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Osteoporosis International

Erschienen in:

01.02.2014 | Original Article

Prednisolone induces osteoporosis-like phenotype in regenerating zebrafish scales

verfasst von: E. de Vrieze, M. A. H. J. van Kessel, H. M. Peters, F. A. T. Spanings, G. Flik, J. R. Metz

Erschienen in: Osteoporosis International | Ausgabe 2/2014

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Abstract

Summary

We demonstrate that glucocorticoids induce an osteoporotic phenotype in regenerating scales of zebrafish. Exposure to prednisolone results in altered mineral content, enhanced matrix breakdown, and an osteoporotic gene-expression profile in osteoblasts and osteoclasts. This highlights that the zebrafish scale provides a powerful tool for preclinical osteoporosis research.

Introduction

This study aims to evaluate whether glucocorticoid (prednisolone) treatment of zebrafish induces an osteoporotic phenotype in regenerating scales. Scales, a readily accessible dermal bone tissue, may provide a tool to study direct osteogenesis and its disturbance by glucocorticoids.

Methods

In adult zebrafish, treated with 25 μM prednisolone phosphate via the water, scales were removed and allowed to regenerate. During regeneration scale morphology and the molar calcium/phosphorus ratio in scales were assessed and osteoblast and osteoclast activities were monitored by time profiling of cell-specific genes; mineralization was visualized by Von Kossa staining, osteoclast activity by tartrate-resistant acid phosphatase histochemistry.

Results

Prednisolone (compared to controls) enhances osteoclast activity and matrix resorption and slows down the build up of the calcium/phosphorus molar ratio indicative of altered crystal maturation. Prednisolone treatment further impedes regeneration through a shift in the time profiles of osteoblast and osteoclast genes that commensurates with an osteoporosis-like imbalance in bone formation.

Conclusions

A glucocorticoid-induced osteoporosis phenotype as seen in mammals was induced in regenerating scalar bone of zebrafish treated with prednisolone. An unsurpassed convenience and low cost then make the zebrafish scale a superior model for preclinical studies in osteoporosis research.

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Titel: Prednisolone induces osteoporosis-like phenotype in regenerating zebrafish scales
verfasst von: E. de Vrieze
M. A. H. J. van Kessel
H. M. Peters
F. A. T. Spanings
G. Flik
J. R. Metz
Publikationsdatum: 01.02.2014
Verlag: Springer London
Erschienen in: Osteoporosis International / Ausgabe 2/2014
Print ISSN: 0937-941X
Elektronische ISSN: 1433-2965
DOI: https://doi.org/10.1007/s00198-013-2441-3

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Abstract

Summary

Introduction

Methods

Results

Conclusions

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