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International Orthopaedics
Erschienen in: International Orthopaedics 5/2017

16.02.2017 | Original Paper

Transcutaneous carbon dioxide application accelerates muscle injury repair in rat models

verfasst von: Shiho Akahane, Yoshitada Sakai, Takeshi Ueha, Hanako Nishimoto, Miho Inoue, Takahiro Niikura, Ryosuke Kuroda

Erschienen in: International Orthopaedics | Ausgabe 5/2017

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Abstract

Purpose

Skeletal muscle injuries are commonly observed in sports and traumatology medicine. Previously, we demonstrated that transcutaneous application of carbon dioxide (CO2) to lower limbs increased the number of muscle mitochondria and promoted muscle endurance. Therefore, we aimed to investigate whether transcutaneous CO2 application could enhance recovery from muscle injury.

Methods

Tibialis anterior muscle damage was induced in 27 Sprague Dawley rats via intramuscular injection of bupivacaine. After muscle injury, rats were randomly assigned to transcutaneous CO2-treated or -untreated groups. From each group, three rats were sacrificed at weeks one, two, four and six. At each time point, histology and immunofluorescence analyses were performed, and changes in muscle weight, muscle weight/body weight ratio, muscle fibre circumference, gene expression levels and capillary density were measured.

Results

Injured muscle fibres were completely repaired at week six in the CO2-treated group but only partially repaired in the untreated group. The repair of basement and plasma membranes did not differ significantly between groups. However, expression levels of genes and proteins related to muscle protein synthesis were significantly higher in the CO2−treated group and significantly more capillaries four weeks after injury.

Conclusion

Transcutaneous CO2 application can accelerate recovery after muscle injury in rats.
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Metadaten
Titel
Transcutaneous carbon dioxide application accelerates muscle injury repair in rat models
verfasst von
Shiho Akahane
Yoshitada Sakai
Takeshi Ueha
Hanako Nishimoto
Miho Inoue
Takahiro Niikura
Ryosuke Kuroda
Publikationsdatum
16.02.2017
Verlag
Springer Berlin Heidelberg
Erschienen in
International Orthopaedics / Ausgabe 5/2017
Print ISSN: 0341-2695
Elektronische ISSN: 1432-5195
DOI
https://doi.org/10.1007/s00264-017-3417-2

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