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Erschienen in:
01.02.2009 | Original Article
Upregulation of mRNA expression of MCP-1 by TGF-β1 in fibroblast cells from Peyronie’s disease
Erschienen in: World Journal of Urology | Ausgabe 1/2009
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Introduction
Peyronie’s disease (PD) is a localized connective tissue disorder of the penile tunica albuginea (TA) with a still obscure etiopathology. Recent studies from our laboratory have demonstrated differences in Smad3 and Smad4 gene expression of PD-fibroblasts and non-PD-fibroblasts after stimulation with recombinant TGF-β1 for 1 h. In the present study, we investigated gene expression of Smad2–Smad4 and Smad7 up to 6 h after stimulation with TGF-β1. As a positive control, MCP-1 gene expression was monitored.
Materials and methods
Cells with fibroblast characteristics were isolated from seven PD plaques and three TA controls. The cells were incubated with recombinant TGF-β1 for 2–6 h and expression of Smad2–Smad4, Smad7, and monocyte chemotactic protein-1 (MCP-1) was determined by quantitative real-time PCR.
Results
TGF-β1 treatment resulted in a statistically significant up-regulation of Smad7 and MCP-1 gene expression. Smad7 expression was increased after 2 h (P < 0.001) and was still high after 4 h (P < 0.05). No significant differences between fibroblasts from PD-patients compared to non-PD-patients were observed. MCP-1 peaked after 4 h (P < 0.001) and remained high up to 6 h (P < 0.01). PD-fibroblasts revealed a significantly increased MCP-1 gene expression compared to non-PD-fibroblasts (P = 0.013) after 2 h and remained significantly different also after 6 h (P = 0.038). Gene expression of Smad2–Smad4 did not change during stimulation with TGF-β1.
Conclusion
In conclusion, analysis of MCP-1 expression might be a useful marker for Peyronie’s disease.