Our study demonstrates that, although a majority of PC lung metastases were PSMA-positive, a considerable share of metastases was PSMA-negative and could therefore not be detected directly by
68Ga-PSMA-PET. Up to now, the largest study investigating the
68Ga-PSMA-PET imaging of PC pulmonary metastases and primary lung cancer was conducted by Pyka et al., with 45 patients and 89 lesions [
20]. Pyka et al. demonstrated that, due to the high tracer uptake in lung cancer, differentiation between primary lung cancer and lung metastases by SUV analysis was not possible [
20]. Within their cohort, mean SUV
max of lung metastases was 4.4±3.9, which is consistent with the SUV
max calculated in our cohort. Although Pyka et al. did not explicitly differentiate between PSMA-positive and PSMA-negative metastases, they observed a great heterogeneity of tracer uptake in pulmonary metastases; many metastases showed only a faint tracer uptake [
20]. This finding is also consistent with our study, in which 27.5% of metastases were PSMA-negative. A possible explanation for the difference of
68Ga-PSMA-HBED-CC uptake in lung metastases is the diversity of phenotypes in metastases; especially neuroendocrine trans-differentiation has been identified as a main factor for the loss of PSMA-expression in visceral metastases [
26]. This can be explained by the fact that a large part of neuroendocrine prostate cancer cells does not express generic PC biomarkers such as P501S, PSMA, and PSA [
27]. Neuroendocrine trans-differentiation was proven histologically in the single patient of our study who had only PSMA-negative pulmonary metastases. A case report by Shetty et al. of a non-PSMA-avid PC lung metastasis suggests that an uncommon variant of the primary PC, in this case ductal adenocarcinoma, can be another cause for missing PSMA-expression [
28]. However, the detection rate of the more common lymph node and bone metastases in PSMA-PET appear to be much higher. Schwenck et al. reported detection rates of 94% for lymph node and 98% for bone metastases [
29]. Regarding the overall incidence of PSMA-negativity in prostate cancer, Mannweiler et al. found 5% of primary prostate cancer and 15% of prostate cancer metastases to be PSMA-negative in immunohistochemistry [
30].
Compared to most other tissues, background tracer uptake of the lungs in
68Ga-PSMA-PET is low [
31,
32]. Therefore, PSMA-positive lung metastases are clearly visible and detectable as a result of a high lesion-to-background contrast. The low PSMA-expression in the normal lung parenchyma has been confirmed immunohisto-chemically, since bronchioles and terminal bronchioles of normal lung did not stain [
33].
It is thought that tracer uptake in pulmonary opacities might not be associated with an increased avidity of the lesion, but due to increased capillary penetration caused by inflammation, which results in a higher tracer activity in the interstitial space [
22]. However, another explanation could be the PSMA-expression in the neovasculature of physiologic regenerative and reparative conditions as shown by Gordon et al. [
34]. This question could be a subject of investigation for future studies.