Introduction
Results
Identification of the overlapping DEGs
Enrichment analysis of the overlapping DEGs
Construction of the PPI network and selection of hub genes
MCC | MNC | Degree | The overlapping genes |
---|---|---|---|
SNAP25 | SNAP25 | SNAP25 | SNAP25 SYN1 SYT1 GAP43 SNAP91 |
SYN1 | SYN1 | SYT1 | |
SYT1 | SYT1 | SYN1 | |
SNAP91 | GAP43 | GAP43 | |
STXBP1 | SNAP91 | GABRG2 | |
DNM1 | NRXN1 | STMN2 | |
SYNJ1 | SNCB | SNCB | |
GAP43 | STMN2 | NRXN1 | |
AMPH | GRIA1 | SNAP91 | |
DNM3 | NEFL | NEFL |
Validation of representative hub genes by real-time qPCR in 6-month AD and PD mice
Olfactory deficit in the 6-month AD and PD mice
Correlation analysis of the SNAP25 mRNA between the OB and OE samples and the HIP or SN samples
Virtual screening of potential compounds targeting SNAP25
Drug name | Molecular formula | Docking Score (kcal/mol) | Noncovalent interactions | Residues |
---|---|---|---|---|
Pazopanib | C21H23N7O2S | − 9.2 | 6 Hydrophobic interactions, 7 H-bonds | ALA18C, HIS139D, GLU143D, ILE195B, MET14C, GLN15C, ALA18C, LYS189B, ALA191B, LEU192B, ARG198B |
S-apomorphine | C17H17NO2 | − 9.1 | 8 Hydrophobic interactions, 1 H-bond, 1 salt bridge | ALA22C, ARG142D, GLU143D, ILE195B, ARG198B, SER25C, GLU143D |
Paliperidone | C23H27FN4O3 | − 8.9 | 5 hydrophobic interactions, 1 H-bond | GLN15C, GLU143D, ILE195B, ARG198B, HIS139D |
Cromolyn | C23H16O11 | − 8.9 | 6 Hydrophobic interactions, 4 H-bonds, 1 salt bridge | ALA18C, ASP19C, GLU143D, ALA191B, ILE195B, GLN15C, LEU21C, ARG142D, HIS199B |
Risperdal | C23H27FN4O2 | − 8.8 | 5 Hydrophobic interactions, 1 H-bond | ALA18C, GLU143D, ALA191B, ILE195B, ARG198B, HIS139D |
Troglitazone | C24H27NO5S | − 8.8 | 7 Hydrophobic interactions, 1 H-bond | ALA18C, ALA22C, HIS139D, GLU143D, ALA191B, LEU192B, ILE195B, ARG198B |
Fanapt | C24H27FN2O4 | − 8.8 | 6 Hydrophobic interactions, 3 H-bonds | ALA18C, ALA22C, GLU143D, ILE195B, LYS189B, ALA191B, LEU192B |
Cinacalcet | C22H22F3N | − 8.8 | 8 Hydrophobic interactions, 1 H-bond | GLN15C, ALA18C, GLU143D, ILE195B, ARG198B, HIS139D |
Alaway | C23H23NO5S | − 8.7 | 4 Hydrophobic interactions, 1 H-bond | ALA22C, ARG142D, GLU143D, ILE195B, ALA18C |
Zelboraf | C23H18ClF2N3O3S | − 8.7 | 5 Hydrophobic interactions, 3 H-bonds, 1 halogen bond | ALA18C, ALA22C, ALA191B, ILE195B, ASP19C, GLU143D, ARG198B, LYS189B |