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International Archives of Occupational and Environmental Health

Erschienen in:

01.05.2006 | Original Article

Sperm DNA damage correlates with polycyclic aromatic hydrocarbons biomarker in coke-oven workers

verfasst von: Ping-Chi Hsu, I-Yueh Chen, Chih-Hong Pan, Kuen-Yuh Wu, Min-Hsiung Pan, Jenq-Renn Chen, Cheng-Jung Chen, Guo-Ping Chang-Chien, Chang-Hung Hsu, Chiu-Shong Liu, Ming-Tsang Wu

Erschienen in: International Archives of Occupational and Environmental Health | Ausgabe 5/2006

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Abstract

Objectives: The aim was to determine whether occupational exposure to polycyclic aromatic hydrocarbons (PAHs) in men has adverse effect on semen quality. Methods: Forty-eight coke-oven workers, including 16 topside-oven (TO) workers and 32 sideoven (SO) workers, were studied. Ambient PAHs exposure, urinary 1-hydroxypyrene (1-OHP) levels, and parameters of semen quality were determined. Results: TO workers had significantly higher ambient PAHs exposure (3,436.1±3,411.0 vs. 1,123.1±1,829.3 ng/m³, P<0.01), urinary 1-OHP levels (207.8±176.4 vs. 54.0±44.8 μg/g, P<0.001), frequency of oligospermia (18.8 vs. 0%, P<0.05), and morphological abnormality in sperm (32.3 vs. 14.6%, P<0.01) than SO workers. Among cigarette smokers, TO workers had significantly higher rates of DNA denaturation in spermatozoa (αT) and percentage of sperm with increased DNA denaturation (COMP αT) levels than SO workers (246.2±49.5 vs. 198.1±30.3 for αT; 34.8±14.4 vs. 19.3±13.9% for COMP αT, P<0.05). There was a positive correlation between urinary 1-OHP and αT, COMP αT, and abnormal sperm morphology and a tenfold increase in urinary 1-OHP associated with a 2.35-fold increase in αT, as well as a 1.07-fold increase in percentage of sperm with abnormal morphology. Conclusions: A potential risk of sperm dysfunction should be considered for workers occupationally exposed to high levels of PAHs. Cigarette smoking may aggravate this risk. Urinary 1-OHP can be used as a biomarker predicting sperm dysfunction.

Armstrong B, Hutchinson E, Unwin J, Fletcher T (2004) Lung cancer risk after exposure to polycyclic aromatic hydrocarbons: a review and meta-analysis. Environ Health Perspect 112:970–978PubMed

Auger J, Kunstman JM, Czyglik F, Jouannet P (1995) Decline in semen quality among fertile men in Paris during the past 20 years. N Engl J Med 332:281–285CrossRefPubMed

Boogaard PJ, Sittert NJ (1994) Exposure to polycyclic aromatic hydrocarbons in petrochemical industries by measurement of urinary 1-hydroxypyrene. Occup Environ Med 51:250–258PubMed

Boogaard PJ, Sittert NJ (1995) Urinary 1-hydroxypyrene as biomarker of exposure to polycyclic aromatic hydrocarbons in workers in petrochemical industries: baseline values and dermal uptake. Sci Total Environ 163:203–209CrossRefPubMed

Bouchard M, Thuot T, Carrier G, Viau C (2002) Urinary excretion kinetics of 1-hydroxypyrene in rats subchronically exposed to pyrene or polycyclic aromatic hydrocarbon mixtures. J Toxicol Environ Health A 65:1195–1209CrossRefPubMed

Chen ML, Mao IF, Wu MT, Chen JR, Ho CK, Smith TJ, Wypij D, Christiani DC (1999) Assessment of coke oven emissions exposure among coking workers. Am Ind Hyg Assoc J 60:105–110PubMed

Chia SE, Xu B, Ong CN, Tsakok FM, Lee ST (1994) Effect of cadmium and cigarette smoking on human semen quality. Int J Fertil Menopausal Stud 39:292–298PubMed

Dunphy BC, Barratt CL, von Tongelen BP, Cooke ID (1991) Male cigarette smoking and fecundity in couples attending an infertility clinic. Andrologia 23:223–225PubMedCrossRef

Evenson DP, Jost LK, Baer RK, Turner TW, Schrader SM (1991) Individuality of DNA denaturation patterns in human sperm as measured by the sperm chromatin structure assay. Reprod Toxicol 5:115–125CrossRefPubMed

Gaspari L, Chang SS, Santella RM, Garte S, Pedotti P, Taioli E (2003) Polycyclic aromatic hydrocarbon-DNA adducts in human sperm as a marker of DNA damage and infertility. Mutat Res 535:155–160PubMed

Hatjian BJ, Edwards JW, Harrison J, Williams FM, Blain PG (1995) Ambient, biological, and biological effect monitoring of exposure to polycyclic aromatic hydrocarbons (PAHs). Toxicol Lett 77:271–279CrossRefPubMed

Hu CW, Wu MT, Chao MR, Pan CH, Wang CJ, Swenberg JA, Wu KY (2004) Comparison of analyses of urinary 8-hydroxy-2′-deoxyguanosine using isotope-dilution liquid chromatography electrospray tandem mass spectrometry and enzyme-linked immunosorbent assay. Rapid Commun Mass Spectrom 18:505–510CrossRefPubMed

International Agency for Research on Cancer (1984) IARC monograph on the evaluation of carcinogenic risks to humans. Polynuclear aromatic compounds. Part 3. Industrial exposures in aluminum production, coal gasification, coke production, and iron and steel founding. IARC, Lyon

Inyang F, Ramesh A, Kopsombut P, Niaz MS, Hood DB, Nyanda AM, Archibong AE (2003) Disruption of testicular steroidogenesis and epididymal function by inhaled benzo(a)pyrene. Reprod Toxicol 17:527–537CrossRefPubMed

Jongeneelen FJ (1992) Biological exposure limit for occupational exposure to coal tar pitch volatiles at cokeovens. Int Arch Occup Environ Health 63:511–516CrossRefPubMed

Kalcher K, Kern W, Pietsch R (1993) Cadmium and lead in the smoke of a filter cigarette. Sci Total Environ 128:21–35CrossRefPubMed

Kang DH, Rothman N, Poirier MC, Greenberg A, Hsu CH, Schwartz BS, Baser ME, Groopman JD (1995) Interindividual differences in the concentration of 1-hydroxypyrene-glucuronide in urine and polycyclic aromatic hydrocarbon-DNA adducts in peripheral white blood cells after charbroiled beef consumption. Carcinogenesis 16:1079–1085PubMedCrossRef

Keimig SD, Kirby KW, Morgan DP, Keiser JE, Hubert TD (1983) Identification of 1-hydroxypyrene as a major metabolite of pyrene in pig urine. Xenobiotica 13:415–420PubMedCrossRef

Khorram O, Garthwaite M, Jones J, Golos T (2004) Expression of aryl hydrocarbon receptor (AHR) and aryl hydrocarbon receptor nuclear translocator (ARNT) mRNA expression in human spermatozoa. Med Sci Monit 10:BR135–BR138PubMed

Marczynski B, Preuss R, Mensing T, Angerer J, Seidel A, El Mourabit A, Wilhelm M, Bruning T (2005) Genotoxic risk assessment in white blood cells of occupationally exposed workers before and after alteration of the polycyclic aromatic hydrocarbon (PAH) profile in the production material: comparison with PAH air and urinary metabolite levels. Int Arch Occup Environ Health 78:97–108CrossRefPubMed

Ny ET, Heederik D, Kromhout H, Jongeneelen FJ (1993) The relationship between polycyclic aromatic hydrocarbons in air and in urine of workers in a Soderberg potroom. Am Ind Hyg Assoc J 54:277–284PubMed

Pacifici R, Altieri I, Gandini L, Lenzi A, Pichini S, Rosa M, Zuccaro P, Dondero F (1993) Nicotine, cotinine, and trans-3-hydroxycotinine levels in seminal plasma of smokers: effects on sperm parameters. Ther Drug Monit 15:358–363PubMedCrossRef

Paracchini V, Chang S-S, Santella RM, Garte S, Pedotti P, Taioli E (2005) GSMT1 deletion modifies the levels of polycyclic aromatic hydrocarbon–DNA adducts in human sperm. Mutat Res 586:97–101PubMed

Paulozzi LJ, Erickson D, Jackson RJ (1997) Hypospadias trends in two US surveillance systems. Pediatrics 10:831–834CrossRef

Ramesh A, Inyang F, Hood DB, Archibong AE, Knuckles ME, Nyanda AM (2001) Metabolism, bioavailability, and toxicokinetics of benzo(a)pyrene in F-344 rats following oral administration. Exp Toxicol Pathol 53:275–290CrossRefPubMed

Revel A, Raanani H, Younglai E, Xu J, Han R, Savouret J-F, Casper RF (2001) Resveratrol, a natural aryl hydrocarbon receptor antagonist, protects sperm from DNA damage and apoptosis caused by benzo(a)pyrene. Reprod Toxicol 15:479–486CrossRefPubMed

Seleven SG, Borkovec L, Slott VL, Zudova Z, Rubes J, Evenson DP (2000) Semen quality and reproductive health of young Czech men exposed to seasonal air pollution. Environ Health Perspect 108:887–894PubMedCrossRef

Shen HM, Chia SE, Ni ZY, New AL, Lee BL, Ong CN (1997) Detection of oxidative DNA damage in human sperm and association with cigarette smoking. Reprod Toxicol 11:675–680CrossRefPubMed

Smiley ST, Reers M, Mottola-Hartshorn C, Lin M, Chen A, Smith TW, Steele GD Jr, Chen LB (1991) Intracellular heterogeneity in mitochondrial membrane potentials revealed by a J-aggregate-forming lipophilic cation JC-1. Proc Natl Acad Sci USA 88:3671–3675PubMedCrossRef

Sram RJ, Binkova B, Rossner P, Rubes J, Topinka J, Dejmek J (1999) Adverse reproductive outcomes from exposure to environmental mutagens. Mutat Res 428:203–215PubMed

Tas S, Lauwerys R, Lison D (1996) Occupational hazards for the male reproductive system. Crit Rev Toxicol 26:261–307PubMedCrossRef

Tsai PJ, Shieh HY, Lee WJ, Chen HL, Shih TS (2002) Urinary 1-hydroxypyrene as a biomarker of internal dose of polycyclic aromatic hydrocarbons in carbon black workers. Ann Occup Hyg 46:229–235CrossRefPubMed

Vinggaard AM, Hnida C, Larsen JC (2000) Environmental polycyclic aromatic hydrocarbons affect androgen receptor activation in vitro. Toxicology 145:173–183CrossRefPubMed

Wallock LM, Tamura T, Mayr CA, Johnston KE, Ames BN, Jacob RA (2001) Low seminal plasma folate concentrations are associated with low sperm density and count in male smokers and nonsmokers. Fertil Steril 75:252–259CrossRefPubMed

Wang SL, Wang XR, Chia SE, Shen HM, Song L, Xing HX, Chen HY, Ong CN (2001) A study on occupational exposure to petrochemicals and smoking on seminal quality. J Androl 22:73–78PubMed

World Health Organization (2002) WHO laboratory manual for the examination of human semen and seminal–cervical mucus interaction, 3rd edn. WHO, Cambridge University Press, Cambridge

Wu MT (2002) Assessment of the effectiveness of respirator usage in coke oven workers. AIHA J 63:72–75CrossRef

Wu MT, Kelsey KT, Mao IF, Wypij D, Christiani DC (1997) Elevated serum liver enzymes in coke oven and by-product workers. J Occup Environ Med 39:527–533CrossRefPubMed

Wu MT, Mao IF, Ho CK, Wypij D, Lu PL, Smith TJ, Chen ML, Christiani DC (1998) Urinary 1-hydroxypyrene concentrations in coke oven workers. Occup Environ Med 55:461–467PubMedCrossRef

Wu MT, Pan CH, Huang YL, Tsai PJ, Chen CJ, Wu TN (2003) Urinary excretion of 8-hydroxy-2-deoxyguanosine and 1-hydroxypyrene in coke-oven workers. Environ Mol Mutagen 42:98–105CrossRefPubMed

Zitzmann M, Rolf C, Nordhoff V, Schrader G, Rickert-Fohring M, Gassner P, Behre HM, Greb RR, Kiesel L, Nieschlag (2003) Male smokers have a decreased success rate for in vitro fertilization and intracytoplasmic sperm injection. Fertil Steril 79:1550–1554CrossRefPubMed

Titel: Sperm DNA damage correlates with polycyclic aromatic hydrocarbons biomarker in coke-oven workers
verfasst von: Ping-Chi Hsu
I-Yueh Chen
Chih-Hong Pan
Kuen-Yuh Wu
Min-Hsiung Pan
Jenq-Renn Chen
Cheng-Jung Chen
Guo-Ping Chang-Chien
Chang-Hung Hsu
Chiu-Shong Liu
Ming-Tsang Wu
Publikationsdatum: 01.05.2006
Verlag: Springer-Verlag
Erschienen in: International Archives of Occupational and Environmental Health / Ausgabe 5/2006
Print ISSN: 0340-0131
Elektronische ISSN: 1432-1246
DOI: https://doi.org/10.1007/s00420-005-0066-3

Springer Medizin

Abstract

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