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01.11.2015 | Original Article

A porcine circovirus-2 mutant isolated in Brazil contains low-frequency substitutions in regions of immunoprotective epitopes in the capsid protein

verfasst von: Rafael Locatelli Salgado, Pedro Marcus Pereira Vidigal, Natalia F. Gonzaga, Luiz F. L. de Souza, Marcelo D. Polêto, Thiago Souza Onofre, Monique R. Eller, Carlos Eduardo Real Pereira, Juliana L. R. Fietto, Gustavo C. Bressan, Roberto M. C. Guedes, Márcia R. Almeida, Abelardo Silva Júnior

Erschienen in: Archives of Virology | Ausgabe 11/2015

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Abstract

Porcine circovirus-2 (PCV2) is the etiologic agent of several diseases in pigs, including multi-systemic wasting syndrome (PMWS). In this work, a new mutant PCV2b was isolated from PMWS-affected pigs on a Brazilian farm. Its genome showed high sequence similarity (>99 % identity) to those from a group of emerging mutants isolated from cases of PMWS outbreaks in vaccinated pigs in China, the USA and South Korea. Here, we show that these isolates share a combination of low-frequency substitutions (single amino acid polymorphisms with a frequency of ≤25 %) in the viral capsid protein, mainly in regions of immunoprotective epitopes, and an additional lysine residue at position 234. These isolates were phylogenetically grouped in the PCV2b clade, reinforcing the idea of the emergence of a new group of mutants PCV2b associated with outbreaks worldwide. The identification of these polymorphisms in the viral capsid highlights the importance of considering these isolates for the development of more-effective vaccines.

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Titel: A porcine circovirus-2 mutant isolated in Brazil contains low-frequency substitutions in regions of immunoprotective epitopes in the capsid protein
verfasst von: Rafael Locatelli Salgado
Pedro Marcus Pereira Vidigal
Natalia F. Gonzaga
Luiz F. L. de Souza
Marcelo D. Polêto
Thiago Souza Onofre
Monique R. Eller
Carlos Eduardo Real Pereira
Juliana L. R. Fietto
Gustavo C. Bressan
Roberto M. C. Guedes
Márcia R. Almeida
Abelardo Silva Júnior
Publikationsdatum: 01.11.2015
Verlag: Springer Vienna
Erschienen in: Archives of Virology / Ausgabe 11/2015
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-015-2567-z

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