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Medical Oncology

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01.06.2012 | Original Paper

The analysis of CD45 isoforms expression on HBV-specific T cells after liver transplantation

verfasst von: Lelin Pan, Wenjin Zhang, Jian Zhang, Lin Zhou, Lanjuan Li, Shusen Zheng

Erschienen in: Medical Oncology | Ausgabe 2/2012

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Abstract

This study aimed to analyze the expression of CD45 on HBV-specific T cells in patients who underwent liver transplantation (LT). We detected the distribution of two CD45 isoforms (CD45RA, CD45RO) and CD27 on peripheral blood lymphocytes including two HBV-specific pentamer cells by flow cytometry. Meanwhile, the levels of tacrolimus, plasma IL-7, and IL-15 were tested by ELISA. CD45RA decreased sharply after LT, whereas the expression of CD45RO⁺ changed a very little. The proportion of CD4⁺ CD27⁺ subsets increased in the span from first 6–36 months after LT. With the up-regulation of IL-7, CD45RA⁺ CD27⁺ was reduced and CD45RO⁺ was increased on HBV-specific T cells in LT group compared with CHB. Therefore, the switch from CD45RA to CD45RO probably depended on IL-7, without being stimulated by hepatitis B virus (HBV) and proliferated by IL-15. IL-7 could aid in maintaining HBV-specific T cells effectively during 3 years.

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Titel: The analysis of CD45 isoforms expression on HBV-specific T cells after liver transplantation
verfasst von: Lelin Pan
Wenjin Zhang
Jian Zhang
Lin Zhou
Lanjuan Li
Shusen Zheng
Publikationsdatum: 01.06.2012
Verlag: Springer US
Erschienen in: Medical Oncology / Ausgabe 2/2012
Print ISSN: 1357-0560
Elektronische ISSN: 1559-131X
DOI: https://doi.org/10.1007/s12032-011-9833-z

Springer Medizin

The analysis of CD45 isoforms expression on HBV-specific T cells after liver transplantation

Abstract

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