Introduction
Materials and methods
Experimental design
External mechanical loading
High-resolution micro-computed tomography analysis
Statistical analysis
Results
Effects of NS-398 on body weight and bone length
Effects of NS-398 on trabecular and cortical bone
Vehicle + control | Vehicle + loading | NS-398 + control | NS-398 + loading | NS-398 |
P value loading | Interaction | |
---|---|---|---|---|---|---|---|
Trabecular bone of the proximal tibia | |||||||
Bone volume/tissue volume (%) | 17.5 ± 0.5 | 24.7 ± 0.9 | 16.4 ± 0.3 | 22.4 ± 0.4 |
0.008
|
<0.001
| 0.355 |
Trabecular number (mm−1) | 3.30 ± 0.08 | 3.71 ± 0.10 | 3.10 ± 0.05 | 3.44 ± 0.05 |
0.004
|
<0.001
| 0.644 |
Trabecular thickness (μm) | 52.9 ± 0.6 | 66.5 ± 0.9 | 52.7 ± 1.1 | 65.2 ± 1.0 | 0.441 |
<0.001
| 0.559 |
Cortical bone of the tibia | |||||||
Proximal site | |||||||
Bone volume (mm3) | 0.403 ± 0.006 | 0.543 ± 0.010 | 0.411 ± 0.008 | 0.544 ± 0.011 | 0.642 |
<0.001
| 0.682 |
Periosteally enclosed volume (mm3) | 0.713 ± 0.011 | 0.840 ± 0.012 | 0.741 ± 0.012 | 0.847 ± 0.014 | 0.170 |
<0.001
| 0.397 |
Medullary volume (mm3) | 0.309 ± 0.007 | 0.297 ± 0.006 | 0.330 ± 0.007 | 0.303 ± 0.008 | 0.079 |
0.013
| 0.347 |
Proximal/middle site | |||||||
Bone volume (mm3) | 0.378 ± 0.003 | 0.518 ± 0.010 | 0.386 ± 0.007 | 0.515 ± 0.010 | 0.761 |
<0.001
| 0.480 |
Periosteally enclosed volume (mm3) | 0.614 ± 0.008 | 0.749 ± 0.008 | 0.626 ± 0.011 | 0.746 ± 0.010 | 0.638 |
<0.001
| 0.448 |
Medullary volume (mm3) | 0.236 ± 0.007 | 0.230 ± 0.002 | 0.240 ± 0.006 | 0.231 ± 0.005 | 0.692 | 0.144 | 0.751 |
Middle site | |||||||
Bone volume (mm3) | 0.297 ± 0.004 | 0.381 ± 0.004 | 0.309 ± 0.006 | 0.386 ± 0.010 | 0.208 |
<0.001
| 0.554 |
Periosteally enclosed volume (mm3) | 0.483 ± 0.007 | 0.553 ± 0.006 | 0.495 ± 0.010 | 0.558 ± 0.011 | 0.319 |
<0.001
| 0.706 |
Medullary volume (mm3) | 0.186 ± 0.004 | 0.171 ± 0.004 | 0.186 ± 0.005 | 0.172 ± 0.004 | 0.939 |
0.002
| 0.885 |
Distal site | |||||||
Bone volume (mm3) | 0.274 ± 0.004 | 0.272 ± 0.004 | 0.280 ± 0.008 | 0.274 ± 0.006 | 0.474 | 0.475 | 0.747 |
Periosteally enclosed volume (mm3) | 0.371 ± 0.005 | 0.373 ± 0.005 | 0.382 ± 0.009 | 0.381 ± 0.010 | 0.211 | 0.952 | 0.862 |
Medullary volume (mm3) | 0.097 ± 0.002 | 0.102 ± 0.003 | 0.102 ± 0.002 | 0.107 ± 0.004 | 0.074 | 0.102 | 0.825 |
Cortical bone of the fibula | |||||||
Middle site | |||||||
Bone volume (mm3) | 0.0523 ± 0.0009 | 0.0664 ± 0.0021 | 0.0511 ± 0.0006 | 0.0657 ± 0.0019 | 0.516 |
<0.001
| 0.878 |
Periosteally enclosed volume (mm3) | 0.0587 ± 0.0014 | 0.0719 ± 0.0020 | 0.0562 ± 0.0005 | 0.0704 ± 0.0015 | 0.188 |
<0.001
| 0.712 |
Medullary volume (mm3) | 0.0065 ± 0.0006 | 0.0054 ± 0.0003 | 0.0051 ± 0.0003 | 0.0048 ± 0.0006 | 0.054 | 0.160 | 0.527 |