The online version of this article (doi:10.1186/1756-9966-33-55) contains supplementary material, which is available to authorized users.
The authors declare that they have no competing interests.
PC conceived and designed the experiments. BZ, HXC, DW, YK and GYL performed the experiments. BZ, HXC and JX analyzed the data. BZ wrote the paper. PC supervised the whole experimental work and revised the manuscript. All authors read and approved the manuscript.
To understand the involvement of structural maintenance of chromosome 4 (SMC4) in the development and progression of hepatocellular carcinoma (HCC).
Real-time quantitative PCR and Western Blotting were applied to measure the expression of SMC4 in HCC samples and cell lines. The tumor-promoting effect of SMC4 was determined by WST-1, soft agar colony formation, cell motility and invasion assays. The SMC4 target signal pathway was identified by luciferase reporter and real-time quantitative PCR assays.
The upregulation of SMC4 was frequently detected in HCC samples and cell lines. Functional assays demonstrated that SMC4 could effectively promote tumor cell growth rate, colony formation in soft agar, wound-healing and invasion. Further studies showed that increased miR-219 levels caused a significant decrease in the SMC4 expression, and SMC4 inhibitor downregulated JAK2/Stat3 expression at both the mRNA and protein levels.
Our findings provide new insight into SMC4 function and the mechanisms of growth and invasion of HCC.
Additional file 1: Recombinant plasmid construct report.(DOC 35 KB)13046_2014_783_MOESM1_ESM.doc
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- A novel miR-219-SMC4-JAK2/Stat3 regulatory pathway in human hepatocellular carcinoma
- BioMed Central
Journal of Experimental & Clinical Cancer Research
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