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01.02.2014 | Urology - Original Paper

The effects of preoperative immunosuppressive therapy on ischemia and reperfusion (I/R) injury in healthy rats

verfasst von: Selcuk Kilinc, Sedat Tan, Efsun Hatice Kolatan, Dane Ruscuklu, Esra Satici, Mehmet Kemiksiz, Lutfi Dalkilic, Umut Eren Erdogdu, Cezmi Karaca

Erschienen in: International Urology and Nephrology | Ausgabe 2/2014

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Abstract

Purpose

Warm-ischemia-induced injuries might be encountered during renal transplants from cadavers and healthy donors. Toll-like receptors (TLR) in ischemia–reperfusion (I/R) injury are one of the indicators of intracellular injury pathways. The intensity of ischemic injury is directly proportionate to high TLR levels. To minimize the I/R injury, we investigated TLR2 and TLR4 levels on rats, which were pretreated with tacrolimus (FK506) before I/R.

Methods

Eight Wistar albino rats in the study group were administered .01 mg/kg intramuscular tacrolimus. Administration to the study group was performed 24 and 1 h before warm ischemia. Eight rats in the control group were injected with 0.1 c.c. of distilled water. Blood samples were collected from the tail veins of all the rats on the first, second and third days. Expression levels of TLR2 and TLR4 genes were analyzed using the polymerase chain reaction method, to determine any significant difference between the control and study groups on the days when blood was taken.

Results

TLR2 (p = 0.045) and TLR4 (p = 0.022) levels in the study group were found to be statistically, and significantly, lower than those in the control group, on the second day following warm-ischemia- and reperfusion-induced injury.

Conclusions

Administration of immunosuppressive drugs to healthy donor rats led to a statistically significant reduction in the expression levels of TLR2 and TLR4 in the early period. In light of the data obtained by this study, we hypothesize that a preoperative therapy on donors might have a role in preventing I/R injury.

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Titel: The effects of preoperative immunosuppressive therapy on ischemia and reperfusion (I/R) injury in healthy rats
verfasst von: Selcuk Kilinc
Sedat Tan
Efsun Hatice Kolatan
Dane Ruscuklu
Esra Satici
Mehmet Kemiksiz
Lutfi Dalkilic
Umut Eren Erdogdu
Cezmi Karaca
Publikationsdatum: 01.02.2014
Verlag: Springer Netherlands
Erschienen in: International Urology and Nephrology / Ausgabe 2/2014
Print ISSN: 0301-1623
Elektronische ISSN: 1573-2584
DOI: https://doi.org/10.1007/s11255-013-0548-2

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