Skip to main content
main-content

01.12.2014 | Research | Ausgabe 1/2014 Open Access

Journal of Experimental & Clinical Cancer Research 1/2014

A novel miR-219-SMC4-JAK2/Stat3 regulatory pathway in human hepatocellular carcinoma

Zeitschrift:
Journal of Experimental & Clinical Cancer Research > Ausgabe 1/2014
Autoren:
Bo Zhou, Hongxu Chen, Dong Wei, Yi Kuang, Xiaobiao Zhao, Guangyao Li, Jun Xie, Ping Chen
Wichtige Hinweise

Electronic supplementary material

The online version of this article (doi:10.​1186/​1756-9966-33-55) contains supplementary material, which is available to authorized users.

Competing interests

The authors declare that they have no competing interests.

Authors’ contributions

PC conceived and designed the experiments. BZ, HXC, DW, YK and GYL performed the experiments. BZ, HXC and JX analyzed the data. BZ wrote the paper. PC supervised the whole experimental work and revised the manuscript. All authors read and approved the manuscript.

Abstract

Background

To understand the involvement of structural maintenance of chromosome 4 (SMC4) in the development and progression of hepatocellular carcinoma (HCC).

Methods

Real-time quantitative PCR and Western Blotting were applied to measure the expression of SMC4 in HCC samples and cell lines. The tumor-promoting effect of SMC4 was determined by WST-1, soft agar colony formation, cell motility and invasion assays. The SMC4 target signal pathway was identified by luciferase reporter and real-time quantitative PCR assays.

Results

The upregulation of SMC4 was frequently detected in HCC samples and cell lines. Functional assays demonstrated that SMC4 could effectively promote tumor cell growth rate, colony formation in soft agar, wound-healing and invasion. Further studies showed that increased miR-219 levels caused a significant decrease in the SMC4 expression, and SMC4 inhibitor downregulated JAK2/Stat3 expression at both the mRNA and protein levels.

Conclusions

Our findings provide new insight into SMC4 function and the mechanisms of growth and invasion of HCC.
Zusatzmaterial
Additional file 1: Recombinant plasmid construct report.(DOC 35 KB)
13046_2014_783_MOESM1_ESM.doc
Authors’ original file for figure 1
13046_2014_783_MOESM2_ESM.tif
Authors’ original file for figure 2
13046_2014_783_MOESM3_ESM.tif
Authors’ original file for figure 3
13046_2014_783_MOESM4_ESM.tiff
Authors’ original file for figure 4
13046_2014_783_MOESM5_ESM.tiff
Authors’ original file for figure 5
13046_2014_783_MOESM6_ESM.tiff
Authors’ original file for figure 6
13046_2014_783_MOESM7_ESM.tiff
Authors’ original file for figure 7
13046_2014_783_MOESM8_ESM.tiff
Literatur
Über diesen Artikel

Weitere Artikel der Ausgabe 1/2014

Journal of Experimental & Clinical Cancer Research 1/2014 Zur Ausgabe

Neu im Fachgebiet Onkologie

Mail Icon II Newsletter

Bestellen Sie unseren kostenlosen Newsletter Update Onkologie und bleiben Sie gut informiert – ganz bequem per eMail.

Bildnachweise