Background
Enterococci are normal inhabitants of the gastrointestinal tract of humans and animals. Two species cause most enterococcal infections,
Enterococcus faecalis and
E. faecium. Enterococci cause urinary tract infections mainly followed by intra-abdominal and pelvic infections. They also cause surgical wound infections, bacteraemia, endocarditis, neonatal sepsis and rarely meningitis [
1].
Among the risk factors, previous antibiotic treatment [
2], duration of hospitalization (≥7 days), and duration of vancomycin use (≥7 days) [
3], surgical units or intensive-care units; co-morbidities such as diabetes, renal failure; and the presence of a urinary catheter [
4], are among the many risk factors for colonization or infection with vancomycin resistance
Enterococci (VRE). Critically ill patients or those with severe underlying disease or immunosuppression such as persons who have had an intra abdominal or cardio-thoracic surgical procedure or an indwelling urinary or central venous catheter are also important risk factors [
5]. Moreover, HIV-positive patients often receive antibiotic therapy and have frequent contact with the healthcare system, both of which are factors that have been associated with an increased risk of infection with VRE in other populations [
6].
The relative importance of
Enterococcus as a pathogen has increased with the occurrence of high-level resistance to multiple antimicrobial drugs, such as ampicillin and vancomycin [
7]. The emergence of VRE has alarmed the global infectious diseases community due to few option left for disease management, resistance gene transfer from
Enterococci to
Staphylococcus aureus and presence of different selection pressures for VRE proliferation and rapid expansion of resistant populations [
8]. The prevalence of VRE was reported in Europe, Asia, Australia, South America and some countries of Africa [
9]. However, there is no enough data available on the epidemiology and risk factors of VRE in Ethiopia. The present study aimed to assess the prevalence of VRE, their susceptibility patterns to different antibiotics and associated risk factors in fecal samples of HIV positive and HIV negative clients at the University of Gondar Teaching Hospital.
Methods
Study design, area and period
A cross sectional study was carried out from July 1/2013 to September 30/2013 at the University of Gondar Teaching Hospital. The hospital is located in Gondar town, 737 km Northwest of Addis Ababa. It has 400 beds and provides health care referral services for over 5 million inhabitants in Northwest Ethiopia. The University Teaching hospital has ART clinic and blood bank center.
Study population
HIV positive patients attending ART clinic and HIV negative blood donors at the University of Gondar Teaching Hospital during the study period were enrolled in this study.
Sample size and sampling technique
The sample size was determined by using EPI INFO version 3.5.3 in double population proportion. Since there is no previous study done in this area, 50% prevalence was used for HIV negative clients. HIV positive patients are assumed to have 20% more VRE carriage than HIV negative clients, 70%. Considering the two prevalence, power 80% at 95% confidence with 1:1 ratio the final sample size was 226 (113 HIV infected patients and 113 HIV negative blood donors). The last 3 years hospital report showed that an average of 57 HIV positive clients was visited the ART laboratory daily. We calculated the total participants in 3 months study period (57 × 90 days = 5130). Since our sample size is 113 HIV positive patients we calculated the k = value to be 45. The first one was selected with lottery method from 1-45 cases. Then every 45th patients were included in this study. Consecutive HIV negative volunteers blood donors (n = 113) were also included in the study.
Data collection
Socio-demographic and clinical data and other possible factors responsible for colonization of VRE such as antimicrobial therapy, drinking alcohol and others were collected using pre-tested structured questionnaire. Five milliliters of blood was drawn from each client by an experienced laboratory technologist. The HIV status of the clients was determined using rapid test kit (KHB, STAT pak and Uni-Gold) following the current Ethiopian algorithm [
10]. The CD
4 count (for HIV positives) and complete blood count were done using BD FACS count and Sysmex Machine respectively.
The clients were provided with wide-mouthed, sterile plastic containers and informed to submit stool specimens. The collected stool specimens were streaked on Bile Esculin Azide agar (Hardy Diagnostics, Santa Maria, CA, USA) and incubated for 24 hours at 37°C. Plates were observed for appearance of characteristic colonies of growth and blackening. Typical characteristic colonies were selected randomly for characterization and presumptive identification of
Enterococcci[
11]. Each isolate was also assessed using Gram staining, catalase test and its growth in 6.5% NaCl broth. Presumptive pure colonies were picked and inoculated into Brain Heart Infusion (BHI) broth, and incubated at 45°C for 24 hours and growth in the medium was assessed by its turbidity. An isolate fulfilling the above criteria was assumed to be
Enterococccus species [
11].
Antimicrobial susceptibility testing
Antimicrobial susceptibility testing for each isolate was done using Muller-Hinton agar (OXOID, UK) disc diffusion method [
12]. Antimicrobial susceptibility patterns of
Enterococci were assessed against nine antibiotic discs (all OXOID, UK). These were vancomycin 30 μg, ampicillin 10 μg, amoxicillin 10 μg, amoxicillin-clavulinic acid 10 μg, ciprofloxacin 5 μg, chloramphenicol 30 μg, erythromycin 15 μg, gentamicin 10 μg and trimethoprim-sulfamethoxazole 1.25/23.75 μg.
Quality control
Reference strain of Staphylococcus aureus ATCC 25923 and E. faecalis ATCC 51299 were used as negative and positive control, respectively.
Data analysis
Data were entered and analyzed using SPSS version 20. Descriptive statistics were employed and odds ratio (OR) was used to determine the strength of association. Multivariate analysis using logistic regression model was used to analyze the association between VRE colonization and potential risk factors. Those variables with over all P value less than 0.2 in the bivariate analysis were entered into multivariate analysis. P-values < 0.05 was considered statistically significant.
Definitions: Leukocytosis and leucopenia were defined as having WBC count greater than 12,000/mm3 and less than 4,000/mm3, respectively. A client was named as thrombocytopenic when she/he had a platelet count of <100,000/ μL.
Ethical consideration
The study was approved by the research and ethics committee of the School of Biomedical and Laboratory Sciences, University of Gondar. Written informed consent was also obtained from each client.
Discussion
In the present study the overall prevalence of
Enterococci was 201/226(88.9%). This was consistent with the previous report from Israel 88.5% [
13]. However, it was lower than a report from Algeria (100%) [
14]. This variation in colonization might be due to the advanced molecular method in the previous study.
The prevalence of
Enterococci was higher in HIV positives (91.2%) than HIV negatives (86.7%) clients. However, this was not statistically significant (P = 0.14).This result was in line with reports by Hijazi [
15] in hospitalized patients (94%) and in individuals living in the community (89%).
The prevalence of VRE in this study was 11/201(5.5%). This was consistent with reports from Korea, 4.5% [
16] and lower than reports from Egypt (25%) isolated from pediatric patients [
17] and health care workers in intensive care units (9.5%) [
18]. These differences might be due to the study design and study participants (pediatric age and intensive care unit) in the previous study.
The prevalence of VRE among HIV positives (7.8%) in the present study was higher than reports from USA, 0% [
19] and 4.7% [
6]. This variation might be due to difference in the study population where most of the clients in the present study had habit of animal contact and this was supported by Bekele and Ashenafi, reported as 100% VRE
Enterococci isolated from faeces of chicken and cattle in Ethiopia [
20]. Comparison of colonization of VRE among HIV positives (7.8%) and HIV negative (3.1%) clients’ revealed that there was a slightly higher VRE in HIV positive patients. The higher VRE among HIV positives might be due to repeated exposure to different antibiotics [
6] and immune-suppression [
21] which are important risk factors for colonization or infection with VRE.
Among risk factors studied for VRE, a patient with a history of prior antibiotic use was found to be important factor (P = 0.01). Similar reports from Iran showed that antibiotic exposure can cause the emergence of VRE by inducing the expression of resistance genes and by selecting strains already expressing these genes and altering the competing microbial flora in the GI tract, thereby increasing VRE concentration in the stools [
22,
23]. However, stool consistency, CD4+ count, HARRT, HARRT duration (year),WBC and platelet count, nutritional status, anaemia, drinking alcohol, WHO stage for HIV were not statistically significant risk factors for VRE (P > 0.05). This was consistent with previous study in USA [
6].
Among 201 isolates of
Enterococci 181 (90%) were resistant to two or more antibiotics tested. Similarly over 80% of multiple drug resistance (MDR)
Enterococci isolates were reported from cattle in Addis Ababa [
20]. Although high percentage of resistance against ampicillin (79.6), gentamaycin (71.6) and erythromycin (63.2) were observed, no significant difference was seen between HIV positive and HIV negative clients. However, the resistance pattern of
Enterococcus isolates to amoxicillin and amoxicillin-clavulinic acid were significantly higher in HIV positive than HIV negative clients. This significant difference might be due to the frequent prescription of broad spectrum antibiotic for HIV patients. The high prevalence of MDR enterococccal colonization in humans may lead to infection with reduced treatment options.
Limitation of the study
The isolated enterococcci were not indentified to species level.
Conclusion
The prevalence of VRE in clients attending the University of Gondar Hospital was 5.5%. Patients with prior exposure to antibiotics for more than two weeks had higher colonization than their respective group. The presence of 11 (5.5%) prevalence of VRE in this study signals the emergence of VRE in the study area. Therefore, rational use of antibiotics and more detailed study using phenotypic and genotypic methods are needed.
Competing interests
The authors declare no conflict of interest exists.
Authors’ contributions
WA, ME and FM: conceived the idea, write the proposal, processes clinical samples, data analysis and draft the manuscript for publication. MT: assisted during sample processing. All the authors read and approved the manuscript.