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Erschienen in:
01.03.2015 | Short Communication
Synchrotron-based X-ray tomographic microscopy for visualization of three-dimensional collagen matrices
Erschienen in: Clinical Oral Investigations | Ausgabe 2/2015
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Introduction
Three-dimensional collagen matrices (3D-CMs) may be visualized by cumbersome reconstructions of serial sections. We report here on the method of synchrotron-based X-ray tomographic microscopy (SRXTM) to image 3D-CMs in native tissue probes.
Material and methods
SRXTM of 3D-CMs (mucoderm®, mucograft®) was performed at the TOMCAT beamline of the Swiss Light Source (SLS) at the Paul Scherrer Institute (Villigen, Switzerland).
Results
SRXTM combines the advantages of high-resolution scanning electron microscopy (SEM) imaging with the low-resolution reconstructions of micro-CT (μCT) imaging. It may be used to non-destructively visualize and analyze structures within the 3D-CMs without the need of serial sectioning and reconstruction.
Conclusion
High-resolution SRXTM is a useful tool in analyzing the topology and morphometry of structures in 3D-CMs. The outcome justifies the efforts in sophisticated data processing.
Clinical relevance
SRXTM may help to understand the clinical characteristics of 3D-CMs in more detail.