severity of muscle weakness and results in straight leg raising (SLR) test. Subgroups were compared in cell proportions.

Results

Plasmacytoid dendritic cells and memory T cells infiltrated true sequestrations stronger than the subligamentous sequestration and plasmacytoid dendritic cells predominated over macrophages in true sequestrations. Highest proportions of plasmacytoid dendritic cells were detected in infiltrates of patients having true sequestrations, severe muscle weakness and negative result in SLR test.

Conclusions

The findings of the present study indicate that plasmacytoid dendritic cells are involved in initiation of an immune response directed towards herniated nucleus pulposus, while macrophages may reinforce the manifested immune response and mediate disc resorption.

Mixter WJ, Barr J (1934) Rupture of the intervertebral disc with involvement of the spinal canal. N Engl J Med 211:210–215CrossRef

Rydevik B, Brown MD, Lundborg G (1984) Pathoanatomy and pathophysiology of nerve root compression. Spine 9:7–15CrossRefPubMed

Garfin SR, Rydevik BL, Brown RA (1991) Compressive neuropathy of spinal nerve roots. A mechanical or biological problem? Spine 16:162–166PubMed

Olmarker K, Larsson K (1998) Tumor necrosis factorα and nucleus pulposus-induced nerve root injury. Spine 23:2538–2544CrossRefPubMed

Geiss A, Larsson K, Junevik K et al (2009) Autologous nucleus pulposus primes T cells to develop into interleukin-4-producing effector cells: an experimental study on the autoimmune properties of nucleus pulposus. J Orthop Res 27:97–103CrossRefPubMed

Bobechko WP, Hirsch C (1965) Auto-immune response to nucleus pulposus in the rabbit. J Bone Joint Surg 47:574–580

Gertzbein SD, Trait JH, Devlin SR (1977) The stimulation of lymphocytes by nucleus pulposus in patients with degenerative disc disease of the lumbar spine. Clin Orthop Relat Res 123:149–154PubMed

Ahn SH, Ahn MW, Byun WM (2000) Effect of the Transligamentous extension of lumbar disc herniation on their regression and the clinical outcome of sciatica. Spine 25:475–480CrossRefPubMed

Brock M, Patt S, Mayer HM (1992) The form and structure of the extruded disc. Spine 17:1457–1461CrossRefPubMed

10.

Abbas AK, Lichtman AH, Pillai S (2012) Cellular and molecular immunology. Saunders/Elsevier, Philadelphia

11.

Saal JS, Franson RC, Dobrow R et al (1990) High levels of inflammatory phospholipase A₂ activity in lumbar disc herniations. Spine 15:674–678CrossRefPubMed

12.

Kobayashi S, Yoshizawa H, Yamada S (2004) Pathology of lumbar nerve root compression Part 1: intraradicular inflammatory changes induced by mechanical compression. J Orthop Res 22:170–179CrossRefPubMed

13.

Cui JG, Holmin S, Mathiesen T et al (2000) Possible role of inflammatory mediators in tactile hypersensitivity in rat models of mononeuropathy. Pain 88:239–248CrossRefPubMed

14.

Boos N, Rieder R, Schade V et al (1995) The diagnostic accuracy of magnetic resonance imaging, work perception, and psychosocial factors in identifying symptomatic disc herniations. Spine 20:2613–2625CrossRefPubMed

15.

Virri J, Grönblad M, Seitsalo S et al (2001) Comparison of the prevalence of inflammatory cells in subtypes of disc herniations and associations with straight leg raising. Spine 26:2311–2315CrossRefPubMed

16.

Grönblad M, Virri J, Seitsalo S et al (2000) Inflammatory cells, motor weakness, and straight leg raising in transligamentous disc herniations. Spine 25:2803–2807CrossRefPubMed

17.

Shamji MF, Setton LA, Jarvis W et al (2010) Proinflammatory cytokine expression profile in degenerated and herniated human intervertebral disc tissues. Arthritis Rheum 62:1974–1982PubMedPubMedCentral

18.

Rothoerl R, Woertgen C, Holzschuh M et al (1998) Macrophage tissue infiltration, clinical symptoms, and signs in patients with lumbar disc herniation. A clinicopathological study on 179 patients. Acta Neurochir 140:1245–1248CrossRefPubMed

19.

Kawaguchi S, Yamashita T, Yokogushi K et al (2001) Immunophenotypic analysis of the inflammatory infiltrates in herniated intervertebral discs. Spine 26:1209–1214CrossRefPubMed

20.

Colonna M, Trinchieri G, Liu YJ (2004) Plasmacytoid dendritic cells in immunity. Nat Immunol 5:1219–1226CrossRefPubMed

21.

Andersson GB, Weinstein JN (1996) Disc herniation. Spine 21(Suppl 24):1S

22.

Masaryk TJ, Ross JS, Modic MT et al (1988) High-resolution MR imaging of sequestered lumbar inter-vertebral disks. Am J Roentgenol 150:1155–1162CrossRef

23.

Frankel HL, Hancock DO, Hyslop G et al (1969) The value of postural reduction in the initial management of closed injuries of the spine with paraplegia and tetraplegia. I Paraplegia 7:179–192CrossRefPubMed

24.

Balaji VR, Chin KF, Tucker S (2014) Recovery of severe motor deficit secondary to herniated lumbar disc prolapse: is surgical intervention important? A systematic review. Eur Spine J 23:1968–1977CrossRefPubMed

25.

Hornsby PJ (1980) Regulation of cytochrome P-450 supported 11-hydroxylation of deoxycortisol by steroids, oxygen, and antioxidants in adrenocortical cell cultures. J Biol Chem 255:4020–4027PubMed

26.

Facchetti F, Vermi W, Mason D, Colonna M (2003) The plasmacytoid monocytes/interferon producing cells. Virchows Arch 443:703–717CrossRefPubMed

27.

Bland M (2000) An introduction to medical statistics, 3rd edn. Oxford University Press, Oxford

28.

Risbud MV, Shapiro IM (2014) Role of cytokines in intervertebral disc degeneration: pain and disc content. Nat Rev Rheumatol 10:44–56CrossRefPubMedPubMedCentral

29.

Buckwalter JA (1995) Aging and degeneration of the human intervertebral disc. Spine 20:1307–1314CrossRefPubMed

30.

Holm S, Nachemson A (1988) Nutrition of the intervertebral disc: acute effects of cigarette smoking. An experimental animal study. Upsala J Med 93:91–99CrossRef

31.

Murray PJ, Wynn TA (2011) Protective and pathogenic functions of macrophage subsets. Nat Rev Immunol 11:723–737CrossRefPubMedPubMedCentral

32.

Kapsenberg ML (2003) Dendritic-cell control of pathogen-driven T-cell polarization. Nat Rev Immunol 3:984–993CrossRefPubMed

33.

Liu YJ (2005) IPC: professional type I interferon-producing cells and plasmacytoid dendritic cell precursors. Annu Rev Immunol 23:275–306CrossRefPubMed

34.

Swiecki M, Colonna M (2015) The multifaceted biology of plasmacytoid dendritic cells. Nat Rev Immunol 15:471–485CrossRefPubMedPubMedCentral

35.

Gilliet M, Cao W, Liu YJ (2008) Plasmacytoid dendritic cells: sensing nucleic acids in viral infection and autoimmune diseases. Nat Rev Immunol 8:594–606CrossRefPubMed

36.

Lövgren T, Eloranta M-L, Bave U, Alm GV, Rönnblom L (2004) Induction of Interferon- production in plasmacytoid dendritic cells by immune complexes containing nucleic acid released by necrotic or late apoptotic cells and lupus IgG. Arthritis Rheum 50:1861–1872CrossRefPubMed

37.

Villadangos JA, Young L (2008) Antigen-presentation properties of plasmacytoid dendritic cells. Immunity 29:352–361CrossRefPubMed

38.

Stirling A, Worthington T, Rafiq M, Lambert PA, Elliot TSJ (2001) Association between sciatica and Propionibacterium acnes. Lancet 357:2014–2025CrossRef

39.

Gank R, Rao PJ, Phan K, Mobbs RJ (2015) Can bacterial infection by low virulent organisms be a plausible cause for symptomatic disc degeneration? Spine 40:E587–E592CrossRef

40.

Lotz JC, Chin JR (2000) Intervertebral disc cell death is dependent on the magnitude and duration of spinal loading. Spine 25:1477–1483CrossRefPubMed

41.

Bibby SRS, Urban JPG (2004) Effect of nutrient deprivation on the viability of intervertebral disc cells. Eur Spine J 13:695–701CrossRefPubMedPubMedCentral

42.

Satoh K, Konno S, Nishiyama K et al (1999) Presence and distribution of antigen-antibody complexes in the herniated nucleus pulposus. Spine 24:1980–1984CrossRefPubMed

43.

Klawitter M, Hakozaki M, Kobayashi H et al (2014) Expression and regulation of toll-like receptors (TLRs) in human intervertebral disc cells. Eur Spine J 23:1878–1891CrossRefPubMed

44.

Cella M, Jarrossay D, Facchetti F et al (1999) Plasmacytoid monocytes migrate to inflamed lymph nodes and produce large amounts of type I interferon. Nature Med 5:919–923CrossRefPubMed

45.

Bondue B, Wittamer V, Parmentier M (2011) Chemerin and its receptors in leukocyte trafficking, inflammation and metabolism. Cytokine Growth Factor Rev 22:331–338CrossRefPubMed

46.

Doita M, Kanatani T, Ozaki T, Matsui N, Kurosaka M, Yoshiya S (2001) Influence of macrophage infiltration of herniated disc tissue on the production of matrix metalloproteinases leading to disc resorption. Spine 26:1522–1527CrossRefPubMed

47.

Ikeda T, Nakamura T, Kikuchi T, Umeda S, Senda H, Takagi K (1996) Pathomechanism of spontaneous regression of the herniated lumbar disc: histologic and immunohistochemical study. J Spinal Disord 9:136–140CrossRefPubMed

48.

Campbell DJ, Koch MA (2011) Phenotypical and functional specialization of FOXP3⁺regulatory T cells. Nat Rev Immunol 11:119–130CrossRefPubMedPubMedCentral

49.

Rissoan MC, Soumelis V, Kadowaki N et al (1999) Reciprocal control of T helper cell and dendritic cell differentiation. Science 283:1183–1186CrossRefPubMed

50.

Agak GW, Qin M, Nobe J et al (2014) Propionibacterium acnes induces an IL-17 response in acne vulgaris that is regulated by vitamin A and vitamin D. J Invest Dermatol 134:366–373CrossRefPubMedPubMedCentral

51.

Shin MS, Lee N, Kang I (2011) Effector T cell subsets in systemic lupus erythematosus: update focusing in TH17 cells. Curr Opin Rheumatol 23:444–448CrossRefPubMedPubMedCentral

52.

Park JB, Chang H, Kim YS (2002) The pattern of interleukin-12 and T-helper types 1 and 2 cytokine expression in herniated lumbar disc tissue. Spine 27:2125–2128CrossRefPubMed

53.

Gilfillan AM, Tkaczyk C (2006) Integrated signalling pathways for mast-cell activation. Nat Rev Immunol 6:218–230CrossRefPubMed

54.

Garcia-Faroldi G, Rönnberg E, Orro A et al (2013) ADAMTS: novel proteases expressed by activated mast cells. Biol Chem 394:291–305CrossRefPubMed

55.

Geiss A, Larsson K, Rydevik B et al (2007) Autoimmune properties of nucleus pulposus.An experimental study in pigs. Spine 32:168–173CrossRefPubMed

56.

Richards JO, Treisman J, Garlie N et al (2012) Flow cytometry assessment of residual melanoma cells in tumor-infiltrating lymphocytes cultures. Cytometry A 81:374–381CrossRefPubMed

57.

Waymouth C (1974) To disaggregate or not to disaggregate injury and cell disaggregation, transient or permanent? In Vitro 10:97–111CrossRefPubMed

58.

Abuzakouk M, Feighery C, O’Farrelly C (1996) Collagenase and Dispase enzymes disrupt lymphocyte surface molecules. J Immunol Methods 194:211–216CrossRefPubMed

59.

Lasfargues EY, Moore DH (1971) A method for the continuous cultivation of mammary epithelium. In Vitro 7:21–25CrossRefPubMed

60.

Delaney TJ, Rowlingson JC, Carron H, Butler A (1980) Epidural steroid effects on nerves and meninges. Anesth Analg 59:610–614PubMed

61.

Olmarker K, Byröd G, Cornefjord M et al (1994) Effects of Methylprednisolone on nucleus pulposus-induced nerve root injury. Spine 19:1803–1808CrossRefPubMed

Titel: Plasmacytoid dendritic cells and memory T cells infiltrate true sequestrations stronger than subligamentous sequestrations: evidence from flow cytometric analysis of disc infiltrates
verfasst von: Andrea Geiss
Rolf Sobottke
Karl Stefan Delank
Peer Eysel
Publikationsdatum: 23.02.2016
Verlag: Springer Berlin Heidelberg
Erschienen in: European Spine Journal / Ausgabe 5/2016
Print ISSN: 0940-6719
Elektronische ISSN: 1432-0932
DOI: https://doi.org/10.1007/s00586-015-4325-z

Arthropedia

Grundlagenwissen der Arthroskopie und Gelenkchirurgie. Erweitert durch Fallbeispiele, Videos und Abbildungen.
» Jetzt entdecken

Neu im Fachgebiet Orthopädie und Unfallchirurgie

25.04.2024 | Hypertonie | Nachrichten

Update Orthopädie und Unfallchirurgie

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert.

Newsletter bestellen

Springer Medizin

Plasmacytoid dendritic cells and memory T cells infiltrate true sequestrations stronger than subligamentous sequestrations: evidence from flow cytometric analysis of disc infiltrates

Abstract

Purpose

Methods

Results

Conclusions

Arthropedia

Neu im Fachgebiet Orthopädie und Unfallchirurgie

Therapiestart mit Blutdrucksenkern erhöht Frakturrisiko

Ärztliche Empathie hilft gegen Rückenschmerzen

Stereotaktische Radiatio schlägt konventionelle Bestrahlung

Vorsicht mit hohen NSAR-Dosen bei ankylosierender Spondylitis!

Update Orthopädie und Unfallchirurgie

Springer Medizin

Abstract

Purpose

Methods

Results

Conclusions

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 5/2016

A comparative study of perioperative complications between transforaminal versus posterior lumbar interbody fusion in degenerative lumbar spondylolisthesis

Three-dimensional analysis of volumetric changes in herniated discs of the lumbar spine: does spontaneous resorption of herniated discs always occur?

Adjacent segment degeneration and revision surgery after circumferential lumbar fusion: outcomes throughout 15 years of follow-up

Are lumbar multifidus fatigue and transversus abdominis activation similar in patients with lumbar disc herniation and healthy controls? A case control study

Risk factors for the need of surgical treatment of a first recurrent lumbar disc herniation

Does usage of a parachute in contrast to free fall prevent major trauma?: a prospective randomised-controlled trial in rag dolls

Arthropedia

Neu im Fachgebiet Orthopädie und Unfallchirurgie

Therapiestart mit Blutdrucksenkern erhöht Frakturrisiko

Ärztliche Empathie hilft gegen Rückenschmerzen

Stereotaktische Radiatio schlägt konventionelle Bestrahlung

Vorsicht mit hohen NSAR-Dosen bei ankylosierender Spondylitis!

Update Orthopädie und Unfallchirurgie