nach oben

World Journal of Urology

Erschienen in:

01.06.2013 | Original Article

The protective effects of grape seed extract on MDA, AOPP, apoptosis and eNOS expression in testicular torsion: an experimental study

verfasst von: Firuze Bayatli, Derya Akkuş, Eser Kilic, Recep Saraymen, Mehmet Fatih Sönmez

Erschienen in: World Journal of Urology | Ausgabe 3/2013

Einloggen, um Zugang zu erhalten

Abstract

Objectives

Grape seed proanthocyanidin extract (GSPE) is a potent antioxidant and a free radical scavenger. This study was designed to determine whether GSPE could protect against dysfunction and oxidative stress induced by torsion–detorsion injury in rat testis.

Methods

A total of 45 male Wistar albino rats were divided into five groups: control group, sham group, torsion–detorsion (T/D) group, T/D + GSPE group, GSPE group. GSPE was administrated 100 mg/kg/day with oral gavage over seven days before torsion. Testicular torsion was performed for 2 h, and afterward, detorsion was performed for 2 h. The rats were decapitated under ketamine anesthesia, and their testes tissues were removed. Tissue malondialdehyde, advanced oxidation protein products levels, eNOS expression, apoptosis and histopathological damage scores were then compared.

Results

Testicular torsion–detorsion caused significant increases in malondialdehyde level, apoptosis and eNOS expression level and caused a significant decrease in advanced oxidation protein product levels and testicular spermatogenesis in ipsilateral testes. GSPE prevented the rise in malondialdehyde, apoptosis and eNOS expression and improved testicular morphology and Johnsen’s score.

Conclusions

As a result, testicular torsion gives rise to serious damage in testes and GSPE is a potent antioxidant agent in preventing testicular injury.

Dokmeci D, Kanter M, Inan M, Aydogdu N, Basaran UN, Yalcin O, Turan FN (2007) Protective effects of ibuprofen on testicular torsion/detorsion-induced ischemia/reperfusion injury in rats. Arch Toxicol 81(9):655–663PubMedCrossRef

Tamamura M, Saito M, Kinoshita Y, Shimizu S, Satoh I, Shomori K, Dimitriadis F, Satoh K (2010) Protective effect of edaravone, a free-radical scavenger, on ischaemia-reperfusion injury in the rat testis. BJU Int 105(6):870–876PubMedCrossRef

Turkmen S, Mentese A, Karaguzel E, Karaca Y, Kucuk A, Uzun A, Yulug E, Turedi S (2012) A comparison of the effects of N-acetylcysteine and ethyl pyruvate on experimental testicular ischemia-reperfusion injury. Fertil Steril 98(3):626–631PubMedCrossRef

Ariga T (2004) The antioxidative function, preventive action on disease and utilization of proanthocyanidins. BioFactors 21(1–4):197–201PubMedCrossRef

Guler A, Sahin MA, Yucel O, Yokusoglu M, Gamsizkan M, Ozal E, Demirkilic U, Arslan M (2011) Proanthocyanidin prevents myocardial ischemic injury in adult rats. Med Sci Monit 17(11):BR326–BR331PubMedCrossRef

Ulusoy S, Ozkan G, Yucesan FB, Ersoz S, Orem A, Alkanat M, Yulug E, Kaynar K, Al S (2012) Anti-apoptotic and anti-oxidant effects of grape seed proanthocyanidin extract in preventing cyclosporine A-induced nephropathy. Nephrology (Carlton) 17(4):372–379CrossRef

Su L, Deng Y, Zhang Y, Li C, Zhang R, Sun Y, Zhang K, Li J, Yao S (2011) Protective effects of grape seed procyanidin extract against nickel sulfate-induced apoptosis and oxidative stress in rat testes. Toxicol Mech Methods 21(6):487–494PubMedCrossRef

Kanter M (2010) Protective effects of melatonin on testicular torsion/detorsion-induced ischemia-reperfusion injury in rats. Exp Mol Pathol 89(3):314–320PubMedCrossRef

Ohkawa H, Ohishi N, Yagi K (1979) Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95(2):351–358PubMedCrossRef

10.

Witko-Sarsat V, Friedlander M, Capeillere-Blandin C, Nguyen-Khoa T, Nguyen AT, Zingraff J, Jungers P, Descamps-Latscha B (1996) Advanced oxidation protein products as a novel marker of oxidative stress in uremia. Kidney Int 49(5):1304–1313PubMedCrossRef

11.

Johnsen SG (1970) Testicular biopsy score count—a method for registration of spermatogenesis in human testes: normal values and results in 335 hypogonadal males. Hormones 1(1):2–25PubMedCrossRef

12.

Turner TT, Brown KJ (1993) Spermatic cord torsion: loss of spermatogenesis despite return of blood flow. Biol Reprod 49(2):401–407PubMedCrossRef

13.

Uguralp S, Bay Karabulut A, Mizrak B, Kaymaz F, Kiziltay A, Hasirci N (2004) The effect of sustained and local administration of epidermal growth factor on improving bilateral testicular tissue after torsion. Urol Res 32(5):323–331PubMedCrossRef

14.

Dokmeci D, Inan M, Basaran UN, Yalcin O, Aydogdu N, Turan FN, Uz YH (2007) Protective effect of l-carnitine on testicular ischaemia-reperfusion injury in rats. Cell Biochem Funct 25(6):611–618PubMedCrossRef

15.

Aitken RJ, Roman SD (2008) Antioxidant systems and oxidative stress in the testes. Oxid Med Cell Longev 1(1):15–24PubMedCrossRef

16.

Turner TT, Tung KS, Tomomasa H, Wilson LW (1997) Acute testicular ischemia results in germ cell-specific apoptosis in the rat. Biol Reprod 57(6):1267–1274PubMedCrossRef

17.

Aksoy H, Yapanoglu T, Aksoy Y, Ozbey I, Turhan H, Gursan N (2007) Dehydroepiandrosterone treatment attenuates reperfusion injury after testicular torsion and detorsion in rats. J Pediatr Surg 42(10):1740–1744PubMedCrossRef

18.

Yildiz H, Durmus AS, Simsek H, Yaman M (2012) Dose-dependent protective effect of sildenafil citrate on testicular injury after torsion/detorsion in rats. Andrologia 44(Suppl 1):300–306PubMedCrossRef

19.

Taskara E, Gor A, Kutlu O, Karaguzel E, Cobanoglu U, Topbas M, Senel AC (2011) Does propofol prevent testicular ischemia-reperfusion injury due to torsion in the long term? Pediatr Surg Int 27(9):1003–1007PubMedCrossRef

20.

Sehirli O, Ozel Y, Dulundu E, Topaloglu U, Ercan F, Sener G (2008) Grape seed extract treatment reduces hepatic ischemia-reperfusion injury in rats. Phytother Res 22(1):43–48PubMedCrossRef

21.

Guven C, Borcek AO, Cemil B, Kurt G, Yildirim Z, Ucankus NL, Kilic N, Ceviker N (2010) Neuroprotective effects of infliximab in experimental spinal cord ischemic injury. J Clin Neurosci 17(12):1563–1567PubMedCrossRef

22.

Skvarilova M, Bulava A, Stejskal D, Adamovska S, Bartek J (2005) Increased level of advanced oxidation products (AOPP) as a marker of oxidative stress in patients with acute coronary syndrome. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub 149(1):83–87PubMedCrossRef

23.

Billig H, Furuta I, Rivier C, Tapanainen J, Parvinen M, Hsueh AJ (1995) Apoptosis in testis germ cells: developmental changes in gonadotropin dependence and localization to selective tubule stages. Endocrinology 136(1):5–12PubMedCrossRef

24.

Shiraishi K, Naito K, Yoshida K (2001) Nitric oxide promotes germ cell necrosis in the delayed phase after experimental testicular torsion of rat. Biol Reprod 65(2):514–521PubMedCrossRef

25.

Aktoz T, Kanter M, Aktas C (2010) Protective effects of quercetin on testicular torsion/detorsion-induced ischaemia-reperfusion injury in rats. Andrologia 42(6):376–383PubMedCrossRef

26.

Moon C, Ahn M, Kim S, Yasuzumi F, Shin T (2005) Increased expression of both constitutive and inducible forms of nitric oxide synthase in the delayed phase of acute experimental testicular torsion. J Vet Med Sci 67(4):453–456PubMedCrossRef

27.

Kono T, Saito M, Kinoshita Y, Satoh I, Shinbori C, Satoh K (2006) Real-time monitoring of nitric oxide and blood flow during ischemia-reperfusion in the rat testis. Mol Cell Biochem 286(1–2):139–145PubMedCrossRef

28.

Shiraishi K, Yoshida K, Naito K (2003) Activation of endothelial nitric oxide synthase in contralateral testis during unilateral testicular torsion in rats. Arch Androl 49(3):179–190PubMedCrossRef

29.

Akgul T, Karaguzel E, Surer H, Yagmurdur H, Ayyildiz A, Ustun H, Germiyanoglu C (2009) Ginkgo biloba (EGB 761) affects apoptosis and nitric-oxide synthases in testicular torsion: an experimental study. Int Urol Nephrol 41(3):531–536PubMedCrossRef

30.

Kanter M (2011) Protective effects of Ginkgo biloba (EGb 761) on testicular torsion/detorsion-induced ischemia-reperfusion injury in rats. Exp Mol Pathol 91(3):708–713PubMedCrossRef

Titel: The protective effects of grape seed extract on MDA, AOPP, apoptosis and eNOS expression in testicular torsion: an experimental study
verfasst von: Firuze Bayatli
Derya Akkuş
Eser Kilic
Recep Saraymen
Mehmet Fatih Sönmez
Publikationsdatum: 01.06.2013
Verlag: Springer-Verlag
Erschienen in: World Journal of Urology / Ausgabe 3/2013
Print ISSN: 0724-4983
Elektronische ISSN: 1433-8726
DOI: https://doi.org/10.1007/s00345-013-1049-8

Neu im Fachgebiet Urologie

Ambulantisierung: Erste Erfahrungen mit dem Hybrid-DRG

02.05.2024 DCK 2024 Kongressbericht

Die Hybrid-DRG-Verordnung soll dazu führen, dass mehr chirurgische Eingriffe ambulant durchgeführt werden, wie es in anderen Ländern schon länger üblich ist. Die gleiche Vergütung im ambulanten und stationären Sektor hatten Niedergelassene schon lange gefordert. Aber die Umsetzung bereitet ihnen doch Kopfzerbrechen.

Sind Frauen die fähigeren Ärzte?

30.04.2024 Gendermedizin Nachrichten

Patienten, die von Ärztinnen behandelt werden, dürfen offenbar auf bessere Therapieergebnisse hoffen als Patienten von Ärzten. Besonders gilt das offenbar für weibliche Kranke, wie eine Studie zeigt.

Harninkontinenz: Netz-Op. erfordert über lange Zeit intensive Nachsorge

30.04.2024 Harninkontinenz Nachrichten

Frauen mit Belastungsinkontinenz oder Organprolaps sind nach einer Netz-Operation keineswegs beschwerdefrei. Vielmehr scheint die Krankheitslast weiterhin hoch zu sein, sogar höher als von harninkontinenten Frauen, die sich nicht haben operieren lassen.

Adjuvante Immuntherapie verlängert Leben bei RCC

25.04.2024 Nierenkarzinom Nachrichten

Nun gibt es auch Resultate zum Gesamtüberleben: Eine adjuvante Pembrolizumab-Therapie konnte in einer Phase-3-Studie das Leben von Menschen mit Nierenzellkarzinom deutlich verlängern. Die Sterberate war im Vergleich zu Placebo um 38% geringer.

Update Urologie

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert.

Newsletter bestellen

Springer Medizin

Abstract

Objectives

Methods

Results

Conclusions

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 3/2013

Prolapse surgery in octogenarians: are we pushing the limits too far?

Urological surgery in patients with hemorrhagic bleeding disorders Hemophilia A, Hemophilia B, von Willebrand disease: a retrospective study with matched pairs analysis

Effect of minimizing tension during robotic-assisted laparoscopic radical prostatectomy on urinary function recovery

Outcomes and complications of pelvic lymph node dissection during robotic-assisted radical prostatectomy