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European Journal of Nuclear Medicine and Molecular Imaging

Erschienen in:

01.02.2015 | Original Article

Targeting post-infarct inflammation by PET imaging: comparison of ⁶⁸Ga-citrate and ⁶⁸Ga-DOTATATE with ¹⁸F-FDG in a mouse model

verfasst von: James T. Thackeray, Jens P. Bankstahl, Yong Wang, Mortimer Korf-Klingebiel, Almut Walte, Alexander Wittneben, Kai C. Wollert, Frank M. Bengel

Erschienen in: European Journal of Nuclear Medicine and Molecular Imaging | Ausgabe 2/2015

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Abstract

ᅟ

Imaging of inflammation early after myocardial infarction (MI) is a promising approach to the guidance of novel molecular interventions that support endogenous healing processes. ¹⁸F-FDG PET has been used, but may be complicated by physiological myocyte uptake. We evaluated the potential of two alternative imaging targets: lactoferrin binding by ⁶⁸Ga-citrate and somatostatin receptor binding by ⁶⁸Ga-DOTATATE.

Methods

C57Bl/6 mice underwent permanent coronary artery ligation. Serial PET imaging was performed 3 – 7 days after MI using ⁶⁸Ga-citrate, ⁶⁸Ga-DOTATATE, or ¹⁸F-FDG with ketamine/xylazine suppression of myocyte glucose uptake. Myocardial perfusion was evaluated by ¹³N-ammonia PET and cardiac geometry by contrast-enhanced ECG-gated CT.

Results

Mice exhibited a perfusion defect of 30 – 40 % (of the total left ventricle) with apical anterolateral wall akinesia and thinning on day 7 after MI. ¹⁸F-FDG with ketamine/xylazine suppression demonstrated distinct uptake in the infarct region, as well as in the border zone and remote myocardium. The myocardial standardized uptake value in MI mice was significantly higher than in healthy mice under ketamine/xylazine anaesthesia (1.9 ± 0.4 vs. 1.0 ± 0.1). ⁶⁸Ga images exhibited high blood pool activity with no specific myocardial uptake up to 90 min after injection (tissue-to-blood contrast 0.9). ⁶⁸Ga-DOTATATE was rapidly cleared from the blood, but myocardial SUV was very low (0.10 ± 0.03).

Conclusion

Neither ⁶⁸Ga nor ⁶⁸Ga-DOTATATE is a useful alternative to ¹⁸F-FDG for PET imaging of myocardial inflammation after MI in mice. Among the three tested approaches, ¹⁸F-FDG with ketamine/xylazine suppression of cardiomyocyte uptake remains the most practical imaging marker of post-infarct inflammation.

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Titel: Targeting post-infarct inflammation by PET imaging: comparison of 68Ga-citrate and 68Ga-DOTATATE with 18F-FDG in a mouse model
verfasst von: James T. Thackeray
Jens P. Bankstahl
Yong Wang
Mortimer Korf-Klingebiel
Almut Walte
Alexander Wittneben
Kai C. Wollert
Frank M. Bengel
Publikationsdatum: 01.02.2015
Verlag: Springer Berlin Heidelberg
Erschienen in: European Journal of Nuclear Medicine and Molecular Imaging / Ausgabe 2/2015
Print ISSN: 1619-7070
Elektronische ISSN: 1619-7089
DOI: https://doi.org/10.1007/s00259-014-2884-6

Springer Medizin

Abstract

ᅟ

Methods

Results

Conclusion

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