Fig. 1
Vascularisation and survival of pancreatic islets transplanted under the kidney capsule of immune deficient NSG mice. (
a–
d) Maximum projection image (projecting all
xy focal planes into a single 2D image) of murine MIP-EGFP/CAG-DsRed islets transplanted under the kidney capsule. The grafts were imaged on day 1, 4, 8 and 15. MIP-EGFP (green), CAG-DsRed (red). Scale bar, 250 μm. (
e) Tissue volume of all pancreatic cells (CAG-DsRed, in red) and beta cells (MIP-EGFP, in green) in transplanted MIP-EGFP/CAG-DsRed islets over time. DsRed volume was significantly increased on day 15 compared with day 1. (
f) Percentage of the volume of beta cells (EGFP %) over whole tissue volume (DsRed) in transplanted MIP-EGFP/CAG-DsRed islets over time. The percentage of beta cells was significantly decreased on day 8 and 15 compared to day 1. (
g–
j) Human pancreatic islets were dispersed into single cells, transduced with a lentivirus containing a HIP-GFP virus, and reaggregated overnight on ultra-low attachment plates. After 1 week in culture, islet cell aggregates were visually assessed and transplanted if at least 50% of the cells were fluorescent. Maximum projection images of transplanted islets were captured on day 1, 4, 8 and 15. HIP-GFP (green). Scale bar, 250 μm. (
k) Image of a single
xy focal plane of transplanted MIP-EGFP islets 3 days after transplantation. Blood vessels (red) were visualised after a tail vein injection of Texas Red-conjugated dextran solution. The arrowhead marks a transplanted islet of which several
xy focal planes are merged into a mosaic in ESM Fig.
4. Scale bar, 100 μm. Data are mean ± SEM. *
p < 0.05, **
p < 0.01 vs day 1. TX, transplant