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Erschienen in: International Urogynecology Journal 4/2012

01.04.2012 | Original Article

Role of estrogen receptor alpha on vaginal epithelialization of patients with Mayer–Rokitansky–Kuster–Hauser syndrome submitted to neovaginoplasty using oxidized regenerated cellulose

verfasst von: Marcio Masashi Kajikawa, Zsuzsanna Ilona Katalin Jármy-Di Bella, Gustavo Rubino de Azevedo Focchi, Juliane Dornelas, Manoel João Batista Castello Girão, Marair Gracio Ferreira Sartori

Erschienen in: International Urogynecology Journal | Ausgabe 4/2012

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Abstract

Introduction and hypothesis

The purpose of this study was to evaluate the expression of estrogen receptor alpha (ERα) on the neovaginal tissue of patients with Mayer–Rokitansky–Kuster–Hauser (MRKH) syndrome submitted to modified Abbé-McIndoe neovaginoplasty using oxidized regenerated cellulose.

Methods

The current study involved eight subjects with MRKH syndrome. Serial samples of the neovagina were obtained after the surgery and immunohistochemical detection of ERα was performed using antibody NCL-L-ER-6F11.

Results

The operation was performed successfully in all subjects without complications. A total of 22 samples of neovaginal tissue were analyzed. The expression of ERα was detected only 6 months after the surgery, when the neovagina acquired characteristics of normal vagina.

Conclusion

In our study, the expression of ERα occurred when complete epithelialization of vaginal tissue was observed. Other mechanisms may be involved in the formation of vaginal epithelium in patients with MRKH syndrome.
Literatur
1.
Zurück zum Zitat Morcel K, Camborieux L, Programme de Recherches sur les Aplasies Müllériennes (PRAM), Guerrier D (2007) Mayer-Rokitansky-Küster-Hauser (MRKH) syndrome. Orphanet J Rare Dis 2:13PubMedCrossRef Morcel K, Camborieux L, Programme de Recherches sur les Aplasies Müllériennes (PRAM), Guerrier D (2007) Mayer-Rokitansky-Küster-Hauser (MRKH) syndrome. Orphanet J Rare Dis 2:13PubMedCrossRef
2.
Zurück zum Zitat Laufer MR (2002) Congenital absence of the vagina: in search of the perfect solution. When, and by what technique, should a vagina be created? Curr Opin Obstet Gynecol 14:441–444PubMedCrossRef Laufer MR (2002) Congenital absence of the vagina: in search of the perfect solution. When, and by what technique, should a vagina be created? Curr Opin Obstet Gynecol 14:441–444PubMedCrossRef
3.
Zurück zum Zitat McIndoe AH, Bannister JB (1938) An operation for the cure of congenital absence of the vagina. J Obstet Gynaecol Br Emp 45:490–494CrossRef McIndoe AH, Bannister JB (1938) An operation for the cure of congenital absence of the vagina. J Obstet Gynaecol Br Emp 45:490–494CrossRef
4.
Zurück zum Zitat El-Sayed HM, El-Lamie IK, Ibrahim AM, El-Lamie KI (2007) Vaginal reconstruction with sigmoid colon in vaginal agenesis. Int Urogynecol J Pelvic Floor Dysfunct 18:1043–1047PubMedCrossRef El-Sayed HM, El-Lamie IK, Ibrahim AM, El-Lamie KI (2007) Vaginal reconstruction with sigmoid colon in vaginal agenesis. Int Urogynecol J Pelvic Floor Dysfunct 18:1043–1047PubMedCrossRef
5.
Zurück zum Zitat Ashworth MF, Morton KE, Dewhurst J, Lilford RJ, Bates RG (1986) Vaginoplasty using amnion. Obstet Gynecol 67:443–446PubMed Ashworth MF, Morton KE, Dewhurst J, Lilford RJ, Bates RG (1986) Vaginoplasty using amnion. Obstet Gynecol 67:443–446PubMed
6.
Zurück zum Zitat Marques HS, Santos FL, Costa PVL, Santos AR, Silva BB (2008) Creation of a neovagina in patients with Rokitansky syndrome using peritoneum from the pouch of Douglas: an analysis of 48 cases. Fertil Steril 90:827–832CrossRef Marques HS, Santos FL, Costa PVL, Santos AR, Silva BB (2008) Creation of a neovagina in patients with Rokitansky syndrome using peritoneum from the pouch of Douglas: an analysis of 48 cases. Fertil Steril 90:827–832CrossRef
7.
Zurück zum Zitat Jackson ND, Rosenblatt PL (1994) Use of interceed absorbable adhesion barrier for vaginoplasty. Obstet Gynecol 84:1048–1050PubMed Jackson ND, Rosenblatt PL (1994) Use of interceed absorbable adhesion barrier for vaginoplasty. Obstet Gynecol 84:1048–1050PubMed
8.
Zurück zum Zitat Motoyama S, Laoag-Fernandez JB, Mochizuki S, Yamabe S, Maruo T (2003) Vaginoplasty with interceed absorbable adhesion barrier for complete squamous epithelialization in vaginal agenesis. Am J Obstet Gynecol 188:1260–1264PubMedCrossRef Motoyama S, Laoag-Fernandez JB, Mochizuki S, Yamabe S, Maruo T (2003) Vaginoplasty with interceed absorbable adhesion barrier for complete squamous epithelialization in vaginal agenesis. Am J Obstet Gynecol 188:1260–1264PubMedCrossRef
9.
Zurück zum Zitat Ahmad G, Duffy JM, Farquhar C, Vail A, Vandekerckhove P, Watson A, Wiseman D (2008) Barrier agents for adhesion prevention after gynaecological surgery. Cochrane Database Syst Rev 16:CD000475 Ahmad G, Duffy JM, Farquhar C, Vail A, Vandekerckhove P, Watson A, Wiseman D (2008) Barrier agents for adhesion prevention after gynaecological surgery. Cochrane Database Syst Rev 16:CD000475
10.
Zurück zum Zitat Bekerecioglu M, Balat O, Tercan M, Karakok M, Ugur MG, Isik D (2008) Adaptation process of the skin graft to vaginal mucosa after McIndoe vaginoplasty. Arch Gynecol Obstet 277:551–554PubMedCrossRef Bekerecioglu M, Balat O, Tercan M, Karakok M, Ugur MG, Isik D (2008) Adaptation process of the skin graft to vaginal mucosa after McIndoe vaginoplasty. Arch Gynecol Obstet 277:551–554PubMedCrossRef
11.
Zurück zum Zitat Bleggi-Torres LF, Werner B, Piazza MJ (1997) Ultrastructural study of the neovagina following the utilization of human amniotic membrane for treatment of congenital absence of the vagina. Braz J Med Biol Res 30:861–864PubMedCrossRef Bleggi-Torres LF, Werner B, Piazza MJ (1997) Ultrastructural study of the neovagina following the utilization of human amniotic membrane for treatment of congenital absence of the vagina. Braz J Med Biol Res 30:861–864PubMedCrossRef
12.
Zurück zum Zitat Sturdee DW, Panay N, International Menopause Society Writing Group (2010) Recommendations for the management of postmenopausal vaginal atrophy. Climacteric 13:509–522PubMedCrossRef Sturdee DW, Panay N, International Menopause Society Writing Group (2010) Recommendations for the management of postmenopausal vaginal atrophy. Climacteric 13:509–522PubMedCrossRef
13.
Zurück zum Zitat Archer DF (2010) Efficacy and tolerability of local estrogen therapy for urogenital atrophy. Menopause 17:194–203PubMedCrossRef Archer DF (2010) Efficacy and tolerability of local estrogen therapy for urogenital atrophy. Menopause 17:194–203PubMedCrossRef
14.
Zurück zum Zitat Pelletier G, El-Alfy M (2009) Immunocytochemical localization of estrogen receptors α and β in the human reproductive organs. J Clin Endocrinol Metab 85:4835–4839CrossRef Pelletier G, El-Alfy M (2009) Immunocytochemical localization of estrogen receptors α and β in the human reproductive organs. J Clin Endocrinol Metab 85:4835–4839CrossRef
15.
Zurück zum Zitat Pessina MA, Hoyt RF Jr, Goldstein I, Traish AM (2006) Differential regulation of the expression of estrogen, progesterone, and androgen receptors by sex steroid hormones in the vagina: immunohistochemical studies. J Sex Med 3:804–814PubMedCrossRef Pessina MA, Hoyt RF Jr, Goldstein I, Traish AM (2006) Differential regulation of the expression of estrogen, progesterone, and androgen receptors by sex steroid hormones in the vagina: immunohistochemical studies. J Sex Med 3:804–814PubMedCrossRef
17.
Zurück zum Zitat Sharma JB, Gupta N, Mittal S (2007) Creation of neovagina using oxidized celulose (surgicel) as a surgical treatment of vaginal agenesis. Arch Gynecol Obstet 275:231–235PubMedCrossRef Sharma JB, Gupta N, Mittal S (2007) Creation of neovagina using oxidized celulose (surgicel) as a surgical treatment of vaginal agenesis. Arch Gynecol Obstet 275:231–235PubMedCrossRef
18.
Zurück zum Zitat Cooke PS, Buchanan DL, Lubahn DB, Cunha GR (1998) Mechanism of estrogen action: lessons from the estrogen receptor-alpha knockout mouse. Biol Reprod 59:470–475PubMedCrossRef Cooke PS, Buchanan DL, Lubahn DB, Cunha GR (1998) Mechanism of estrogen action: lessons from the estrogen receptor-alpha knockout mouse. Biol Reprod 59:470–475PubMedCrossRef
19.
Zurück zum Zitat Dupont S, Krust A, Gansmuller A, Dierich A, Chambon P, Mark M (2000) Effect of single and compound knockouts of estrogen receptors α (REα) and β (REβ) on mouse reproductive phenotypes. Development 127:4277–4291PubMed Dupont S, Krust A, Gansmuller A, Dierich A, Chambon P, Mark M (2000) Effect of single and compound knockouts of estrogen receptors α (REα) and β (REβ) on mouse reproductive phenotypes. Development 127:4277–4291PubMed
20.
Zurück zum Zitat Buchanan D, Kurita T, Taylor JA, Lubahn DB, Cunha GR, Cooke PS (1998) Role of stromal and epithelial estrogen receptors in vaginal epithelial proliferation, stratification, and cornification. Endocrinology 139:4345–4352PubMedCrossRef Buchanan D, Kurita T, Taylor JA, Lubahn DB, Cunha GR, Cooke PS (1998) Role of stromal and epithelial estrogen receptors in vaginal epithelial proliferation, stratification, and cornification. Endocrinology 139:4345–4352PubMedCrossRef
21.
Zurück zum Zitat Smith EP, Boyd J, Frank GR et al (1994) Estrogen resistance caused by a mutation in the estrogen-receptor gene in a man. N Engl J Med 331:1056–1061PubMedCrossRef Smith EP, Boyd J, Frank GR et al (1994) Estrogen resistance caused by a mutation in the estrogen-receptor gene in a man. N Engl J Med 331:1056–1061PubMedCrossRef
22.
Zurück zum Zitat Jones ME, Boon WC, McInnes K, Maffei L, Carani C, Simpson ER (2007) Recognizing rare disorders: aromatase deficiency. Nat Clin Pract Endocrinol Metab 3:414–421PubMedCrossRef Jones ME, Boon WC, McInnes K, Maffei L, Carani C, Simpson ER (2007) Recognizing rare disorders: aromatase deficiency. Nat Clin Pract Endocrinol Metab 3:414–421PubMedCrossRef
23.
Zurück zum Zitat Ildgruben A, Sjöberg I, Hammarström ML, Bäckström T (2005) Steroid receptor expression in vaginal epithelium of healthy fertile women and influences of hormonal contraceptive usage. Contraception 72:383–392PubMedCrossRef Ildgruben A, Sjöberg I, Hammarström ML, Bäckström T (2005) Steroid receptor expression in vaginal epithelium of healthy fertile women and influences of hormonal contraceptive usage. Contraception 72:383–392PubMedCrossRef
24.
Zurück zum Zitat De Filippo RE, Bishop CE, Filho LF, Yoo JJ, Atala A (2008) Tissue engineering a complete vaginal replacement from a small biopsy of autologous tissue. Transplantation 86:208–214PubMed De Filippo RE, Bishop CE, Filho LF, Yoo JJ, Atala A (2008) Tissue engineering a complete vaginal replacement from a small biopsy of autologous tissue. Transplantation 86:208–214PubMed
25.
Zurück zum Zitat Panici PB, Bellati F, Boni T, Francescangeli F, Frati L, Marchese C (2007) Vaginoplasty using autologous in vitro cultured vaginal tissue in a patient with Mayer–von–Rokitansky–Kuster–Hauser syndrome. Hum Reprod 22:2025–2028PubMedCrossRef Panici PB, Bellati F, Boni T, Francescangeli F, Frati L, Marchese C (2007) Vaginoplasty using autologous in vitro cultured vaginal tissue in a patient with Mayer–von–Rokitansky–Kuster–Hauser syndrome. Hum Reprod 22:2025–2028PubMedCrossRef
Metadaten
Titel
Role of estrogen receptor alpha on vaginal epithelialization of patients with Mayer–Rokitansky–Kuster–Hauser syndrome submitted to neovaginoplasty using oxidized regenerated cellulose
verfasst von
Marcio Masashi Kajikawa
Zsuzsanna Ilona Katalin Jármy-Di Bella
Gustavo Rubino de Azevedo Focchi
Juliane Dornelas
Manoel João Batista Castello Girão
Marair Gracio Ferreira Sartori
Publikationsdatum
01.04.2012
Verlag
Springer-Verlag
Erschienen in
International Urogynecology Journal / Ausgabe 4/2012
Print ISSN: 0937-3462
Elektronische ISSN: 1433-3023
DOI
https://doi.org/10.1007/s00192-011-1604-z

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