The prebiotic effects of soluble dietary fiber mixture on renal anemia and the gut microbiota in end-stage renal disease patients on maintenance hemodialysis: a prospective, randomized, placebo-controlled study

Anemia is one of the common and serious complications of end-stage renal disease (ESRD), and it affects approximately 41% of ESRD patients [1]. Renal anemia is one of the important factors for heart failure and mortality in ESRD, which seriously affect the quality of life of ESRD patients [2]. Dietary fiber (DF) has been shown to improve anemia at the clinical and animal model levels. Carvalho et al. found that partially hydrolyzed guar gum (PHGG), a DF, was able to ameliorate anemia in rats by improving iron metabolism [3]. Additionally, Paganini et al. found that galactooligosaccharides (GOS), one type of DF, were able to improve iron absorption and improve anemia in humans [4]. Recently, a systematic review by Thaísa et al. showed that although there is heterogeneity in the conclusions of numerous current basic and clinical studies on DF for the improvement of anemia associated with iron metabolism, its potential role in the improvement of anemia and intestinal biomarkers should be emphasized [5]. Thus, further studies in this area are still needed.

DF is a polysaccharide derived mainly from plant-based foods that cannot be directly broken down and utilized by the human digestive system. Howerer, DF can serve as a fermentation substrate for gut microbiota residents [6, 7]. DF is divided into soluble DF and insoluble DF. Insoluble DF cannot be dissolved in water, and its main function is to store water in the colon, soften feces, and enhance bowel motility. Thus, DF has a certain therapeutic effect on constipation [8, 9]. Soluble DF is a type of DF that can dissolve in water; these include inulin, oligofructose, resistant starch, guar gum, etc. Humans do not have the genes to degrade polysaccharides, but bacteria can break them down. Therefore, fiber can be utilized by the human body as fermentation products, i.e., short-chain fatty acids (SCFAs). Some bacteria in the human gut are able to use DF as a catabolic substrate to produce substances such as SCFAs and bile acids, which is also one of the reasons why soluble DFs are considered to have prebiotic effects. Studies have reported that decreased SCFA levels in feces and/or serum are associated with a variety of diseases, including anemic disorders [10, 11].

There is heterogeneity in the results of some studies investigating the effects of DF on the treatment of anemia. These differences may be due to differences in different DF species but also by individual gene variability. Differences in the availability of resistant starch due to individual amylase gene heterogeneity were recently described by Dobranowski et al. [12]. However, because DF is not directly utilized by the human body, its catabolism is dependent on certain gut microbiota, such as Akkermansia muciniphila and Clostridium butyricum. Therefore, differences in the gut microbiota may also be one of the reasons for the different effects of DF. Deehan et al. found that arabinoxylan can be fermented by Dialister invisus and Bacteroides plebeius to produce propionic acid to improve insulin resistance, while the bile acid produced by the bacteria can improve intestinal inflammation [13]. Comparatively, DF also influences the gut microbiota and is able to remodel and ameliorate the disordered gut microbiota. Additionally, the increase in beneficial bacteria may in turn better utilize DF to produce beneficial substances such as SCFA. Ranaivo et al. showed that DF is able to modulate the gut microbiota structure and improve its metabolic profile in patients at risk for heart disease [14]. Moreover, an ameliorative effect of a composite DF diet on metabolic diseases was described by Cani et al. [15]. The effect of DF on whole-body metabolism is achieved by the gut microbiota, which metabolizes DF into substances such as SCFA. Xu et al. found that acetate could directly stimulate erythropoiesis through a hypoxia inducible factor-2 (HIF-2)-related pathway [16]. A study by Reid et al. found that butyric acid (BA) derivatives ameliorated sickle cell anemia [17]. Weinberg et al. reported that BA was able to promote the synthesis of γ-globin [18]. However, a study by Soriano-Lerma et al. found that propionate, BA and their related producing bacteria are effective against iron deficiency anemia [10]. The above studies indicate that SCFAs and the gut microbiota are able to affect anemia-related diseases through multiple pathways and are worthy of further study.

The aim of this study was to explore the role of DF, the gut microbiota and SCFAs in renal anemia and to preliminarily explore the possible mechanism underlying this effect using a combination of genomics and metabolomics. We hypothesized that adequate daily intake of DF would improve renal anemia in Chinese participants by modulating the gut microbiota and increasing the concentration of SCFAs. This study explores the role of DF in ameliorating renal anemia. Moreover, this study links DF, the gut microbiota, SCFA, and anemia in an attempt to provide new insights into this regimen of DF modulation of the gut microbiota to ameliorate renal anemia.

Renal anemia is a common and serious complication in ESRD patients and seriously threatens the safety of ESRD patients. Causes of renal anemia include decreased erythropoietin (EPO) production, iron dyshomeostasis, chronic inflammation, reduced red blood cell lifespan due to uremic toxins, and hematopoietic microenvironment disturbances, among others. With the application of rhEPO in the clinic, renal anemia has been effectively treated. However, an increasing number of patients also present clinically with EPO hyporesponse, which may be related to factors such as iron metabolism disorder and EPO receptor dysfunction. Recently, with the application of hypoxia inducible factor prolyl hydroxylase inhibitor (HIF-PHI) in the clinic, the standard rate of treatment of renal anemia in ESRD dialysis patients has been further improved [23, 24]. However, renal anemia is a multifactorial disease, and the diversity of its etiologies has contributed to its need for multitarget therapies.

Through this prospective, randomized, placebo-controlled clinical study, we showed that sufficient DF uptake improved Hb levels in patients with renal anemia. This finding is similar to the results reported in some previous animal studies and clinical studies [3, 25‐28]. Moreover, our results showed that DF consumption notably elevated Hb levels in patients with renal anemia, with an average increase above 20%. This means that for renal anemia patients with a severe deficiency in DF intake, increasing DF consumption could have considerable therapeutic efficacy. In addition, we found that DF could change the gut microbiota structure of patients with renal anemia. For example, the anundance of Bifidobacterium adolescentis, Lactobacillus, and Lactobacillaceae, were increased, and the beneficial effects of Bifidobacterium adolescentis, Lactobacillus, and Lactobacillaceae on CKD patients have been demonstrated [29, 30]. Moreover, our study also found that oral DF increased serum SCFA (especially BA) concentrations in patients with renal anemia. Bifidobacterium adolescentis, Lactobacillus, and Lactobacillaceae are well-established as BA-production-related bacteria, and our results suggest that the effect of DF elevating Hb in patients with renal anemia may be associated with an increase in BA and BA-production-related bacteria [31‐33]. BA is one of the most widely studied SCFAs and has been shown to exist in multiple mechanisms affecting anemia-related diseases. A study by Reid et al. found that BA derivatives ameliorated sickle cell anemia [17]. Weinberg et al. found that BA can promote the synthesis of γ-globin to improve thalassemia [18]. However, as renal anemia is a disease with multiple causes, it is unclear whether BA can improve this condition. Our study confirmed for the first time that BA produced by DF fermented by gut microbiota can improve renal anemia in a clinical trial. Correspondingly, we found that after DF intervention, the abundance of some potential pathogenic bacteria decreased. These species included Acinetobacter and Ruminococcaceae, which are related to infection, hemolysis and anorexia nervosa [34, 35]. However, we found that the relative abundance of Blautia, which is a BA-producing bacterium, was decreased in the DF group, but the serum BA level was elevated. It seems that there is a contradiction. We speculate that this may be related to the ability of Bifidobacterium adolescentis, Lactobacillus, and Lactobacillaceae to enhance butyrate production by other butyrate-producing bacteria. The specific mechanism needs further study.

Most of the current studies on the improvement of anemia by DF have focused on iron deficiency anemia. Carvalho and Freitas simultaneously reported that partially hydrolyzed guar gum was able to ameliorate iron deficiency anemia by improving iron absorption and iron metabolism status [3, 27]. Increased Fe²⁺ and SF levels in renal anemia patients after oral administration of DF were also found in our study, but the levels of sTfR were not notably affected. DF also had no obvious effect on hepcidin (an iron metabolism-related regulatory peptide). This is consistent with the findings of a recent systematic review that stated that DF has heterogeneous effects in ameliorating anemia by improving iron metabolism [5]. However, as stated in this systematic review, given the complexity of body iron metabolism and the variability in the design of individual studies, we cannot ignore the role of DF in improving iron metabolism and, in particular, its function of modulating gut bacterial biomarkers [5]. Our experimental data implicate the relevance of DF, the gut microbiota, SCFA, and iron metabolism, and our future research will investigate these associations.

As reported by Torres et al., BA stimulated Chinese hamster ovary cells to synthesize EPO in vitro, and BA was an important metabolite of DF and one of the most changed metabolites in our study [36]. Our study did not find a significant decrease in EPO consumption, although such a trend was indecated. As mentioned earlier, this trend could also be the result of improved iron metabolism or the effect of DF and its metabolites on erythropoietin receptor (EPOR) [37, 38]. However, whether DF can affect the synthesis of EPO in CKD patients has not been reported. Our research shows the potential function of DF in improving renal anemia by regulating EPO and EPO-related pathways, which is worthy of further study.

SCFAs have the potential to regulate bone marrow hematopoiesis. G protein coupled receptors (GPCRs) are the targets of SCFA. Trompette et al. found that propionate has the ability to regulate the differentiation of bone marrow hematopoietic cells by activating GPCR41, which mainly manifested as an increase in bone marrow-derived dendritic cells [22]. Accordingly, Docampo et al. found that butyrate can maintain the immune function of bone marrow-derived T cells by activating GPCR109A [39]. However, whether SCFAs can induce the differentiation of bone marrow erythroid hematopoietic cells by GPCRs needs further study.

Although our study raises the possibility that DF, the gut microbiota, and SCFA may improve renal anemia through the iron metabolism pathway or EPO-related pathway, there are still some limitations in this study. First, although the role of DF in health has been continually demonstrated, whether differences exist for different DFs has not confirmed by our study. We chose one mixture of DF, but whether other kinds of DF have similar or even better effects requires further research. Second, our study did not evaluate the long-term effects of DF on the gut microbiota. For example, whether the gut microbiota would be restored to its deleterious status after patients stopped using DF supplements was not investigated. How to use DF supplements to maintain the stability of the gut microbiota to achieve long-term therapeutic effects may be an interesting direction for future research. Third, our study did not clarify the exact mechanism by which DF improves renal anemia, but it provides a possible direction for our next basic research.

DF ameliorates renal anemia by modulating the prebiotic activity of the gut microbiota and SCFAs and this effect warrants in-depth exploration in larger cohorts.