Systemic therapy for advanced clear cell renal cell carcinoma after discontinuation of immune-oncology and VEGF targeted therapy combinations

Renal cell carcinoma (RCC) is among the top ten most common cancers worldwide with an annual incidence of approximately 65,000 new cases in the United States [1]. An important landmark in the management landscape of metastatic RCC was the Food and Drug Administration (FDA) approval in 2015 of nivolumab, an Immune-Oncology (IO) agent targeting the Programmed Death-1(PD-1) axis based on the Checkmate 025 phase 3 clinical trial [2]. Since then, preclinical data supported the rationale for developing IO-based combinations [3, 4] to improve the clinical outcomes of single agent IO. Such combination approaches included the dual IO combination regimen of ipilimumab and nivolumab which received regulatory approval by the FDA in 2018 as a first-line therapy in patients with intermediate and poor risk metastatic clear cell RCC (ccRCC) based on the phase 3 Checkmate 214 clinical trial [5]. Other studied combinations are the combinations of IO with Vascular Endothelial Growth Factor (VEGF) targeted therapies (IO-VEGF) including IO with VEGF receptor (VEGFR) tyrosine kinase inhibitors (IO-TKI) and IO with the anti-VEGF monoclonal antibody bevacizumab (IO-Bev). Promising results of these combinations were reported in large randomized phase 3 clinical trials in comparison to sunitinib. IMmotion 151 was the first phase 3 clinical trial reporting positive results of a combination of IO with VEGF targeted therapy [6]. In this study the combination of atezolizumab with bevacizumab showed superior progression free survival (PFS) but not in overall survival (OS) compared to sunitinib in previously untreated metastatic ccRCC with PD-L1 positive disease. KEYNOTE-426 [7] investigated the combination of pembrolizumab with axitinib in the first-line setting in patients with metastatic ccRCC, this combination demonstrated improved OS and PFS regardless of the International Metastatic Database Consortium (IMDC) risk group and PD-L1 status compared to sunitinib. JAVELIN Renal 101 [8] investigated the combination of avelumab with axitinib in the first-line setting and demonstrated improved PFS compared to sunitinib, with immature OS results to date. The positive results of KEYNOTE-426 and JAVELIN Renal 101 have led to the regulatory approval of both combinations (axitinib/pembrolizumab and axitinib/avelumab) in patients with metastatic ccRCC in the first-line setting [9, 10]. Currently, several other novel IO based combinations are under study in metastatic RCC [11‐13].

However, outcomes of patients treated with anti-kidney cancer systemic therapy after discontinuation of IO-VEGF combinations remain poorly understood and mainly based on few small retrospective series [14‐17]. Herein, we report the clinical outcomes to subsequent systemic therapy in a multi-institutional analysis of patients with metastatic ccRCC who discontinued IO-VEGF combinations.

The discovery of IO agents [19, 20] has led to remarkable progress in the RCC treatment landscape with the regulatory approval of anti-PD-1 monotherapy (nivolumab) as the first FDA approved IO in RCC [2]. To increase efficacy and overcome resistance mechanisms, preclinical evidence has suggested novel combinatory approaches [3, 4]. The combination of VEGF and PD-1/PD-L1 directed therapies has been studied in several phase 3 clinical trials including the combination of atezolizumab /bevacizumab (IMmotion 151 trial), pembrolizumab/axitinib (KEYNOTE-426), avelumab/axitinib (JAVELIN Renal 101), pembrolizumab/lenvatinib (CLEAR trial), and nivolumab/cabozantinib (CheckMate 9ER trial). Positive results from KEYNOTE-426 [7] and JAVELIN Renal 101 [8] have led to the regulatory approval of pembrolizumab/axitinib [9] and avelumab/axitinib [10] as a standard first-line treatment for patients with metastatic ccRCC, and the results from the CLEAR trial and CheckMate 9ER remain pending. However, despite these promising results, for many patients subsequent therapy remains necessary after discontinuation of IO-VEGF combinations. In this study we conducted a multi-institutional retrospective analysis to examine the efficacy of subsequent systemic therapy after discontinuation of IO-VEGF combinations.

Our study, which was originally presented at the ASCO Annual Meeting, represents the largest study to date of patients who were treated after discontinuation of IO-VEGF combinations [21]. Post discontinuation of IO-VEGF combinations, the majority of patients in our study received cabozantinib or axitinib (n = 40; 68%), with the remaining patients receiving other kidney cancer systemic therapies. The activity of cabozantinib and axitinib in previously treated patients with metastatic ccRCC was demonstrated in the METEOR [22] and AXIS [23] randomized phase 3 clinical trials, respectively. The METEOR study [22] examined clinical outcomes of cabozantinib in comparison to everolimus in 658 patients with metastatic ccRCC after progression on prior therapy and reported a median OS of 21.4 months (95% CI, 18.7–NE), PFS of 7.4 months (95% CI, 6.6–9.1) and ORR of 17% for patients treated with cabozantinib. For axitinib, the AXIS study [23] examined clinical outcomes of axitinib in comparison to sorafenib in 723 patients with metastatic ccRCC after progressing on prior therapy and reported a median OS of 20.1 months (95% CI, 16.7–23.4), median PFS of 6.7 months (95% CI, 6.3–8.6) and ORR of 19% for patients treated with axitinib. However, none of the patients on the axitinib arm on the AXIS study [23] received prior IO and only 5% of patients on the cabozantinib arm on the METEOR study [22] received prior IO. In this retrospective study of patients who were previously treated with IO-VEGF combinations, we demonstrated clinical efficacy that compared favorably to historic controls with a median OS of 24.5 months (95% CI, 12.0–NE), median PFS of 12 months (95% CI, 8.2–24.5) and ORR of 25%. These results may be partially explained by the limited number of patients that were available for evaluation. Also of note, 60% of the patients in this cohort were treated with bevacizumab containing combinations, and bevacizumab is less commonly used in the first-line setting; however, outcomes to subsequent therapy were similar when comparing patients with IO-TKI and IO-Bev. It is also possible that patients continued to derive some benefit from prior IO-VEGF combinations even after the discontinuation of the IO agent in view of the short time interval between discontinuation of IO-VEGF and starting the next line of therapy (Median 28 days, range 3–574).

With regards to the activity of VEGF targeted therapy after prior treatment with IO, Ornstein et al. [24] recently reported results from a phase 2 multicentre study on clinical outcomes of 40 patients who were treated with axitinib in an individualized dosing algorithm after receiving IO therapy as the most recent treatment including IO monotherapy or in combination with VEGF targeted therapy with PFS as the primary endpoint. In this study; 71% of patients received IO monotherapy and 5% received IO-VEGF combinations. Axitinib treatment was associated with high clinical activity (45% ORR with 67% of responses lasting > 12 months and a PFS of 8.8 months). Lee et al. recently reported interim analysis of a phase 2 multicentre study which examined the question of salvage therapy with IO based combination after prior IO failure [25] (NCT02501096). In this study, 33 patients who progressed on IO treatment received pembrolizumab and lenvatinib combination, which was associated with promising results in this setting with ORR of 64% and 11.3 months PFS indicating that salvage therapy after IO failure with a combination of IO and VEGF targeted therapy is a feasible approach. Other retrospective studies evaluating the efficacy of VEGFR-TKI agents after failure of front line IO based combination therapies have also reported clinical activity that is possibly impacted by prior therapy. Barata et al. [14] reported a median PFS of 6.4 months (95% CI, 4.4–8.4) and ORR of 29% in a cohort of 33 patients who were treated with VEGFR-TKI after progressing on IO-based combinations (atezolizumab/bevacizumab, ipilimumab/nivolumab, avelumab/axitinib). Auvray et al. [26] reported a median PFS of 8.0 months (95% CI, 5.0–13.0), 12- months survival rate of 54%, and ORR of 36% in a cohort of 33 patients treated with VEGFR TKI after failure of ipilimumab with nivolumab. However, a larger percentage of patients in the aforementioned studies received first generation VEGF-TKIs with sunitinib or pazopanib as the next subsequent therapy (13/33 patients [39%]; Barata et al. and 23/33 patients [70%]; Auvray et al.) compared to our study (6/55 patients [11%]) which may partially explain the differences in outcomes. Furthermore, another retrospective study examined outcomes of 70 patients treated with VEGF-TKI after prior IO therapy (including 36% patients who received IO-VEGF combinations) and showed similar results to our findings with a median PFS of 13.2 months (95% CI, 10.1–NA), 12-months survival rate of 79.6% (95% CI, 70.2–90.3), and ORR of 41% [15].

Some of the limitations of our study include the retrospective design, small sample size, and the relatively short follow-up time. Moreover, the majority of patients included in the efficacy analysis (n = 32, 58%) received atezolizumab and bevacizumab combination, which was not associated with OS benefit in the overall population in the IMmotion 151 phase III clinical trial (HR 0.93, 95% CI 0.76–1.14, p = 0.47). This combination is currently thought not to be as active compared to IO-VEGF TKI based combinations; thus, this possibly could be driving the favorable outcomes in our study. Furthermore, in view of the multicentre retrospective nature of the study, specific study population data were not collected, including the number of patients with tumors harboring sarcomatoid features, details of metastatic sites, treatment-related toxicity data and the doses of VEGF-R TKI used post IO-VEGF. It is also reasonable to postulate that the favorable outcomes observed in our study might be influenced by the type of therapy received (68% received cabozatinib or axitinib) and that almost all patients were treated with IO-VEGF combinations as part of a clinical trial; suggestive of a favorable population which may limit the applicability of our study findings. Nonetheless, despite these limitations, our study represents one of the largest reports in this setting, providing further evidence on systemic therapy outcomes post-IO-VEGF.

Our findings indicate continued efficacy of systemic therapy after discontinuation of IO-VEGF combinations which is suggestive of possible continued benefit from prior IO therapy and/or maintaining sensitivity to VEGF directed therapy. Further studies are necessary to validate these findings.