Background
Methods
Collection of the plant
Extraction and fractionation of the plant material
Chemicals
Animals
Drugs and Treatment
Acute toxicity study
Neuropharmacological Activity
Thiopental sodium induced sleeping time test
Hole cross test
Open field test
Statistical Analysis
Results
Acute Toxicity
Neuropharmacological Activity
Thiopental sodium induced sleeping time test
Group | Dose (mg/kg) | Onset of Sleep (minutes) | Duration of Sleep (minutes) | Percent effect |
---|---|---|---|---|
Control | 5 ml/kg | 2.06 ± 0.64 | 66.33 ± 8.04 | 100 |
Diazepam | 2 | 1.36 ± 0.06 | 153.5 ± 11.53** | 231.42 |
ACR | 100 | 1.83 ± 0.38 | 83.33 ± 2.36 | 125.63 |
200 | 1.67 ± 0.12 | 183.5 ± 21.40** | 276.65 | |
PEF | 100 | 1.77 ± 0.18 | 105 ± 6.99 | 158.29 |
200 | 1.89 ± 0.43 | 164.17 ± 17.61** | 247.50 | |
CHF | 100 | 1.87 ± 0..25 | 97.67 ± 20.08 | 147.25 |
200 | 1.28 ± 0.22 | 237.83 ± 8.09** | 358.55 | |
MEF | 100 | 1.92 ± 0.23 | 95.17 ± 16.25 | 143.48 |
200 | 1.51 ± 0.13 | 176.83 ± 24.57** | 266.59 | |
ACL | 100 | 1.91 ± 0.24 | 69.5 ± 4.09 | 104.78 |
200 | 1.41 ± 0.06 | 113.5 ± 15.65* | 171.11 |
Hole cross test
Group | Dose (mg/kg) | Number of movements (% of Number of movements inhibition) | ||||
---|---|---|---|---|---|---|
0 min | 30 min | 60 min | 90 min | 120 min | ||
Control | 5 ml/kg | 15.5 ± 1.84 | 14.33 ± 2.18 | 13.67 ± 1.83 | 10.5 ± 1.03 | 10 ± 1.41 |
Diazepam | 2 | 15 ± 1.09 | 7.34 ± 0.76 (48.78 %) | 4.67 ± 0.61** (65.83 %) | 3.83 ± 0.75** (63.53 %) | 2.83 ± 0.59** (71.70 %) |
ACR | 100 | 7.5 ± 2.36 | 2.83 ± 1.01** (80.25 %) | 2.33 ± 0.76** (82.95 %) | 1.83 ± 0.47** (82.57 %) | 3.33 ± 1.31* (66.7 %) |
200 | 9.67 ± 1.76 | 1.83 ± 0.31** (87.23 %) | 0.83 ± 0.31** (93.93 %) | 1.5 ± 0.43** (85.72 %) | 1.67 ± 0.88** (83.3 %) | |
PEF | 100 | 11.33 ± 2.47 | 1.17 ± 0.31** (91.83 %) | 1.17 ± 0.40** (91.44 %) | 2 ± 0.82** (80.95 %) | 2.5 ± 1.05** (75 %) |
200 | 8 ± 1.31 | 1 ± 0.26** (93.02 %) | 0.83 ± 0.47** (93.93 %) | 1.67 ± 0.61** (84.09 %) | 1.17 ± 0.60** (88.3 %) | |
CHF | 100 | 10.83 ± 0.65 | 4.83 ± 3.26* (66.29 %) | 2.5 ± 1.70** (81.71 %) | 3.67 ± 0.84** (65.05 %) | 2.17 ± 0.60** (78.3 %) |
200 | 11.17 ± 3.09 | 4.5 ± 1.41* (68.59 %) | 1.83 ± 1.07** (86.61 %) | 1.5 ± 0.95** (85.71 %) | 0.67 ± 0.33** (93.3 %) | |
MEF | 100 | 8 ± 1.41 | 5.67 ± 2.82* (60.43 %) | 2 ± 1.06** (85.37 %) | 1.33 ± 0.71** (87.33 %) | 1.67 ± 0.61** (83.3 %) |
200 | 11 ± 1.21 | 2.83 ± 1.19* (80.25 %) | 1.83 ± 0.54** (86.61 %) | 1.33 ± 0.49** (87.33 %) | 1.5 ± 0.50** (85.00 %) | |
ACL | 100 | 9.67 ± 1.99 | 2.5 ± 0.72** (82.55 %) | 2.67 ± 0.95** (80.47 %) | 2.5 ± 0.67** (76.19 %) | 1.83 ± 4.00** (81.7 %) |
200 | 6.83 ± 2.01 | 1.33 ± 0.95** (90.72 %) | 0.67 ± 0.49** (95.09 %) | 1 ± 0.52** (90.47 %) | 0.5 ± 0.34** (95.00 %) |
Open field test
Group | Dose (mg/kg) | Number of movements (% of Number of movements inhibition) | ||||
---|---|---|---|---|---|---|
0 min | 30 min | 60 min | 90 min | 120 min | ||
Control | 5 ml/kg | 112.83 ± 6.24 | 112.5 ± 5.67 | 103.33 ± 11.39 | 99 ± 3.95 | 90.67 ± 1.52 |
Diazepam | 2 | 113 ± 2.62 | 57.83 ± 9.68* (48.59 %) | 46.67 ± 11.28* (54.83 %) | 37.83 ± 11.67** (61.78 %) | 26.67 ± 14.33** (70.58 %) |
ACR | 100 | 64.83 ± 17.24 | 25.17 ± 12.4** (77.62 %) | 18 ± 4.76** (82.58 %) | 23.67 ± 7.54** (76.09 %) | 27 ± 6.76** (70.22 %) |
200 | 91 ± 8.97 | 15.17 ± 4.73** (86.52 %) | 6.5 ± 2.38** (93.71 %) | 10.83 ± 3.58** (89.06 %) | 10.83 ± 3.98** (88.05 %) | |
PEF | 100 | 70.17 ± 11.86 | 14 ± 7.06** (87.55 %) | 12.17 ± 4.46** (88.22 %) | 11 ± 4.26** (88.88 %) | 17.33 ± 4.94** (80.88 %) |
200 | 86.67 ± 11.14 | 13.83 ± 7.89** (87.71 %) | 9.5 ± 1.38** (90.80 %) | 9 ± 3.03** (90.90 %) | 6.5 ± 3.04** (92.83 %) | |
CHF | 100 | 90 ± 7.19 | 31.5 ± 5.19** (72.00 %) | 24.33 ± 9.36** (76.45 %) | 25.83 ± 5.28** (73.90 %) | 15.33 ± 5.94** (83.09 %) |
200 | 104.83 ± 8.18 | 12 ± 4.84** (89.33 %) | 11.67 ± 6.51** (88.70 %) | 11.67 ± 6.02** (87.21 %) | 13.83 ± 6.99** (84.75 %) | |
MEF | 100 | 84.17 ± 12.54 | 32.67 ± 17.79* (70.96 %) | 21.33 ± 11.97** (79.36 %) | 13.17 ± 3.50** (86.69 %) | 10.83 ± 7.06** (88.05 %) |
200 | 90.83 ± 16.28 | 19.83 ± 7.66** (82.37 %) | 12 ± 3.71** (88.38 %) | 10.33 ± 3.67** (89.56 %) | 10.83 ± 3.98** (88.05 %) | |
ACL | 100 | 71.83 ± 22.56 | 44.83 ± 18.22* (60.15 %) | 26.83 ± 18.57** (74.03 %) | 11.33 ± 10.73** (88.55 %) | 6.33 ± 6.32** (93.01 %) |
200 | 79.67 ± 11.34 | 31 ± 11.12* (72.44 %) | 16.17 ± 3.49** (84.35 %) | 6 ± 0.77** (93.94 %) | 4 ± 1.26** (95.58 %) |